Preparation method of hog cholera virus antigen for improving virus content or reaction rate of animal body

A swine fever virus and animal body technology, applied in the directions of virus antigen components, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the uneven response rate of rabbit body, low animal utilization rate, and animal requirements. Strict and other problems, to achieve the effect of improving the reaction rate of rabbit body, improving product titer, and reducing product side reactions

Pending Publication Date: 2021-05-28
兆丰华生物科技(南京)有限公司 +3
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Problems solved by technology

[0003] The above method has the following deficiencies: 1. The response rate of the rabbit body is uneven. The high response rate can reach about 85%, and the worst response rate is only below 45%; ...

Method used

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  • Preparation method of hog cholera virus antigen for improving virus content or reaction rate of animal body
  • Preparation method of hog cholera virus antigen for improving virus content or reaction rate of animal body
  • Preparation method of hog cholera virus antigen for improving virus content or reaction rate of animal body

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preparation example Construction

[0035] A method for preparing a swine fever virus antigen that improves animal virus content or reaction rate, comprising the following steps:

[0036] 1) Take 40g of classical swine fever virus (rabbitization attenuated strain) to produce seed virus, first add 40ml of normal saline, grind it into a fine granular suspension with a mortar, then dilute it with normal saline to 5-50 times suspension, 80-50 times Filter through a 90-mesh filter, and collect the filtrate (i.e. the suspension of the attenuated strain of classical swine fever lathe virus), for subsequent use;

[0037] 2) Inoculate the filtrate (i.e. the virus suspension of the attenuated strain of CSF) into rabbits. The inoculation methods include intravenous injection, intramuscular injection, subcutaneous injection, intraperitoneal injection, intradermal injection, intrapulmonary injection, eye drops, oral administration, nasal drops, At least two inoculation methods in the spray method;

[0038] Two to three days...

Embodiment 1

[0058] Preparation method of virus antigen of classical swine fever lanotype attenuated strain

[0059] 1) Take 40g of classical swine fever virus (rabbitization attenuated strain) to produce seed virus, first add 40ml of normal saline, grind it into a fine particle suspension with a mortar, then dilute it with normal saline to 10 times suspension, filter with 80 mesh Filter, collect filtrate (being the attenuated virus suspension of classical swine fever lathe virus), for subsequent use;

[0060] 2) Inoculate the filtrate (i.e. the virus suspension of the attenuated strain of CSF) into the ear vein of 1 ml / rabbit, subcutaneously inject 1 ml / rabbit, and intramuscularly inject 1 ml / rabbit. Three days before inoculation, the temperature of the rabbits was measured once a day in the morning and afternoon, and the body temperature was between 38.0°C and 40.0°C, and the temperature fluctuation in the morning and afternoon was less than 1.0°C. The requirements for big rabbits ar...

Embodiment 2

[0067] Preparation method of virus antigen of classical swine fever lanotype attenuated strain

[0068] 1) Take 40g of classical swine fever virus (rabbitization attenuated strain) to produce seed virus, first add 40ml of normal saline, grind it into a fine granular suspension with a mortar, then dilute it with normal saline to 9 times of suspension, filter with 90 mesh Filter, collect filtrate (being the attenuated virus suspension of classical swine fever lathe virus), for subsequent use

[0069] 2) Inoculate the filtrate (i.e. virus suspension of the attenuated strain of CSF) into the ear vein with 1ml / rat, subcutaneously inject 1ml / rat, intramuscularly inject 1ml / rat, and drip nasally with 1ml / rat. Three days before inoculation, the temperature of the rabbits was measured once a day in the morning and afternoon, and the body temperature was between 38.0°C and 40.0°C, and the temperature fluctuation in the morning and afternoon was less than 1.0°C. Table 2 for the quality re...

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Abstract

The invention discloses a preparation method of a hog cholera virus antigen for improving the virus content or reaction rate of an animal body. The method comprises the steps: 1) grinding, diluting and filtering a hog cholera virus production seed virus for later use; (2) inoculating the filtrate (namely the hog cholera lapinized virus strain virus suspension) into rabbit bodies in at least two inoculation modes; (3) after inoculating for 24 hours, measuring the temperature once every 6 hours, and recording the temperature of each rabbit body; (4) collecting splenic tissues of shaped hot rabbit bodies after 24-48 hours; 5) immunizing the rest rabbit bodies which is not shaped and hot within 48 hours with the filtrate (namely the hog cholera lapinized virus strain virus suspension) again, wherein the inoculation mode is the same as that of the step 2); 6) measuring the temperature once every 6 hours after the second inoculation, and recording the temperature of each rabbit body; and 7), collecting splenic tissues of rabbit bodies which is shaped and hot 24-48 hours after secondary inoculation is conducted, and thus obtaining the hog cholera virus antigen. According to the method, the method of being more than 1 immunization and more than one immunization way is adopted, the viruses are propagated, virus-containing tissues are collected, the animal body utilization rate is increased to 95% or above, and the virus content is increased by 1.5-6 times or above compared with the prior art.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a swine fever virus antigen for improving animal body virus content or reaction rate. Background technique [0002] The production process of live swine fever vaccine (rabbit source) is to inoculate healthy rabbits in the ear vein of the attenuated strain of hog fever lathe virus, harvest the spleen and mesenteric lymph nodes of the infected rabbits, add appropriate stabilizers, and freeze and dry them in a vacuum. The rabbit immunization route is a single ear vein inoculation, the reaction rate of the rabbit body is between 45% and 85%, and the tissue toxicity is about 20000RID / g~80000RID / g. [0003] The above method has the following deficiencies: 1. The response rate of the rabbit body is uneven. The high response rate can reach about 85%, and the worst response rate is only below 45%; 2. There is still a large room for improvement in the tissue ...

Claims

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Application Information

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IPC IPC(8): A61K39/187A61P31/14
CPCA61K39/12A61K2039/54A61K2039/552A61P31/14C12N2770/24334
Inventor 赵华娥林晓荣
Owner 兆丰华生物科技(南京)有限公司
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