Preparation method of hog cholera virus antigen for improving virus content or reaction rate of animal body

Abstract

The invention discloses a preparation method of a hog cholera virus antigen for improving the virus content or reaction rate of an animal body. The method comprises the steps: 1) grinding, diluting and filtering a hog cholera virus production seed virus for later use; (2) inoculating the filtrate (namely the hog cholera lapinized virus strain virus suspension) into rabbit bodies in at least two inoculation modes; (3) after inoculating for 24 hours, measuring the temperature once every 6 hours, and recording the temperature of each rabbit body; (4) collecting splenic tissues of shaped hot rabbit bodies after 24-48 hours; 5) immunizing the rest rabbit bodies which is not shaped and hot within 48 hours with the filtrate (namely the hog cholera lapinized virus strain virus suspension) again, wherein the inoculation mode is the same as that of the step 2); 6) measuring the temperature once every 6 hours after the second inoculation, and recording the temperature of each rabbit body; and 7), collecting splenic tissues of rabbit bodies which is shaped and hot 24-48 hours after secondary inoculation is conducted, and thus obtaining the hog cholera virus antigen. According to the method, the method of being more than 1 immunization and more than one immunization way is adopted, the viruses are propagated, virus-containing tissues are collected, the animal body utilization rate is increased to 95% or above, and the virus content is increased by 1.5-6 times or above compared with the prior art.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a swine fever virus antigen for improving animal body virus content or reaction rate. Background technique [0002] The production process of live swine fever vaccine (rabbit source) is to inoculate healthy rabbits in the ear vein of the attenuated strain of hog fever lathe virus, harvest the spleen and mesenteric lymph nodes of the infected rabbits, add appropriate stabilizers, and freeze and dry them in a vacuum. The rabbit immunization route is a single ear vein inoculation, the reaction rate of the rabbit body is between 45% and 85%, and the tissue toxicity is about 20000RID / g~80000RID / g. [0003] The above method has the following deficiencies: 1. The response rate of the rabbit body is uneven. The high response rate can reach about 85%, and the worst response rate is only below 45%; 2. There is still a large room for improvement in the tissue ...

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Problems solved by technology

[0003] The above method has the following deficiencies: 1. The response rate of the rabbit body is uneven. The high response rate can reach about 85%, and the worst response rate is only below 45%; 2. There is still a large room for improvement in the tissue toxin content; 3. The utilization rate of animals is low; 4. Strict requirements for animals, such as animal age, weight, antibody level, etc.

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