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A kind of vaccine composition and its preparation method and application

A vaccine composition, the technology of Haemophilus suis, applied in the field of vaccine composition and its preparation and application, can solve the problems of maternal antibody interference, not reducing the immune effect, etc., and achieve the effect of controllable quality and good immune effect

Active Publication Date: 2018-03-02
PULIKE BIOLOGICAL ENG INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The inventors have found that if nasal drop immunization is carried out with the vaccine composition of swine fever, porcine pseudorabies and Haemophilus parasuis, the problem of maternal antibody interference can be solved, and a good effect on swine fever, porcine pseudorabies and Haemophilus parasuis can be produced. immune protection; when applied, live vaccines for swine fever, porcine pseudorabies and inactivated Haemophilus parasuis vaccines can be used in the form of diluted live vaccines with inactivated vaccines, and can also be mixed in a certain proportion and then added with a lyoprotectant for freeze-drying. , easy to transport and does not reduce the immune effect; in addition, it was also found that the inactivated Haemophilus parasuis vaccine has an immune-enhancing effect on swine fever and porcine pseudorabies vaccine, and the latter antigen can be halved to achieve the same effect as a single vaccine

Method used

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  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Preparation of Anti-Swine Fever Virus, Porcine Pseudorabies Virus and Haemophilus parasuis Vaccine Composition

[0047] 1. Preparation and testing of CSFV antigen solution

[0048] (1) Treat ST cells (purchased from ATCC) (American typeculture collection, American Type Culture Collection) that have grown into a good monolayer with high sensitivity to classical swine fever virus with 0.125wt% trypsin and 0.03wt% EDTA (B Diaminetetraacetic acid) digestion solution was digested and dispersed, the cells were counted, and after inoculation at a suitable density, MEM containing 3wt%-5wt% FBS (fetal bovine serum) (purchased from PAA Company, batch number A15110-1462) was added ( Purchased from GIBCO Company, batch number 856833), at the same time add seed virus according to M.O.I. (multiplicity of infection) = 0.1-0.2 inoculation dose, and place in an incubator at 34-37°C for cultivation.

[0049] (2) After three days of cultivation, the first poisoning was carri...

Embodiment 2

[0069] Example 2 Correlative inspection of classical swine fever virus, porcine pseudorabies virus and Haemophilus parasuis vaccine

[0070] 1. Physical property test, sterility test

[0071] Vaccines 1 to 8 passed the physical property test and sterility test, and the detailed results are shown in Table 3:

[0072] Table 3 Vaccine physical properties test and sterility test

[0073]

[0074]

[0075] Note: T.G means thioglycolate medium, G.A means casease agar medium, G.P glucose-peptone medium; "-" means sterile growth.

[0076] 2 safety test

[0077] With 45 piglets negative for classical swine fever virus, porcine pseudorabies virus and Haemophilus parasuis antigen and antibody at the age of 2 to 3 weeks, 5 pigs in groups 1 to 3 were inoculated with 2 parts of inactivated vaccine partially diluted in each muscle 1 part of the sample to be tested from the live vaccine; 2 parts of freeze-dried diluted vaccine in each of the 5 heads of the 4th to 6th groups; the 5th ...

Embodiment 3

[0095] Embodiment 3 Application of swine fever, porcine pseudorabies and Haemophilus parasuis vaccine composition of the present invention

[0096] Trial 1: Comparative trial of vaccines

[0097] 1. Test materials The swine fever ELISA antibody detection kit was purchased from Beijing IDEXX Biotechnology Co., Ltd.; the porcine pseudorabies ELISA gB antibody detection kit was purchased from Beijing IDEXX Biotechnology Co., Ltd.

[0098] The vaccine batch number of the swine fever, porcine pseudorabies and Haemophilus parasuis vaccine of the present invention is several bottles of 20130202; the batch number of the live vaccine of swine fever produced by Guangdong Yongshun is 2012043, and the Haemophilus parasuis disease produced by Boehringer Ingelheim The batch number of live vaccine (z-1517) is 746-380, and the batch number of porcine pseudorabies live vaccine produced by Boehringer Ingelheim is 195A-23B.

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Abstract

The invention provides a vaccine composition, which comprises: immunizing amount of classical swine fever virus antigen, immunizing amount of porcine pseudorabies virus antigen and immunizing amount of Haemophilus parasuis antigen. The vaccine composition is safe to use, has controllable quality, good immune effect, and the protection rate of the virus challenge reaches 80-100%. Satisfactory immune effect can be achieved by intranasally immunizing 0-3 day-old piglets and intramuscularly immunizing 50-day-old piglets.

Description

technical field [0001] The invention relates to a vaccine composition and its preparation method and application. Background technique [0002] Classical swine fever is an acute, febrile, highly contagious infectious disease of pigs, characterized by high temperature and microvascular degeneration, which can cause systemic hemorrhage, necrosis, and infarction. In the early years, it was also known as hog cholera, which was extremely harmful to pigs and caused heavy losses to the pig industry. [0003] Haemophilus parasuis disease is multiple serositis and arthritis in pigs caused by Haemophilus parasuis (Hps), also known as Glasser's Disease. Haemophilus parasuis can affect young pigs from 2 weeks to 4 months, mainly in post-weaning and nursery stages, usually seen in pigs of 5 to 8 weeks, the incidence rate can reach 40%, and the mortality rate can reach 50% %. The main clinical symptoms were cough, dyspnea, emaciation, lameness, and rough coat; the main necropsy lesions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K47/42A61K47/32A61P31/14A61P31/22A61P31/04G01N33/569A61K39/245A61K39/187A61K39/102
Inventor 李向东田克恭张许科孙进忠
Owner PULIKE BIOLOGICAL ENG INC
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