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Swine fever vaccine heat-resistant freeze-drying protective agent, and preparation method and application thereof

A swine fever live vaccine and heat-resistant freeze-drying technology, which is applied in the field of animal virus live vaccine manufacturing, can solve the problems of long-term storage and transportation limitation of finished vaccines, poor protection function, etc. Good protective effect

Inactive Publication Date: 2014-03-12
河南后羿生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used protective agents in China are mainly composed of milk, sucrose, gelatin, etc., with simple formula and poor protective function. However, the research technology of vaccine protective agents in foreign countries is relatively confidential. Therefore, the long-term storage and transportation of finished vaccines in my country are relatively restricted big limit

Method used

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  • Swine fever vaccine heat-resistant freeze-drying protective agent, and preparation method and application thereof
  • Swine fever vaccine heat-resistant freeze-drying protective agent, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The heat-resistant lyoprotectant for swine fever live vaccine consists of the following components in weight percentage: 5% trehalose, 2% polyvinylpyrrolidone (PVP), 1% gelatin, 1% sodium glutamate, D-sorbic acid Alcohol 2%, polypeptone 4%, L-arginine 0.5%, vitamin C 0.5%, and the balance is sterilized water for injection.

[0026] The specific preparation steps of the heat-resistant lyoprotectant are:

[0027] (1) Weigh polyvinylpyrrolidone, gelatin, D-sorbitol, and polypeptone according to the weight ratio, dissolve them in a small amount of water for injection, and sterilize at 110°C for 30 minutes for later use;

[0028] (2) Weigh trehalose, sodium glutamate, L-arginine, and vitamin C according to the weight ratio, dissolve them in a small amount of water for injection, filter and sterilize them with a filter membrane with a pore size of 0.22 μm, and set aside;

[0029] (3) Mix the solutions prepared in steps (1) and (2), and add water for injection to the full amo...

Embodiment 2

[0033] The heat-resistant lyoprotectant for swine fever live vaccine consists of the following components in weight percentage: trehalose 8%, polyvinylpyrrolidone (PVP) 2.5%, gelatin 1.5%, sodium glutamate 2%, D-sorbic acid Alcohol 3.5%, polypeptone 4.5%, L-arginine 0.75%, vitamin C 0.75%, and the balance is sterilized water for injection.

[0034] The specific preparation steps of the heat-resistant lyoprotectant are:

[0035] (1) Weigh polyvinylpyrrolidone, gelatin, D-sorbitol, and polypeptone according to the weight ratio, dissolve them in a small amount of water for injection, and sterilize at 116°C for 20 minutes for later use;

[0036] (2) Weigh trehalose, sodium glutamate, L-arginine, and vitamin C according to the weight ratio, dissolve them in a small amount of water for injection, filter and sterilize them with a filter membrane with a pore size of 0.22 μm, and set aside;

[0037] (3) Mix the solutions prepared in steps (1) and (2), and add water for injection to th...

Embodiment 3

[0041] The heat-resistant lyoprotectant for swine fever live vaccine consists of the following components in weight percentage: trehalose 10%, polyvinylpyrrolidone (PVP) 3%, gelatin 2%, sodium glutamate 3%, D-sorbic acid Alcohol 5%, polypeptone 5%, L-arginine 1%, vitamin C 1%, and the balance is sterilized water for injection.

[0042] The specific preparation steps of the heat-resistant lyoprotectant are:

[0043] (1) Weigh polyvinylpyrrolidone, gelatin, D-sorbitol, and polypeptone according to the weight ratio, dissolve them in a small amount of water for injection, and sterilize at 120°C for 15 minutes for later use;

[0044] (2) Weigh trehalose, sodium glutamate, L-arginine, and vitamin C according to the weight ratio, dissolve them in a small amount of water for injection, filter and sterilize them with a filter membrane with a pore size of 0.22 μm, and set aside;

[0045] (3) Mix the solutions prepared in steps (1) and (2), and add water for injection to the full amount...

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Abstract

The invention discloses a swine fever vaccine heat-resistant freeze-drying protective agent, and a preparation method and an application thereof. The heat-resistant freeze-drying protective agent comprises the following components: trehalose, polyvinylpyrrolidone (PVP), gelatin, sodium glutamate, D-sorbic alcohol, polypeptone, L-arginine, vitamin C and water for injection and is prepared by dissolving and degerming. The heat-resistant freeze-drying protective agent is mixed with swine fever virus antigen liquid according to the volume ratio of 1:(08-1.2) and is frozen and dried after being packaged to obtain swine fever vaccine. The prepared heat-resistant freeze-drying protective agent is simple in formula, is easy to prepare, is suitable for large-scale production and has good protection efficacy on the vaccine. The prepared vaccine has the characteristics of safety and sterility, stable quality, heat resistance and long saving time. The swine fever vaccine heat-resistant freeze-drying protective agent solves the problems that the vaccine is required to be frozen at a low temperature, is not convenient to store and the like in the transportation process. Through the swine fever vaccine heat-resistant freeze-drying protective agent, the swine fever vaccine can be saved for above 30 months at a temperature of 2-8 DEG C.

Description

technical field [0001] The invention relates to a protective agent for live virus vaccines, in particular to a heat-resistant freeze-drying protective agent for live swine fever vaccines and its preparation method and application, belonging to the field of live virus vaccines for animals. Background technique [0002] Classical Swine Fever Virus (CSFV) is a highly contagious infectious disease caused by classical swine fever virus (CSFV). It has the characteristics of widespread prevalence and high incidence, and is extremely harmful to the pig industry. Classical swine fever virus is a member of the genus Pestivirus in the Flaviviridae family and is an enveloped positive-strand RNA virus. [0003] The most effective way to prevent swine fever is to vaccinate against swine fever. At present, there are three main types of vaccines for preventing swine fever on the market: milk rabbit vaccine, cell vaccine and lymphocytic vaccine. However, vaccines need to be stored in cold ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K9/19A61K39/187A61P31/14
Inventor 吴红云徐进范金红
Owner 河南后羿生物工程股份有限公司
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