Breeding method of herbicide-resistant rice two-line sterile line

A herbicide-tolerant, sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as seed impurity, and achieve the effect of solving control problems

Inactive Publication Date: 2019-01-11
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0005] For the utilization of the advantages of crop hybridization, there is a major innovative technology in the field of breeding, which is the two-line hybrid rice method. Compared with the three-line method, it can simplify the production procedure of hybrid seeds, and there is one less restorer line used in the process of seed production. Theoretically speaking, most conventional rice varieties can be used as restorer lines of two-line hybrid rice, so as to increase the freedom of hybrid rice combination selection and increase the probability of high-quality hybrid rice combination selection. However, two-line hybrid rice has the problem of impure seeds

Method used

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  • Breeding method of herbicide-resistant rice two-line sterile line
  • Breeding method of herbicide-resistant rice two-line sterile line
  • Breeding method of herbicide-resistant rice two-line sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] See figure 1 .

[0029] "CL55" is a rice mutant resistant to imidazolinone herbicides, and its resistance is controlled by a dominant gene. The homozygous herbicide-resistant gene is represented by AA, and the heterozygous herbicide-resistant gene is represented by Aa. No resistance The general rice variety is represented by aa, and the specific breeding process is as follows:

[0030] F 1 substitute seeds;

[0031] in F 1 The fertile rice plant (Aa) with excellent traits was selected as the male parent in the generation, and it was backcrossed with the two-line male sterile line PA3 as the female parent to obtain BC 1 f 1 Generation seeds (genotype has two kinds of aa, Aa), combined with field resistance screening ( figure 2 ), BC 1 f 1 The resistance genotype in the generation plants is Aa single plant planting;

[0032] Combined with field resistance screening, repeated backcrossing for 5-6 generations to obtain BC n f 1 (n=5-6), last BC n f 1 Select fe...

Embodiment 2

[0035] Example 2 Field herbicide resistance identification

[0036] The herbicide-tolerant rice two-line male sterile line PA35 obtained in Example 1 was crossed with conventional rice varieties and restorer lines.

[0037] Experimental materials: herbicide-tolerant two-line male sterile line PA35, and japonica rice hybrid combinations of PA35 and conventional rice varieties "Shanghai LPR18", "Shanghai Dao 55", and restorer lines "R65" and "R123".

[0038] Sow at the end of May, transplant in mid-June, conduct field herbicide resistance identification before transplanting, and investigate the main agronomic traits and yield of hybrid combinations at the end of October.

[0039] Field herbicide resistance identification method:

[0040] 20-25 days after rice sowing (rice 2-3 leaf stage), it was treated with imidazolinone herbicide "Dou Xerox" aqueous solution produced by Shandong Xianda Chemical Co., Ltd., and the treatment concentration was 60 grams / mu. Conventional rice var...

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Abstract

The invention discloses a breeding method of a herbicide-resistant rice two-line sterile line. According to the breeding method, an imidazolone-herbicide-resistant rice mutant is taken as a resistantgene donor parent, an excellent two-line sterile line is taken as a recurrent parent, continuous backcross is adopted so as culture the herbicide-resistant rice two-line sterile line which is excellent in agricultural properties, possesses sterility highly consistent to that of the recurrent parent and herbicide-resistant genes; the matching success rate of excellent hybridization rice combinations is increased, excellent and high yield herbicide-resistant two-line hybridization rice is cultured, the cultured herbicide-resistant two-line hybridization rice, routine rice varieties and the recurrent parent are subjected to matching, the hybridization later generations all possesses herbicide resistance, and weed control difficult problems in rice fields are solved effectively.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a method for selecting a herbicide-tolerant two-line male sterile line of rice. Background technique [0002] The occurrence of weedy rice in my country has the characteristics of wide occurrence, many types, great harm, and difficult control, which seriously affects the yield and quality of rice, and has seriously endangered my country's food security and social harmony. [0003] According to expert estimates, plus the transplanted rice, the annual loss of rice production in the country is about 3 million tons or more. In addition, farmers actually spent more than 7.2 billion yuan in labor costs for weed control rice. Due to the latent weedy rice in the paddy fields, the combined cost of prevention and loss in the whole country exceeds 10 billion yuan a year. It can be seen that the promotion of herbicide-tolerant rice varieties for the prevention and control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 白建江杨瑞芳朴钟泽方军
Owner SHANGHAI ACAD OF AGRI SCI
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