Bacterial strain and culture method and application thereof

A culturing method and strain technology are applied in the field of bacterial strains and their culturing, which can solve problems such as root-knot nematode disease, and achieve the effects of promoting growth, inhibiting the formation of root-knot nematodes, and solving the difficult problem of root-knot nematode control.

Inactive Publication Date: 2016-01-06
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the problems referred to above, the present invention obtains a strain of bacterium by systematic screening, utilizes this bacterium or the metabolite of this bacterium to process tomato seed, can induce tomato to produce the resistance to root-knot nematode incognita during seed germination and growth, thus solves the problem of root-knot nematode incognita. Knot nematodes problem

Method used

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  • Bacterial strain and culture method and application thereof
  • Bacterial strain and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Cultivation of Pseudomonas fluorescens (P.fluorescens) Sneb825

[0018] The bacterial strain of Pseudomonas fluorescens (P.fluorescens) Sneb825 was inoculated on the test tube culture medium, and the culture medium formula was beef extract peptone medium, namely ingredients: beef extract 3g, yeast extract 1g, peptone 10g, sucrose 10g, Agar 17-20g, add distilled water to 1000mL. Incubate at 28°C for 3 days to obtain test tube seeds.

[0019] Then inoculate the test tube species into a 250mL triangular flask (150mL per bottle) liquid medium, and the medium formula is: by weight percentage, yeast extract 0.1%-0.2%, peptone 0.5%-0.6%, beef extract 0.3% -0.4%, 1%-2% sucrose, the rest is distilled water, pH is 6.8-7.0, fermentation temperature is 25-28°C, shaker speed is 150r min -1 , Fermentation time 2-3d. The resulting fermentation broth or finished product of the fermentation broth can be used for direct or indirect treatment of tomato seeds. Treated tomato seeds can ...

Embodiment 2

[0021] The fermentation treatment of Pseudomonas fluorescens Sneb825 was the same as that in Example 1, except that the liquid-cultured strain was inoculated into a 1000 L fermenter for fermentation, cultured at 28° C., pH 7.0, and fermented for 3 days.

Embodiment 3

[0023] Field experiment of Pseudomonas fluorescens (P.fluorescens) Sneb825 fermentation product in controlling root-knot nematode incognita

[0024] Field effect tests were carried out in greenhouses in Liqianhu Town, Tieling City, Liaoning Province and Gaosheng Town, Panjin City, Liaoning Province. The prepared fermentation stock solution is treated with seed coating with a volume ratio of 1:40, dried under aseptic conditions, and the treated tomato seeds are sown normally, and the tomato plants can produce resistance to root-knot nematode incognita at the seedling stage. resistance, and the induced resistance effect is relatively stable, and a good control effect has been achieved.

[0025] Step 1. Preparation of test preparations

[0026] The fermentation broth of Pseudomonas fluorescens Sneb825 was prepared according to the aforementioned liquid fermentation culture method, and it was ready for use.

[0027] Step 2. Preparation of seeds for testing

[0028] Tomato varie...

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Abstract

The invention discloses a bacterial strain and a culture method and application thereof. The bacterial strain is Pseudomonas fluorescens and is collected under the number of CGMCC No.10730. A culture medium of the bacterial strain is prepared with, by mass, 0.1-0.2% of yeast extracct, 0.5-0.6% of peptone, 0.3-0.4% of beef extract, 1-2% of sucrose and the balance of distilled water. The Pseudomonas fluorescens Sneb825 or a metabolite thereof has inducing activity to tomatoes, can be used for seed treatment of the tomatoes, can promote growth of tomato plants and roots and has great inhibition effect on root-knot forming of root-knot nematodes, and the problem of prevention and control of the root-knot nematodes is solved.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a bacterial strain and its cultivation method and application. Background technique [0002] In recent years, with the continuous expansion of vegetable production in greenhouse plastic sheds in my country, agriculture has been pushed to the height of factoryization. However, with a long planting period, heavy cropping, and pests and diseases seriously affect the sustainable development of vegetable production in protected areas. Among them, root-knot nematode is common and becoming more and more serious. Root-knot nematode is a typical soil-borne disease with a wide range of hosts, including more than 3,000 species of plants. Plants such as Solanaceae, Cucurbitaceae, and Brassicaceae are particularly affected, and it has become a worldwide problem in pest control. According to statistics, the losses caused by diseases caused by root-knot nematodes are as high as tens of billio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/39
Inventor 朱晓峰陈立杰段玉玺赵丹王媛媛
Owner SHENYANG AGRI UNIV
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