Pseudomonas fragi Sned811 with function of killing root-knot nematode, metabolite and application

A technology for Pseudomonas and root-knot nematodes, which is applied in the directions of nematicides, applications, biocides, etc., to achieve good application prospects and the effect of strong poisoning effect

Active Publication Date: 2018-02-09
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And utilize Pseudomonas berry or the metabolite of Pseudomonas berry to process tomato root, the method for preventing and treating root-knot nematode incognita has not yet been reported

Method used

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  • Pseudomonas fragi Sned811 with function of killing root-knot nematode, metabolite and application
  • Pseudomonas fragi Sned811 with function of killing root-knot nematode, metabolite and application
  • Pseudomonas fragi Sned811 with function of killing root-knot nematode, metabolite and application

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Experimental program
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Embodiment 1

[0031] In this embodiment, a root-knot nematode metabolite prepared by Pseudomonas berry Sned811, the preparation method is as follows:

[0032] After activating the test tube strain of Pseudomonas berry Sned811, inoculate it into the test tube culture medium for seed culture. The formula of the test tube culture medium is beef extract peptone medium, that is, the ingredients: beef extract 3g, yeast extract 1g, Peptone 10g, sucrose 10g, agar 17g, add distilled water to 1000mL. The seed culture conditions are as follows: culture at 28°C for 3 days to obtain a test tube seed solution.

[0033] Then the obtained seed solution was inoculated in a 250mL Erlenmeyer flask (150mL per bottle) liquid fermentation medium to carry out fermentation culture to obtain root-knot nematode metabolites. The formula of the liquid fermentation medium is: in terms of mass percentage, 0.1% of yeast extract, 0.5% of peptone, 0.3% of beef extract, 1% of sucrose, the rest is distilled water, and the p...

Embodiment 2

[0035] In this embodiment, a root-knot nematode metabolite prepared by Pseudomonas berry Sned811, the preparation method is as follows:

[0036] After activating the test tube strain of Pseudomonas berry Sned811, inoculate it into the test tube culture medium for seed culture. The formula of the test tube culture medium is beef extract peptone medium, that is, the ingredients: beef extract 3g, yeast extract 1g, Peptone 10g, sucrose 10g, agar 20g, add distilled water to 1000mL. The seed culture conditions are as follows: culture at 28°C for 3 days to obtain a test tube seed solution.

[0037] Then the obtained seed solution was inoculated in a 250mL Erlenmeyer flask (150mL per bottle) liquid fermentation medium to carry out fermentation culture to obtain root-knot nematode metabolites. The formula of the liquid fermentation medium is: in terms of mass percentage, 0.2% of yeast extract, 0.6% of peptone, 0.4% of beef extract, 2% of sucrose, the rest is distilled water, and the p...

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Abstract

The invention discloses pseudomonas fragi Sned811 with a function of killing tomato southern root-knot nematode with poison. A classification name of the pseudomonas fragi Sned811 is Pseudomonas fragi, the pseudomonas fragi Sned811 is preserved in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms on April 12th, 2017, and a preservation number of the pseudomonas fragi Sned811 is CGMCC No.14018. The pseudomonas fragi Sned811 has the advantages that biological preparations with strong effects of killing the tomato southern root-knot nematode with poison can be developed from a metabolite obtained after a strain of the pseudomonas fragi Sned811 is cultured according to conventional liquid fermentation culture, the strong effects of killing the tomato southern root-knot nematode with poison can be realized, obvious effects of inhibiting hatching of oocysts of the tomato southern root-knot nematode can be realized, and accordingly the pseudomonas fragi Sned811 is a biological control bacterium with great potential and has an excellent application prospect in the aspect of controlling harm on crops due to root-knot nematode.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to Pseudomonas berry Sned811 for killing root-knot nematodes, metabolites and applications. Background technique [0002] Root-knot nematode (Meloidogyne incognita) causes significant yield loss in vegetables around the world, is difficult to control, and makes plants more susceptible to complex infections of other fungi and bacteria. Effective, highly toxic and high-residue chemical nematicides pose potential threats to soil ecosystems, groundwater, and human health. Currently, dissatisfaction with chemical nematicides has spurred research into pollution-free alternative control strategies. The development of alternatives to highly toxic and high residue chemical nematicides is one of the major future issues. [0003] There are many types and quantities of soil microorganisms, which are closely related to soil nematodes and crop roots. Among them are man...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/38
CPCA01N63/10C12N1/20C12N1/205C12R2001/38
Inventor 陈立杰赵惠段玉玺赵丹朱晓峰王媛媛
Owner SHENYANG AGRI UNIV
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