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Egg yolk antibody for preventing and treating novel duck reovirus and preparation method of egg yolk antibody

A technology for reovirus and egg yolk antibody is applied in the field of preventing and treating egg yolk antibody of new duck reovirus and its preparation, and can solve the problems of restricting the development and application of egg yolk antibody of new duck reovirus, inconvenient use and the like, Achieving good commercial development prospects, good safety, and excellent immunogenicity

Active Publication Date: 2018-10-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since this disease is reported for the first time in my country, there is no commercialized vaccine and antibody on the market, and the egg yolk antibody used for the emergency prevention and early infection treatment of this new type of duck reovirus has not been reported; Containing a large amount of lipids brings a lot of inconvenience to use, which also restricts the development and application of the yolk antibody for preventing and treating this novel duck reovirus

Method used

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  • Egg yolk antibody for preventing and treating novel duck reovirus and preparation method of egg yolk antibody
  • Egg yolk antibody for preventing and treating novel duck reovirus and preparation method of egg yolk antibody

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Example 1: Discovery and identification of a new duck reovirus

[0060] 1. Virus isolation:

[0061] (1) For ducks with arthritis that were clinically identified as positive by PCR reaction, the swollen tendon tissue was collected by autopsy and placed in a 15 mL centrifuge tube, and 5 times the volume of serum-free DMEM medium was added, and the homogenization was repeated freezing and thawing. 3 times, after each thawing, shake on the shaker for 1-2 minutes, and then perform the next freeze-thaw; after freezing and thawing, centrifuge the 15mL centrifuge tube containing the sample in the centrifuge at 4000rpm for 5min; take the supernatant, 0.22μm micropores After the filter membrane is filtered, spare;

[0062] (2) According to the proliferation characteristics of reo, chicken liver cancer cells (LMH) were selected for virus isolation. According to the conventional cell culture method, wait for 25cm 2 When the cells in the cell flask cover the monolayer, aspirate the cult...

Embodiment 2

[0078] Example 2: Preparation of inactivated vaccine

[0079] (1) Virus propagation procedure: The new duck reovirus strain isolated in Example 1 was inoculated with healthy SPF chicken embryos aged 9 days at a dose of 0.2ml / piece, discarded the dead embryos within 24h, and collected dead chickens within 24 to 120h Embryo allantoic fluid, obtain virus fluid, store at -20°C.

[0080] (2) Concentration of virus liquid: The harvested virus liquid is concentrated to 1 / 3 of the original volume with an ultrafiltration concentrator under the condition of 2~8℃, and the sterility test is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia" to grow aseptically , And keep samples for drug price testing. Virus content per 0.1ml of virus liquid should be ≥10 5.0 ELD 50 , The concentrated allantoic fluid is then inactivated.

[0081] (3) Inactivation: Lead the concentrated virus liquid into an inactivation tank, and add 10% formaldehyde solution to make the fin...

Embodiment 3

[0089] Example 3: Preparation of New Type Duck Reovirus Yolk Antibody

[0090] 1. Use inactivated vaccine to immunize laying hens:

[0091] The new inactivated reovirus vaccine prepared in Example 2 was used to immunize laying hens. For the first immunization, each chicken was immunized with 2.0ml of inactivated vaccine subcutaneously in the neck, and 14 days later for the second immunization, each chicken was injected with 2.0ml subcutaneously in the neck Inactivated vaccine, the third immunization is carried out 14 days after the second immunization, 2.0ml of inactivated vaccine is injected subcutaneously in the neck of each chicken, and booster immunization is carried out 14 days after the third immunization, and 2.0ml of inactivated vaccine is injected subcutaneously in the neck of each chicken. 14 days after the booster immunization, the yolk should be collected to determine the titer of the new reovirus neutralizing antibody ≥ 1:1024.

[0092] 2. Production of yolk antibody:

...

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Abstract

The invention discloses an egg yolk antibody for preventing and treating novel duck reoviruses and a preparation method of the egg yolk antibody. The egg yolk antibody comprises an anti-duck reoviru strain antibody, and a duck reoviru strain has a preservation number of CCTCC NO: V201818. The egg yolk antibody disclosed by the invention is good in safety, free of partial or whole-body adverse reaction caused by the egg yolk antibody, is capable of effectively preventing and / or treating infection of the novel duck reoviruses, and has a very good commercial development prospect.

Description

Technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a yolk antibody for preventing and treating a new duck reovirus and a preparation method thereof. Background technique [0002] The genome of reovirus is a double-stranded segmented RNA. In 1978, the International Commission for Virus Isolation (ICTV) designated it as the Reoviridae family (Reoviridae), including the genus Reoviridae, Circovirus and Rot Virus genus. Avian reovirus (Avian reovirus) was isolated in the respiratory tract of chickens for the first time. After infection with susceptible chickens, it can show symptoms such as tendinitis, synovitis and respiratory diseases. ARV can infect chickens, turkeys, ducks, geese, parrots, pigeons and other birds. It can cause viral arthritis and tenosynovitis in chickens. It is also the cause of other diseases, including myocarditis, pericarditis, pericardial effusion, hepatitis, and thymic atrophy. , Respira...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P31/14
CPCA61K2039/505C07K16/10C07K2317/11
Inventor 唐熠刁有祥高斌
Owner SHANDONG AGRICULTURAL UNIVERSITY
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