Recombinant UL51 protein-based method for detecting duck plague virus antibody

Abstract

The invention relates to the field of animal medicine, in particular to a method for detecting duck plague virus antibody. The method particularly comprises the steps of the preparation of solid phase antigen, primary antibody combination, secondary antibody combination, developing, detection, judgment and the like. The method is an indirect enzyme-linked immuno sorbent assay (ELISA) method established based on purified recombinant UL51 protein, which has good specificity, and when duck hepatitis virus (DHV), Riemerella anatipestifer (RA), duck Escherichia coli (E.coli) are detected, the detection results are all positive; intra batch or batch-to-batch repeated tests show that variation coefficients are all less than 10%, and duck plague virus (DPV) vaccine immunized duck positive sera diluted at ratio of 1:3200 can be detected.

Description

technical field [0001] The invention relates to the field of animal medicine, in particular to a method for detecting duck plague virus antibody based on recombinant UL51 protein. Background technique [0002] Duck plague (duckplague, DP) is caused by DPV in the Herpesviridae family and is a highly fatal infectious disease common in waterfowl such as ducks, geese, and swans. It is directly related to the sustainable and stable development of the waterfowl breeding industry. Clinical and laboratory tests have confirmed that DPV attenuated vaccine is an effective biological agent for the prevention and control of DP, and the monitoring of DPV-specific antibodies is the key to evaluating the immune effect of DPV attenuated vaccine and formulating a reasonable immunization program. At present, the methods used to detect DPV antibodies mainly include neutralization test (neutralization test, NT), enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA), agar ...

AI Technical Summary

Problems solved by technology

The classic method is the serum neutralization test, but its sensitivity is not ideal, and it is time-consuming and laborious, so it is not suitable for the detection of large quantities of serum samples

ELISA has the characteristics of strong specificity, high sensitivity, fast accuracy, and easy operation. It is an effective means for early rapid diagnosis of DPV infection and monitoring of immune antibodies; however, the complexity and purity of DPV whole virus purification methods are not ideal. Large-scale application of ELISA method (DPV-ELISA) coated with whole virus

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