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Duck plague yolk antibody freeze-dried powder and preparation method thereof

A technology of egg yolk antibody and freeze-dried powder, which is applied in the direction of antibodies, freeze-dried transportation, antiviral agents, etc., can solve the problems of time-consuming and labor-intensive, and achieve the effects of long storage time, slow reduction of potency and good preventive effect

Inactive Publication Date: 2012-10-10
ZHENGZHOU HOUYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in actual production, animal blood collection is not only a great stress on the animal itself, but also time-consuming and labor-intensive. Large-scale production is impractical, and it is much more convenient to use eggs to produce antibodies.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1. The freeze-dried powder of duck plague yolk antibody of the present embodiment comprises the following components: duck plague yolk antibody 90.79%, formaldehyde 0.2%, thimerosal 0.01%, 3% glucose, 1% sorbitol, 2% glycine, 2% Mannitol, octanoic acid 1.0%, the above reagents are analytically pure.

[0022] The preparation method of the duck plague egg yolk antibody freeze-dried powder of the present embodiment, the specific steps are:

[0023] 1) Eggs obtained from immune healthy duck flocks: immunized with 1.5 mL of duck plague inactivated vaccine (containing 10 3.2 ELD 50 / 0.1Ml), the healthy duck flock was immunized with duck plague inactivated vaccine for the first time, with an interval of 7 days between each immunization, and then doubled the amount of immunization, and immunized three times. After the second immunization, the antibody titer was detected to make the antibody When the titer reaches 1:256, start to collect eggs, and the collected eggs...

Embodiment 2

[0048] Embodiment 2. The freeze-dried powder of duck plague yolk antibody of the present embodiment comprises the following components: duck plague yolk antibody 88.935%, formaldehyde 0.25%, thimerosal 0.015%, 3.5% glucose, 1.5% sorbitol, 2.5% glycine, 2.5% Mannitol, caprylic acid 0.8%.

[0049] The preparation method of the duck plague high-free egg yolk antibody freeze-dried powder of this embodiment, the specific steps are:

[0050] 1) Choose self-isolated virus strains to prepare vaccines. The virus samples are mainly collected from duck plague samples in Fujian. After aseptic collection of cases with obvious clinical symptoms, the collected samples are ground, frozen and thawed 3 times, and 15000rpm / min high speed The supernatant was collected by centrifugation, and the supernatant was inoculated into chicken embryo sacs to obtain the FJ strain of the virus strain. The primers were designed according to the UL28 gene sequence published by GenBank accession number EF643557...

Embodiment 3

[0076] Embodiment 3. The freeze-dried powder of duck plague yolk antibody of the present embodiment comprises the following components: duck plague yolk antibody 87.78%, formaldehyde 0.3%, thimerosal 0.02%, glucose 4%, sorbitol 2%, glycine 3%, mannitol 2%, caprylic acid 0.9%.

[0077] The preparation method of the duck plague high immunity yolk antibody freeze-dried powder of this embodiment can be prepared by the conventional freeze-dried powder preparation method.

[0078] In order to detect the change of the antibody titer when the yolk antibody is placed at room temperature, so as to determine the shelf life of the product, the following tests were done: the product was stored in a 37°C incubator for 24 months, respectively for 1 week and 2 days. Weeks, January, February, April, August, December, 16 months, 20 months, and 24 months later, use the agar diffusion (AGP) method to measure the changes in the yolk antibody titer of samples taken at each time point, and the resul...

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Abstract

The invention relates to duck plague yolk antibody freeze-dried powder, which comprises the following components in percentage by weight: 87 to 90 percent of duck plague yolk antibody, 0.8 to 1.0 percent of octanoic acid, 0.2 to 0.3 percent of formaldehyde, 0.01 to 0.02 percent of thimerosal, 3 to 4 percent of glucose, 1 to 2 percent of sorbitol, 2 to 3 percent of glycine and 2 to 3 percent of mannitol. The freeze-dried powder has the advantages of long storage time, quick dissolution, high antibody valence and high bioactivity, and the purity of the antibody of the freeze-dried powder is higher than that of the conventional product in market. The invention also discloses a preparation method for the duck plague yolk antibody freeze-dried powder. The preparation method comprises the following steps of: separating strains to prepare vaccines, immunizing healthy ducks to obtain high-immunity eggs, performing sterilization, acidification, octanoic acid treatment, refining, extraction, purification, preparation, freeze drying and the like on the high-immunity eggs, and thus obtaining the duck plague high-immunity yolk antibody freeze-dried powder. According to the preparation method, yolk liquid is heated by using the temperature of pasteurization during acidification and water treatment, so that a sterilization effect is achieved, and the recovery rate of the antibody and the clarity of the solution during acidification can be improved at the same time; and the prepared yolk antibody has the advantages of high bioactivity, long storage time, retarded valence reducing speed and the like.

Description

technical field [0001] The invention belongs to the technical field of biological products for poultry, in particular to a duck plague egg yolk antibody freeze-dried powder and a preparation method thereof. Background technique [0002] Duck plape, also known as duck virus enteritis (DVE), is an acute, febrile, septic infectious disease of ducks, geese and other Anseriformes. The disease is characterized by widespread prevalence, rapid spread, and high morbidity and mortality. As early as 1923, Baudet first discovered the disease in the Netherlands, until 1940, Bos first proposed the name of duck plague, and confirmed that it was a new virus disease different from chicken plague. Afterwards, there were reports of the occurrence of this disease in various countries in Europe and the United States. The official report of the prevalence of duck plague in my country was first proposed by Huang Yinxian in Guangdong in 1957. Subsequently, it was discovered in Wuhan, Shanghai, Zh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/19A61P31/14
CPCY02A50/30
Inventor 吴红云徐进徐荣君郭俊清
Owner ZHENGZHOU HOUYI PHARMA
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