Establishment method and application of H9 subtype avian influenza recombined duck enteritis virus rDEVdeltagE-H9 strain

A technology of avian influenza duck enteritis and duck enteritis virus, which is applied in the field of virology and veterinary biological products, can solve the problems of ducklings with respiratory symptoms, immune suppression, and the increase of sand shell eggs

Pending Publication Date: 2016-01-20
CHINA INST OF VETERINARY DRUG CONTROL
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AI Technical Summary

Problems solved by technology

[0004] Since the isolation of H9N2 subtype avian influenza virus in a chicken farm in Guangdong, my country in 1994, this subtype virus has been widely prevalent in chickens, ducks, geese and other poultry in my country, and has been on the rise, and its pathogenicity to duck

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  • Establishment method and application of H9 subtype avian influenza recombined duck enteritis virus rDEVdeltagE-H9 strain
  • Establishment method and application of H9 subtype avian influenza recombined duck enteritis virus rDEVdeltagE-H9 strain
  • Establishment method and application of H9 subtype avian influenza recombined duck enteritis virus rDEVdeltagE-H9 strain

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Embodiment Construction

[0014] 1. Virus seeds for production

[0015] Its production virus seed of vaccine described in the present invention is the recombinant duck enteritis virus (rDEVΔgE-H9 strain) that expresses H9 subtype avian influenza virus HA gene, and this virus construction method is as follows:

[0016] (1) carrier

[0017] The pMD-18T vector, used for cloning, sequencing and construction of transfer vectors, was purchased from Bao Biological Engineering (Dalian) Co., Ltd. The GFP reporter gene vector pT-GFP-gpt containing CMV promoter, polyA (see figure 1 ), constructed and preserved by the inventor's laboratory.

[0018] (2) Primer design

[0019] The following primers were designed and synthesized according to the DEV sequence published on GenBank:

[0020] Table 1 The relevant primer information involved in the construction of the present invention

[0021]

[0022] (3) Construction of duck enteritis virus rDEVΔgE-GFP strain containing GFP marker

[0023] 1) Construction of ...

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Abstract

The invention relates to a recombined duck enteritis virus for expressing an exogenous gene, and relates to an establishment method and application of the recombined duck enteritis virus. Particularly, a recombined duck enteritis virus carrier rDEVdeltagE-GFP which is lack of a gE gene and carries a green fluorescence marker is established, the recombined duck enteritis virus which can express the exogenous gene can be obtained through the homologous recombination method, and the foundation is laid for establishing a novel recombined vaccine resisting duck plague and other pathogeny with the duck enteritis virus as the carrier; the duck enteritis virus rDEVdeltagE-H9 strain expressing the H9 subtype avian influenza virus hemagglutinin (HA) is established. Application of the recombined duck enteritis virus (rDEVdeltagE-H9) for expressing the H9 subtype avian influenza virus hemagglutinin (HA) is used for preparing the vaccine for treating duck plague and the H9 subtype avian influenza.

Description

technical field [0001] The invention relates to the construction and application of H9 subtype avian influenza recombinant duck enteritis virus rDEVΔgE-H9 strain and belongs to the fields of virology and veterinary biological products. Background technique [0002] Duck enteritis virus (DEV), also known as duck plague virus, can infect ducks, geese and other birds of Anseriformes, causing vascular damage, tissue bleeding, gastrointestinal mucosal damage, lymphoid organ damage and parenchymal organ degeneration An acute, contact, septic infectious disease - duck viral enteritis (Duckviralenteritis, DVE), or duck plague (Duckplague, DP) (Sandhuan and Metwally, 2008). Since it was first discovered in 1923 (Baudet, 1923), the disease has successively occurred in countries such as France, India, Belgium, the United Kingdom, Thailand, and Canada (Yin Zhen and Liu Jinghua, 1997), and the scope of the epidemic and the types of infected poultry have gradually expanded. According to ...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/44C12N15/869A61K39/295A61K39/245A61K39/145A61P31/16A61P31/22
CPCY02A50/30
Inventor 杨承槐陈光华李俊平孙莹李岭李启红曹明慧李慧姣
Owner CHINA INST OF VETERINARY DRUG CONTROL
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