Duplex egg yolk antibody freeze-drying powder for duck virus hepatitis and duck plague and preparation method thereof

Abstract

The invention relates to duplex egg yolk antibody freeze-drying powder for duck virus hepatitis and duck plague, which comprises each following component according to weight percent: 30 to 60 percent of egg yolk antibody for duck plague, 30 to 55 percent of egg yolk antibody for duck virus hepatitis, 0.2 to 0.3 percent of formaldehyde, 0.01 to 0.02 percent of thiomersalate, 4 to 5 percent of glucose, 1 to 2 percent of sorbitol, 2 to 3 percent of glycocoll and 2 to 3 percent of mannitol. The invention discloses a preparation method of the duplex egg yolk antibody freeze-drying powder for the duck virus hepatitis and the duck plague simultaneously, which comprises the following steps of: separating a strain, preparing a vaccine, immunizing a healthy duck flock, preparing eggs, carrying out acidizing treatment, octoate treatment and refining, extracting and purifying treatment after separating egg yolks, preparing and freeze-drying so as to obtain the duplex egg yolk antibody freeze-drying powder for the duck virus hepatitis and the duck plague. According to the duplex egg yolk antibody freeze-drying powder for the duck virus hepatitis and the duck plague and the preparation method thereof, viruses sampled in regions with high morbidity are separated, the virus strain with wider coverage range is sieved, the vaccine is prepared, the cold / hot acidizing treatment of an egg yolk solution is also carried out, the antibody loss is reduced, and the obtained egg yolk antibody freeze-drying powder has high recovery rate and high purity, can dissolve and act quickly and has no clinical side effect.

Description

technical field [0001] The invention belongs to the technical field of biological products for poultry, and relates to a freeze-dried powder of duck viral hepatitis and duck plague dual-linked yolk antibody and a preparation method thereof. Background technique [0002] Duck viral hepatitis, referred to as duck hepatitis, is one of the most serious diseases that harm ducklings. It mainly harms ducklings less than 20 days old. The younger the ducklings, the higher the mortality rate, and the highest mortality rate can reach 90%-95. %, and all antibiotics are ineffective against this disease. Duck viral hepatitis was discovered in my country as early as 1958, and the disease became prevalent in my country again in the early 1980s. Since then, epidemics have occurred one after another in various places, and the epidemic can be controlled by the immunization of duck viral hepatitis I type attenuated vaccine; since 1997 , the disease is more serious in some areas, and its epidemi...

AI Technical Summary

Problems solved by technology

In addition, in actual production, animal blood collection is not only a great stress on the animal itself, but also time-consuming and labor-intensive. Large-scale production is impractical, and it is much more convenient to use eggs to produce antibodies.