Helicobacter pylori fluorescent staining reagent

Abstract

The invention discloses a helicobacter pylori fluorescent staining reagent and belongs to the technical field of medical detection reagents. The helicobacter pylori fluorescent staining reagent comprises, by weight, 0.5-2 parts of acridine orange, 0.5-1.5 parts of acetic acid, 1-5 parts of sodium acetate, 1-5 parts of calcium chloride, 1-3 parts of glycerol, and 1-5 parts of a counterstain. The helicobacter pylori fluorescent staining reagent can stain helicobacter pylori orange red by acridine orange fluorescent staining, so that the helicobacter pylori can be clearly seen under a fluorescence microscope with high contrast between the bacteria and the background, and significantly higher sensitivity than that of conventional straining, the staining can be completed with one step, and a result can be obtained within about 1 minute, thus the helicobacter pylori fluorescent staining reagent is fast and efficient. In addition, acetic acid and sodium acetate are added so that acridine orange has strong binding ability with cell DNA and RNA, so as to achieve good staining effect, and the counterstain has a function of suppressing the background and reducing background stray light. The staining reagent of the invention is more stable, bright in color contrast, and convenient for observation.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a fluorescent staining reagent for Helicobacter pylori applied to paraffin sections of gastric mucosa. Background technique [0002] Helicobacter pylori is a spiral-shaped, micro-anaerobic bacteria that requires very harsh growth conditions. In 1983, it was successfully isolated from the gastric mucosal biopsy tissue of patients with chronic active gastritis for the first time. It is the only microbial species known to be able to survive in the human stomach. On October 27, 2017, the list of carcinogens released by the International Agency for Research on Cancer of the World Health Organization was initially compiled for reference. Helicobacter pylori (infection) was included in the list of carcinogens. [0003] Studies have pointed out that Helicobacter pylori lives in the pyloric part of the human stomach and is one of the most common bacterial pathogens. Half of the world's populatio...

AI Technical Summary

Problems solved by technology

However, these staining methods have obvious disadvantages. The main reason is that the operation is cumbersome, all of which are multi-step operations, and the detection time is long; non-specific staining, the sensitivity to Helicobacter pylori staining is poor, the contrast after background coloring is small, and the bacteria are difficult to find. When it is small, it is easy to cause missed diagnosis

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