Dyeing liquid as well as dyeing method and uses thereof
A dyeing solution and solution technology, applied in biochemical equipment and methods, microbial determination/inspection, fluorescence/phosphorescence, etc., can solve the problems of too many equipment, poor effect, long time, etc., and achieve the effect of good reference
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Embodiment 1
[0032] Embodiment one, dyeing solution preparation method
[0033] 1. Dye source: acridine orange and propidium iodide are packaged by Sigma.
[0034] 2. Preparation method: use 0.38% NaCl solution to prepare 0.2% acridine orange solution and 0.013% propidium iodide solution, acridine orange solution and propidium iodide solution are mixed in a ratio of 3:1, 0.22 μm mixed cellulose ester Filter through a microporous membrane to remove impurities and bacteria in the dye solution, and set aside. The use of 0.38% NaCl solution to prepare the dye can make the stained smear easier to observe, and the effect of the microscopic image is better.
Embodiment 2
[0035] Embodiment two, dyeing solution preparation method
[0036] 1. Dye source: acridine orange and propidium iodide are packaged by Sigma.
[0037] 2. Preparation method: prepare 0.3% acridine orange solution and 0.0067% propidium iodide solution with double distilled water, mix acridine orange solution and propidium iodide solution in a ratio of 3:1, mix cellulose ester microparticles of 0.22 μm Filter through a pore filter to remove impurities and bacteria in the dye solution, and set aside.
Embodiment 3
[0038] Embodiment three, dyeing solution preparation method
[0039] 1. Dye source: acridine orange and propidium iodide are packaged by Sigma.
[0040] 2. Preparation method: prepare 0.05% acridine orange solution and 0.026% propidium iodide solution with double distilled water, mix acridine orange solution and propidium iodide solution in a ratio of 3:1, mix 0.22 μm cellulose ester micro Filter through a pore filter to remove impurities and bacteria in the dye solution, and set aside.
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