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Dyeing liquid as well as dyeing method and uses thereof

A technology of staining solution and solution, which is applied in the fields of biochemical equipment and methods, measurement/testing of microorganisms, fluorescence/phosphorescence, etc., to achieve a good reference effect

Inactive Publication Date: 2012-01-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, the method for discriminating spore viability also has the method of adopting spore germination identification, but because the spore filament is very small (about 120~140 μm in length and about 0.1 μm in width) ), even with a phase-contrast microscope, the effect is not good, and it is even more difficult to observe the exact number of live spores or the degree of viability of the spores
However, the staining methods used to identify cell viability, such as trypan blue staining and methylene blue staining, etc., due to the special structure of the exine spore wall, such as thick skin, etc., the staining effect is not ideal

Method used

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  • Dyeing liquid as well as dyeing method and uses thereof
  • Dyeing liquid as well as dyeing method and uses thereof
  • Dyeing liquid as well as dyeing method and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Embodiment one, dyeing solution preparation method

[0034] 1. Dye source: acridine orange and propidium iodide are packaged by Sigma.

[0035] 2. Preparation method: use 0.38% NaCl solution to prepare 0.2% acridine orange solution and 0.013% propidium iodide solution, mix acridine orange solution and propidium iodide solution at a ratio of 3:1, and mix cellulose ester at 0.22 μm Filter through a microporous membrane to remove impurities and bacteria in the dye solution, and set aside. The use of 0.38% NaCl solution to prepare the dye can make the stained smear easier to observe, and the effect of the microscopic image is better.

Embodiment 2

[0036] Embodiment two, dyeing solution preparation method

[0037] 1. Dye source: acridine orange and propidium iodide are packaged by Sigma.

[0038] 2. Preparation method: use double distilled water to prepare 0.3% acridine orange solution and 0.0067% propidium iodide solution, mix acridine orange solution and propidium iodide solution at a ratio of 3:1, and mix 0.22 μm cellulose ester micro Filter through a pore filter to remove impurities and bacteria in the dye solution, and set aside.

Embodiment 3

[0039] Embodiment three, dyeing solution preparation method

[0040] 1. Dye source: acridine orange and propidium iodide are packaged by Sigma.

[0041] 2. Preparation method: use double-distilled water to prepare 0.05% acridine orange solution and 0.026% propidium iodide solution, mix acridine orange solution and propidium iodide solution at a ratio of 3:1, and mix 0.22 μm cellulose ester micro Filter through a pore filter to remove impurities and bacteria in the dye solution, and set aside.

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Abstract

The invention relates to a dyeing liquor used to discriminate the protozoan cell activity, a method to discriminate the protozoan cell activity and the application of the method to discriminate activity of nosema bombycis, which is characterized in that: the dyeing liquor comprises the following components: three acridine oranges with the concentration of 0.05% to 0.3% and one propidium iodide with the concentration of 0.0067% to 0.026%; the acridine oranges and the propidium iodide are prepared by NaCl solution with the concentration of 0.38%; the method to discriminate the protozoan cell activity comprises adequate material choosing, dyeing liquor preparing, dyeing, microscopic examination and protozoan cell activity discriminating based on the color change of microscopic examination results. The dyeing liquor used to discriminate the protozoan cell activity has the advantages that: the spore activity of nosema bombycis can be discriminated quickly, sensitively, accurately and visually; the deficiency of the prior art is overcome.

Description

technical field [0001] The invention belongs to the field of cell viability detection, and particularly relates to a staining solution for identifying protozoan cell viability, a method for using the staining solution to identify protozoa cell viability, and an application of the method in identifying the viability of microsporidia silkworm. Background technique [0002] Sericulture is a traditional industry in my country with a long history. While meeting the needs of people's material life, it has also become a bond of friendly exchanges between our people and neighboring countries. The famous Silk Road originated from silkworm production. Today, sericulture has created a large amount of foreign exchange and wealth for the country while enriching people's material life and increasing farmers' income. However, for a long time, sericulture has been plagued by the silkworm microparticle disease. Bombyx mori microparticle disease is a serious infectious disease caused by Nos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04G01N21/64
Inventor 廖富蘋王正勇林健荣
Owner SOUTH CHINA AGRI UNIV
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