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Nanometer golden rod used for detecting bovine serum albumin and preparation method and application thereof

A technology of bovine serum albumin and nano-gold rods, applied in nanotechnology, nanotechnology, nanotechnology, etc. for materials and surface science, to achieve rapid detection, uniform appearance, and good linearity

Inactive Publication Date: 2015-06-17
JIANGSU DONGRUN MEDICAL SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no method to coat gold nanorods with acridine orange doped mesoporous silicon for the detection of bovine serum albumin

Method used

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  • Nanometer golden rod used for detecting bovine serum albumin and preparation method and application thereof
  • Nanometer golden rod used for detecting bovine serum albumin and preparation method and application thereof

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with specific examples.

[0020] Preparation of gold seeds: NaBH prepared with 0.24 mL of 0.1 mol / L ice water 4 Dilute to 0.4mL, then add to 2mL 0.5mmol / L HAuCl 4 Into the solution mixed with 2mL of 0.2mol / L CTAB, stirred at high speed for 2min, and then allowed to stand for 2h. The preparation temperature of gold seeds was controlled at 25°C.

[0021] Preparation of gold rods: Add 0.56g CTAB and 0.0987g sodium oleate to 20mL water and stir to dissolve at 50°C, then cool to 30°C, add 1.92mL of 4mmol / L AgNO 3 After standing still for 15min, add 20mL of 1mmol / L HAuCl 4 Stir at a medium speed for 90 minutes. After it becomes colorless, add 0.168 mL of concentrated hydrochloric acid and stir for 15 minutes. Then add 0.100 mL of 0.064 mol / L ascorbic acid and stir at a high speed for 30 seconds. Finally, inject 64 μL of gold seeds and let it grow overnight. to obtain gold nanorods. The growth temperatur...

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Abstract

The invention discloses a nanometer golden rod which is wrapped in fluorescent dye, provided with a core-shell structure and used for detecting bovine serum albumin and a preparation method and application thereof. First, HAuCl4 in a CTAB solution at a certain concentration is reduced through NaBH4 so that gold seeds can be obtained; then silver nitrate is added in a solution containing CTAB and sodium oleate, and HAuCl4 is added after standing; ascorbic acid is added, finally, the gold seeds which are obtained before are added, mixtures grow overnight, and the gold nanorod which is uniform in shape is finally obtained; acridine orange dye is added in a gold nanorod solution at a certain concentration under the condition of violent stirring, the pH value is adjusted to 10 through a NaOH solution, then, a mixed solution is slowly stirred, a methanol solution of tetraethyl silicate is added many times in the mode that a small number of methanol solution of the tetraethyl silicate is added each time, finally the solution is stirred for 24 hours so that the gold nanorod of the core-shell structure can be obtained. The composite nanometer materials obtained through the synthesis method are good in water solubility and biocompatibility and uniform in shape, when the bovine serum albumin is detected, signals are stable, linearity is good, and detection is fast and easy.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a nano-gold rod for detecting bovine serum albumin, a preparation method and application thereof. Background technique [0002] In the past ten years, the most mature method for the synthesis of gold nanorods has been the seed growth method using cetyltrimethylammonium bromide (CTAB) as a surfactant [see 1. Nikoobakht B., El- Sayed, M.A., Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method. Chem. Mater. 2003, 15, 1957-1962.2. Sau T.K., Murphy C.J., Seeded high yield synthesis of short Au nanorods in aqueous solution . Langmuir. 2004, 20, 6414-6420]. An improved synthesis method that appeared in 2013 corresponds to a uniform shape of gold nanorods, and the conditions are easy to control [see Ye X., Zheng C., Chen J., Gao Y., et al. Using binary surfactant mixtures to simultaneously improve the dimensional tenability and monodispersity in ...

Claims

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Application Information

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IPC IPC(8): B22F1/02B22F9/24B82Y40/00B82Y30/00G01N21/64
Inventor 王伟刘国良宣成磊
Owner JIANGSU DONGRUN MEDICAL SCI & TECH
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