Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preprocessing raw milk

A technology of raw milk and weight, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., and can solve problems such as somatic cell detection interference

Inactive Publication Date: 2011-05-25
CHINESE ACAD OF AGRI MECHANIZATION SCI
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fat globules, protein micelles, etc. contained in it have caused great interference to the detection of somatic cells, and it is impossible to directly identify and analyze

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 to the pretreatment of raw milk

[0032] 1. Dilute 10 ml of raw milk (provided by Beijing Xiaowangzhuang Dairy Farm) with deionized water, and the weight of the diluted sample is twice the weight of the raw milk before dilution;

[0033] 2. The diluted sample was hydrolyzed for 5 minutes according to the ratio of adding 1 milliliter of subtilisin solution (produced by Shanghai Lianguan Biochemical Co., Ltd., with a concentration of 8 to 9.5% (weight)) for every 3 milliliters of the diluted sample , the temperature of the hydrolysis system is 22°C;

[0034]3. Use the acridine orange solution (produced by Shanghai Ruji Biotechnology Development Co., Ltd.) that is 5% (weight) to dye the sample after hydrolysis, and the acridine orange solution is according to the volume of the sample after hydrolysis: acridine orange solution The volume was added at a volume ratio of 100:1; the staining time was 7.5 minutes.

[0035] The obtained samples met the computer ide...

Embodiment 2

[0036] The artificial microscope detection of embodiment 2 somatic cells

[0037] The raw milk samples of the same batch as in Example 1 were detected by a manual microscope, and the specific steps are as follows:

[0038] 1) Prepare melanin staining solution: add 54ml of ethanol and 40ml of tetrachloroethane into a round bottom flask, heat to 70°C, add 1.2g of melanin, shake vigorously to dissolve it rapidly, after cooling, slowly add 6ml of glacial acetic acid , and then plug the sealing plug, put it in a brown bottle, and store it in a dry, cool and dark place for later use;

[0039] 2) Cleaning of the glass slide: the hydrochloric acid with a concentration of 4-6% (weight) and the alcohol with a concentration of 65-75% (weight) are mixed according to a weight ratio of 1:4, and then the glass slide is placed on the obtained Soak in hydrochloric acid alcohol mixture for 2 hours, rinse with distilled water after taking out, and dry naturally;

[0040] 3) Shake the raw milk ...

Embodiment 3

[0045] Embodiment 3 carries out computer identification and counting to the sample obtained in embodiment 1

[0046] With the sample obtained according to the pretreatment in Example 1, the computer identification and counting of somatic cells are carried out by using the existing computer identification and counting methods: first, through a biological microscope (44X type 3 biological microscope, Shanghai Optical Instrument Factory) and an image device (DH-HV2001UC digital video camera, Daheng Optoelectronics Technology Co., Ltd.) acquires digital images of somatic cells in raw milk, and then collects and stores the digital images by computer, and then uses intelligent image recognition software (WSW intelligent image processing software, V1. 0, developed by the Institute of Artificial Intelligence of Huazhong University of Science and Technology) to extract the features of the somatic cell image, and compare and analyze and make statistics with the standard feature image in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preprocessing raw milk, in particular to the method for preprocessing the raw milk which is used for computer identification and counting of somatic cells in the raw milk. The method comprises the following steps of: 1) diluting the raw milk with water according to the weight ratio of a diluted sample to undiluted raw milk of (1-3):1; 2) hydrolyzing the diluted sample by using subtilisin solution at the concentration of between 8 to 9.5 weight percent at the temperature of between 20 and 30 DEG C for 4 to 6 minutes, wherein the subtilisin solution is added according to the volume ratio of the diluted sample to the subtilisin solution of (2.9-3.1):(0.9-1.1); and 3) dyeing the hydrolyzed sample by using acridine orange solution at the concentration of between 5 and 6 weight percent for 7 to 8 minutes, wherein the acridine orange solution is added according to the volume ratio of the hydrolyzed sample to the acridine orange solution of (98-102):1.

Description

technical field [0001] The invention relates to a raw material milk pretreatment method, in particular to a raw material milk pretreatment method for computer identification and counting of somatic cells in raw milk. Background technique [0002] "Raw milk" is also called "raw milk", which usually refers to raw liquid milk (including cow's milk, goat's milk, etc.) that has not been processed and processed (such as heating and sterilization) squeezed out from the udder of dairy animals. [0003] There are no somatic cells of dairy animals in normal raw milk. When dairy animals suffer from mastitis, there are non-dairy cell bodies mixed in raw milk. The increase of somatic cells reflects the degree of mastitis in dairy animals and affects the quality of raw milk. The increase of somatic cells is likely to lead to the following consequences: the decomposition of milk fat and milk protein, resulting in rancid smell; heat-resistant enzymes destroy protein, resulting in a decreas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/24C12Q1/06
Inventor 赵化平赵秀忠刘刚
Owner CHINESE ACAD OF AGRI MECHANIZATION SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com