Improved DNA ammoniacal silver dyeing method and method for detecting DNA

Abstract

The present invention relates to a method for dyeing DNA on polyacrylamide gel, that is, the ammoniacal silver dyeing method, comprising steps of first washing, infiltration, second washing, color reaction and termination of color reaction, rather than solidification and sensitization. In addition, the invention also relates to a method for detecting DNA on polyacrylamide gel and a kit for the method.

Description

technical field [0001] The invention belongs to the technical field of biological detection. Specifically, the invention relates to a method for staining DNA on a polyacrylamide gel, which improves the comprehensive performance in terms of detection sensitivity and convenient operation. In addition, the present invention also relates to methods for detecting DNA, kits for these methods, and the like. Background of the invention [0002] Currently, separating DNA by electrophoresis on a polyacrylamide gel and developing (visualizing) the DNA bands has become one of the most commonly used methods in biological detection. Among them, fluorescent dyes, visible organic dyes, silver, and the like can be used to visualize DNA strips on polyacrylamide gels. Visible organic dyes, such as methylene blue [1], brilliant cresyl blue [2], crystal violet [3], and Nile blue [4,5], etc., have low sensitivity and time-consuming operation. Fluorescent dyes, such as ethidium bromide (EB), SYB...

AI Technical Summary

Problems solved by technology

One is called silver-ammonia staining, which uses silver-ammonia solution to infiltrate the gel and uses acid solution containing formaldehyde to develop the color, but its sensitivity is low and the operation process is time-consuming [12]; the other uses acid Silver ion solution staining, in which the gel is impregnated in

Images