The invention discloses
a DNA flexagon nano structure-nanogold
biosensor based on adapter modification and a preparing method and application of the
DNA flexagon nano structure-nanogold
biosensor. The
biosensor comprises
DNA flexagon and nanogold, the
DNA flexagon comprises an adapter
nucleotide sequence of a detected object, and the nanogold is nanogold modified by
a DNA fragment complementary with the adapter
nucleotide sequence. The method includes the following beneficial effects that the DNA flexagon has the accurate space
addressability, and the complicated marking step is avoided after the nanogold is added; an adapter can be synthesized in batch with a chemical method, so that the cost is greatly reduced; the shortcoming that the
autoimmunity of micromolecule matter is poor is overcome; the DNA flexagon nano structure-nanogold biosensor has the lower
detection limit, and LOD reaches the nanogram stage; an atomic force
microscope and
electrophoresis are adopted for conducting representation, qualitative and quantitative analysis is carried out by using the characteristic that the nanogold is high in light in an atomic force
microscope height map; and compared with a traditional survey method, the advantages of being easy and convenient, reliable, lower in requirement for experiment conditions and the like are achieved.