A Rapid Identification Method of Gray Feather Hybrid in Chinese Goose Species

Abstract

The invention discloses a method for rapidly identifying hybrids of gray feathers of Chinese goose species in the field of goose species detection. S1: collecting biological samples of gray feather individuals to be detected; S2: extracting genomic DNA from the biological samples of the individuals to be detected; S3: Using the extracted genomic DNA as a template, the designed and synthesized PCR primer combination is used as primers for conventional PCR reaction; S4: Use 1% agarose gel electrophoresis to separate PCR products, use DL2000 as DNAmarker, and Goldview I as DNA staining S5: Observe the amplified product under ultraviolet light or gel imaging system, if there is an amplified band at about 300bp, it is judged that the detected individual is a heterozygous individual, otherwise, if there is no amplified band at about 300bp, Then it is judged that the detected individual is a homozygous gray feather individual. Quickly and accurately identify gray feather heterozygous individuals, so as to eliminate them from the reserved breeding geese. Only one generation of detection is needed to make the breeding group achieve homozygous gray feather phenotype, improve breeding efficiency, and increase the purity of the population. Identify the method provided.

Description

A method for rapid identification of Chinese geese hybrids with gray feathers technical field The present invention relates to the technical field of goose species detection, be specially a kind of method for quickly identifying Chinese goose species ash-feather hybrids. Law. Background technique my country's meat goose output accounts for about 94% of the world's total, and the goose industry has obvious advantages and characteristics. With the rapid development, the purebred breeding and hybrid production of geese are also developing continuously. Except for Xinjiang Yili goose, all Chinese goose species originated from Swan geese. From the perspective of feather color, it is divided into two types: gray-feathered goose species and white-feathered goose species. Compiled by the National Livestock and Poultry Genetic Resources Committee in 2011 In the edition of "Chinese Livestock and Poultry Genetic Resources, Poultry", there are 17 Chinese grey-feathered goose ...

AI Technical Summary

Problems solved by technology

However, gray goose species generally have the disadvantage of low egg production. In breeding goose production, gray feather male geese and white feather female geese with relatively high reproductive rate are often crossed to produce gray feather commercial geese.

Whether the gray goose on the market is a homozygous local breed, or a hybrid offspring of gray goose and white goose, so far, there is no quick and accurate identification method

Images