Method for making DNA logic integrated circuit
A technology of deoxyribonucleic acid and integrated circuits, applied in circuits, semiconductor/solid-state device manufacturing, electrical components, etc., can solve problems that have never been mentioned before
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[0036] Device preparation and integration embodiment description
[0037] (1) DNA sample preparation:
[0038] 1. Single-stranded DNA was purchased from Perkin Elmer Taiwan Branch; pBR322-3A2 (18bp), pBR322-5S2 (18bp).
[0039] 2. Prepare double-stranded DNA by polymerizing chain reaction (PCR); use the commercial kit GeneAmp PCR Reagent Kit and Ber Taq DNA polymerase Kit to synthesize DNA.
[0040] 3. Alcohol precipitation method (using the principle of extremely low solubility of salts formed by DNA in alcohol to separate DNA from other impurities).
[0041] (1) Add 10-20 μL of 3.0 sodium acetate to 1ml of the solution and mix well.
[0042] (2) Add 1mL of 95% alcohol, mix well, and place at -20°C for about 30-40 minutes.
[0043] (3) Centrifuge at 13,000 rpm for 10 minutes at 4°C, and carefully remove the supernatant.
[0044] (4) Add 0.5 mL of ethanol to wash the DNA precipitate, centrifuge at 13,000 rpm at 4°C for 10 minutes, carefully remove the supernatant to obtai...
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