GSTM1 typing test method based on quantitative PCR technique

Abstract

The invention discloses a GSTM1 typing test method based on a quantitative PCR technique. The typing test method adopts GSTM1 gene-specific primers, SEQ1 and SEQ2, to amplify a GSTM1 gene segment, and meanwhile a GSTM1 gene-specific TAQman probe, SEQ3-Fam, is designed in an amplified region which is defined by the GSTM1 gene-specific primers; ALbumin-specific PCR primers, SEQ4 and SEQ5, are adopted to amplify an Albumin gene segment, and meanwhile an ALbumin gene-specific TAQman probe, SEQ6-Vic, is designed in an amplified region which is defined by the ALbumin gene-specific primers. The typing test method can effectively prevent PCR product pollution, avoid contamination by poisonous DNA colouring agents, greatly simplify test and result analyzing processes, and increase test throughput.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a GSTM1 typing detection method based on quantitative PCR technology. Background technique [0002] GSTM1 is an important metabolic enzyme involved in the transformation and metabolism of endogenous and exogenous substances, endowing metabolites with new biological properties. GSTM1 is polymorphic in humans, and GSTM1 deletion is a common genotype. There are differences in the transformation function of the individual geomaterials of different GSTM1 genotypes. GSTM1 genotyping is used in the study of various biological traits. Traditionally, since the GSTM1 deletion polymorphism can be detected by ordinary PCR, conventional gene-specific PCR and agarose electrophoresis detection methods are often used to confirm the GSTM1 genotype. Although it is simple, it is easy to cause laboratory pollution because the electrophoresis operation must be opened to add samples and elec...

AI Technical Summary

Problems solved by technology

Although it is simple, it is easy to cause laboratory pollution because the electrophoresis operation must open the tube to add samples and electrophoresis, and it is difficult to ensure the reliability of the experiment under high throughput

At the same time, the conventional PCR electrophoresis analysis method is also difficult to find the heterozygous genotype, which affects the accurate determination of gene traits analysis.

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