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Microbial metabolite microbial agent for promoting plant growth, and preparation method and application thereof

A technology for promoting plant growth and microbial metabolism, applied in plant growth regulators, plant growth regulators, botanical equipment and methods, etc. Growth, increase plant yield, and promote the effect of growth

Pending Publication Date: 2022-02-11
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are countless types of microorganisms including bacteria and fungi. At the same time, due to the different metabolites produced by different microorganisms, the existing microbial fertilizers have irrational functions and uneven levels of fertilizer efficiency, and can simultaneously promote There are no related reports on plant root growth and microbial fertilizers that induce up-regulation of auxin-related gene expression

Method used

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  • Microbial metabolite microbial agent for promoting plant growth, and preparation method and application thereof
  • Microbial metabolite microbial agent for promoting plant growth, and preparation method and application thereof
  • Microbial metabolite microbial agent for promoting plant growth, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Prepare medium:

[0064] Cha's medium: 30.0g sucrose, NaNO 3 3.0g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 4H 2 O0.01g, K 2 HPO 4 1.0g, 1.0L distilled water, pH 7.0~7.2;

[0065] PDA medium: 200g potatoes, 20g glucose, dilute to 1000mL with deionized water;

[0066] Malt extract medium: malt extract 20.0g, tap water 1.0L, natural pH.

[0067] The above-mentioned culture medium was sterilized at 121° C. for 20 minutes in an autoclave.

[0068] After the culture medium is cooled, scrape the colonies of Penicillium citrinum, Penicillium rubrum, Penicillium thomerum, Penicillium grisea and Penicillium decumbens into a 250mL Erlenmeyer flask filled with 100mL sterile water, at 120r / min Shake for 30 minutes to fully disperse the spore mass under certain conditions, wherein the culture temperature of Penicillium citrinum is 28°C, and the culture temperature of Penicillium rubrum, Penicillium Thomme, Penicillium grisea and Penicillium decumbens is 25°C. Use a pipette gu...

Embodiment 2

[0072] Get Penicillium citrinum metabolite dry powder 40g prepared in embodiment 1, Penicillium rubrum metabolite dry powder 20g and Penicillium recumbent metabolite dry powder 20g, mix (Penicillium citrinum metabolite dry powder, Penicillium rubrum metabolite dry powder and recumbent The mass ratio of the Penicillium metabolite dry powder is 2:1:1), and the bacterial agent of the microbial metabolite is prepared.

Embodiment 3

[0074] Get the Penicillium rubrum metabolite dry powder 20g prepared in Example 1, the Penicillium rubrum metabolite dry powder 60g and the Penicillium grisea Penicillium metabolite dry powder 40g, mix (the Penicillium rubrum metabolite dry powder, the Penicillium rubrum metabolite dry powder The mass ratio of the dry powder of the metabolite of Penicillium grisea and Penicillium grisea is 1:3:2), and the bacterial agent of the microbial metabolite is prepared.

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Abstract

The invention belongs to the technical field of fertilizer preparation, and particularly relates to a microbial metabolite microbial agent for promoting plant growth, and a preparation method and application thereof. By compounding metabolite dry powders of penicillium citrinum, penicillium rubrum and penicillium decumbens, or the penicillium rubrum, penicillium tomummii and penicillium griseofulvum, the growth of plant root systems can be remarkably promoted, and particularly, the root length, the root diameter and the root weight of the root systems of the root vegetables can be increased, so that the effect of increasing the plant yield is achieved. Meanwhile, the microbial metabolite microbial agent disclosed by the invention can also enable the expression of auxin related genes such as auxin transport genes OsPIN1 and OsPIN9, auxin signal genes OsIAA6, OsIAA9 and OsIAA20 and an auxin receptor gene OsTIR1 to be up-regulated, so that the content of endogenous hormones in plants is increased, and the growth of the plants is promoted.

Description

technical field [0001] The invention belongs to the technical field of fertilizer preparation, and in particular relates to a microbial metabolite microbial agent for promoting plant growth, a preparation method and an application thereof. Background technique [0002] Auxin (IAA) is a very important hormone in plants, which can regulate the growth and development of plants. Auxin is widely distributed in plants, but it is mainly concentrated in vigorously growing parts such as coleoptiles and root tips. It has the effect of promoting plant growth, and there is a concentration effect. Low concentration promotes growth, and vice versa, it inhibits growth. The prior art discloses many auxin-related genes and proteins, such as TIR1 / AFBs proteins identified as IAA receptors, Aux / IAA transcriptional repressors of the IAA signal transduction pathway, ARF transcription factors, and IAA export proteins (PINs) and IAA import protein (AUX1 / LAX), etc. [0003] The root is one of the ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/36A01P21/00C12R1/80
CPCC12N1/14A01N63/36Y02A40/10
Inventor 牟宗敏陈穗云赵大克李倩倩
Owner YUNNAN UNIV
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