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Compound microorganism live bacteria preparation for degrading papermaking wastewater COD, preparation method and applications thereof

A technology for compound microorganisms and live bacteria preparations is applied in the field of degrading papermaking wastewater COD compound microorganism live bacteria preparations and its preparation, which can solve the problems of difficult and high-efficiency microorganisms in the activated sludge method, difficult degradation of COD, and high treatment costs, and improve the color of sewage. Degree and water quality, efficient degradation, low cost effect

Inactive Publication Date: 2014-09-17
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the problem that COD is difficult to degrade in the papermaking wastewater treatment technology in the prior art, and it is difficult to adjust the activated sludge method to obtain high-efficiency microorganisms. It is limited to specific strains, or requires special domestication and cultivation of strains, high treatment costs, low efficiency, etc., and provides a method that can effectively, rapidly and efficiently degrade papermaking wastewater COD, improve sewage chroma and Composite microbial live bacteria preparation for water quality and its preparation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Carry out amplified culture respectively:

[0040] Penicillium decumbens CICC40674 and Myrothecium Verrucaria CGMCC3.2190 were activated on a slant, and were respectively connected with liquid medium according to the inoculum size of 5%, and the liquid medium formula was: soybean powder 1.5%, starch 1%, yeast extract 0.2%, glucose 1%, the rest is water, pH 6.5, percentage is weight and volume percentage, fermented at 25°C for 52 hours.

[0041] After the bacillus massiliensis (Bacillus massiliensis) MCCC 1A00013 is activated on the slant, it can be connected to the liquid medium according to the inoculum size of 5%. The liquid medium formula is: glucose 1%, malt powder 0.2%, yeast extract 0.2%, sodium acetate 0.5% %, the rest is water, pH 7.0, percentage is weight volume percentage, cultivated at 30°C for 52 hours.

[0042] Live bacteria count (conventional live bacteria dilution counting method, the same below), detect the number of bacteria, and mix according to ...

Embodiment 2

[0046] (1) Carry out amplified culture respectively:

[0047] Penicillium decumbens CGMCC3.3195, Penicillium decumbens CGMCC3.524 and Myrothecium Verrucaria (Myrothecium Verrucaria) CGMCC3.2190, Myrothecium verrucaria Bacteria (Myrothecium Verrucaria) CCTCCAF93168 was activated on a slant, and received liquid medium according to the inoculum size of 5%. The liquid medium formula was: soybean powder 1.5%, starch 1%, yeast extract 0.2%, glucose 1%, and the rest were water. pH 6.5, percentages are weight volume percentages, fermented at 25°C for 44 hours.

[0048] After the bacillus massiliensis (Bacillus massiliensis) MCCC 1A00013 is activated on the slant, it can be connected to the liquid medium according to the inoculum size of 5%. The liquid medium formula is: glucose 1%, malt powder 0.2%, yeast extract 0.2%, sodium acetate 0.5% %, the rest is water, pH 7.0, the percentage is weight and volume percentage, cultivated at 37°C for 45 hours.

[0049] Viable bacteria count, the...

Embodiment 3

[0053] Penicillium decumbens CICC40674, Penicillium decumbens CICC40361, Penicillium decumbens CGMCC3.3195, Penicillium decumbens CGMCC3.524 and Mycobacterium verrucosus ( Myrothecium Verrucaria) CGMCC3.2190, Myrothecium Verrucaria CGMCC3.781, and Myrothecium Verrucaria CCTCCAF93168 were activated on the inclined plane, and received liquid medium according to the inoculum size of 5%, and the liquid medium formula It is: soybean powder 1.5%, starch 1%, yeast extract 0.2%, glucose 1%, the rest is water, pH 6.5, percentage is weight and volume percentage, fermented at 25°C for 42 hours.

[0054] After the bacillus massiliensis (Bacillus massiliensis) MCCC 1A00013 is activated on the slant, it can be connected to the liquid medium according to the inoculum size of 5%. The liquid medium formula is: glucose 1%, malt powder 0.2%, yeast extract 0.2%, sodium acetate 0.5% %, the rest is water, pH 7.0, percentage is weight volume percentage, cultivated at 37°C for 52 hours.

[0055] Via...

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Abstract

The invention discloses a compound microorganism live bacteria preparation for degrading papermaking wastewater COD, a preparation method and applications thereof. The preparation contains Penicillium decumbens, Myrothecium Verrucaria, Bacillus massiliensis and a solid fermentation medium. The preparation method comprises the following steps: first, Penicillium decumbens and Myrothecium Verrucaria after inclined plane activation are inoculated in a liquid medium respectively, and cultured at the temperature of 25 DEG C -30 DEG C for 44-52 h to obtain a microorganism bacteria liquid; Bacillus massiliensis after inclined plane activation is inoculated in a liquid medium, and cultured at the temperature of 28 DEG C-37 DEG C for 40-52 h to obtain a microorganism bacteria liquid; second, the microorganism bacteria liquids are mixed uniformly, placed in a solid fermentation medium and fermented and cultured at the temperature of 30 DEG C for 40-80 h, and the processes of drying at the temperature of 40 DEG C-60 DEG C, crushing to 15-50 meshes and sieving are performed. The preparation can degrade papermaking wastewater COD effectively, rapidly and efficiently in applications as a COD-degrading enhancer in the papermaking wastewater treatment process. The preparation can improve wastewater chromaticity and water quality.

Description

technical field [0001] The invention belongs to the field of microbial environmental protection, and in particular relates to a preparation for degrading paper-making wastewater COD composite microbial living bacteria, a preparation method and application thereof. Background technique [0002] Papermaking wastewater can be roughly divided into: pulping cooking liquor, washing wastewater, bleaching wastewater and paper machine white water. Alkali pulp cooking waste liquor, also known as "black liquor", is the main source of pollution in pulp mills. The composition of pulping and papermaking wastewater is complex, and its composition not only depends on the method of pulping, but also depends on many factors such as the type of product and the type of raw material. Suspended substances in wastewater mainly come from fibers and fillers lost in various processes of pulping and papermaking; BOD mainly comes from pulping and cooking processes, such as sugars, alcohols, organic ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C12N1/14C12R1/07C12R1/645C12R1/80C02F103/28
Inventor 郑玉果周斌周纪祥胡海峰
Owner SHANGHAI INST OF PHARMA IND CO LTD
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