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Method for increasing enzyme activity of cellulase produced from fungal

A technology of cellulase and cellulose, applied in the biological field, achieves the effect of simple and feasible operation, improved enzyme activity and obvious effect

Inactive Publication Date: 2012-10-31
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Farnesol is an important quorum-sensing signaling molecule in eukaryotic biochemical communication, but there is no report on the use of farnesol to improve cellulase activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Penicillium decumbens is selected as the production strain, and the formula of the fermentation medium used is as follows: 0.6% of microcrystalline cellulose; 3% of bran; 3% of corn stalk. Inorganic salt solution composition: potassium dihydrogen phosphate 3.0g / l, ammonium sulfate 2.0g / l, MgSO 4 ·7H 2 O 0.5g / l, CaCl 2 2H 2 O 15mg / l etc., pH 5.5.

[0017] Take the activated strains on the 3-ring plate, inoculate them in the liquid medium, and cultivate them with shaking at 30° C. for 3 days with the rotating speed of the shaker at 180 rpm, and use them as seeds. The above seeds were inoculated into the fermentation medium with an inoculum size of 10%, shaken at 30° C. for 3 days, and the rotation speed of the shaker was 180 rpm. On the third day of fermentation culture, farnesol was added, and the final concentration of farnesol in the medium was 1 mM. Samples were taken at different fermentation time points to measure cellulase activity. The definition of filter p...

Embodiment 2

[0019] Trichoderma reesei is selected as the production strain, and the formula of the fermentation medium used is as follows: microcrystalline cellulose 0.5%; bran 3.5%; rice straw powder 3%. Inorganic salt solution composition: potassium dihydrogen phosphate 3.0g / l, ammonium sulfate 1.0g / l, MgSO 4 ·7H 2 O 0.5g / l, CaCl 2 2H 2 O 10mg / l, etc., pH5.5.

[0020] Take the activated strains on the 3-ring plate, inoculate them in the liquid medium, and cultivate them with shaking at 30° C. for 4 days with the rotating speed of the shaker at 180 rpm, and use them as seeds. The above seeds were inoculated into the fermentation medium with an inoculum size of 10%, shaken at 30° C. for 4 days, and the rotation speed of the shaker was 180 rpm. On the fourth day of fermentation culture, farnesol was added, and the final concentration of farnesol in the medium was 0.5 mM. Samples were taken at different fermentation time points to measure cellulase activity. Taking the fermentation sa...

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PUM

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Abstract

The invention relates to a new technical method for increasing the enzyme activity of cellulase. In the fermentation and production process of the cellulase produced from fungal, farnesol is added to increase the enzyme activity of cellulase. By adding farnesol, the filter paper enzyme activity of the Trichoderma reesei cellulase-producing is increased by about 30% and the filter paper enzyme activity of Penicillium decumbens cellulase-producing is increased by about 46%. The method in the invention is simple and practical, the effect of increasing the enzyme activity of cellulase is obvious;and the method can be widely used and can be used in various cellulase-producing industries adopting fungal fermentation.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to improving the enzyme activity of cellulase by adding farnesol in the process of fungal cellulase fermentation and production. Background technique: [0002] Cellulase is an important resource, which is widely used in food, brewing, feed, textile and other industrial technology fields, and its role in the industrial technology of cellulose biomass resource is becoming more and more prominent. Under the background of the increasing scarcity of petrochemical energy and environmental pollution, the use of renewable cellulosic biomass to obtain fiber-based products and energy clearly has great potential and broad market prospects. The key step in the process of cellulose biomass resource utilization is the biotransformation process of cellulose (enzymatic hydrolysis of cellulose), so it is very important to increase the enzyme activity of cellulase. The cellulase produced by fungi has a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/80C12R1/66C12R1/685C12R1/885
Inventor 庄国强马安周吕迪郭红光张洪勋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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