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A method of obtaining high purity orange peel in spider fragrance residue

A technology of hesperetin and spider scent, which is applied in the field of obtaining high-purity hesperetin, can solve the problems of low yield, large environmental impact, and high cost, and achieve easy industrial production, improved enzymatic conversion rate, and resource utilization high effect

Active Publication Date: 2016-03-23
SHAANXI DONGKE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the hesperetin content in the plant is very low, the cost of obtaining hesperetin by the direct separation method is high, and the yield is low, which is not suitable for industrialized production; Suitable for industrial production; the microbial transformation method requires harsh production conditions, which makes the cost of obtaining hesperetin high and is not suitable for industrial production; the hydrolysis method is mainly to isolate hesperidin from Rutaceae plants (orange, pomelo, citrus aurantium, etc.) Then carry out acid hydrolysis or first carry out hydrolysis to isolate hesperetin. This method has moderate cost, but has a great impact on the environment and is not suitable for large-scale promotion.

Method used

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  • A method of obtaining high purity orange peel in spider fragrance residue
  • A method of obtaining high purity orange peel in spider fragrance residue
  • A method of obtaining high purity orange peel in spider fragrance residue

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Experimental program
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Effect test

Embodiment 1

[0061] By analyzing the results of the above extraction and enzymatic hydrolysis process, it is determined that the best method for extracting and separating hesperetin from the spider incense residue after extracting total terpenes of the present invention includes the following steps:

[0062] 1) Take 10kg of the spider incense extract residue after extracting the total terpenes, with a hesperidin content of 1%, and extract twice at 90°C with an aqueous solution of calcium hydroxide with a mass concentration of 5%, and the first addition is 80L. 2h, the second addition is 60L, extract for 2h, combine and filter the extracts, adjust the pH value to 8-9 with 5% calcium hydroxide aqueous solution, and set aside;

[0063] 2) Put the pH-adjusted extract on a macroporous resin column, the resin type is AB-8, the pH value is 8-9, the volume of the extract is 120L, and the adsorption flow rate is 1.5BV / h. After the adsorption is saturated, use Elute with deionized water. When the ef...

Embodiment 2

[0077] 1) Take 10kg of the spider incense extract residue after extracting the total terpenes, with a hesperidin content of 1%, and extract twice at 90°C with an aqueous solution of calcium hydroxide with a mass concentration of 5%, and the first addition is 80L. 2h, the second addition is 60L, extract for 2h, combine and filter the extracts, adjust the pH value to 8-9 with 5% calcium hydroxide aqueous solution, and set aside;

[0078] 2) Put the pH-adjusted extract on a macroporous resin column, the resin type is AB-8, the pH value is 8-9, the volume of the extract is 120L, and the adsorption flow rate is 1.5BV / h. After the adsorption is saturated, use Elute with deionized water. When the effluent is clarified, the washed column packing is eluted with sodium hydroxide eluent with a mass concentration of 0.8% to 1%. The amount of eluent is 50L, and the flow rate is 1BV / h. eluent;

[0079] 3) Pour the obtained eluent into the reaction kettle, adjust the pH to 4 with 5% hydroch...

Embodiment 3

[0083] 1) Take 10kg of the spider incense extract residue after extracting the total terpenes, with a hesperidin content of 1%, and extract twice at 90°C with an aqueous solution of calcium hydroxide with a mass concentration of 5%, and the first addition is 80L. 2h, the second addition is 60L, extract for 2h, combine and filter the extracts, adjust the pH value to 8-9 with 5% calcium hydroxide aqueous solution, and set aside;

[0084] 2) Put the pH-adjusted extract on a macroporous resin column, the resin type is AB-8, the pH value is 8-9, the volume of the extract is 120L, and the adsorption flow rate is 1.5BV / h. After the adsorption is saturated, use Elute with deionized water. When the effluent is clarified, the washed column packing is eluted with sodium hydroxide eluent with a mass concentration of 0.8% to 1%. The amount of eluent is 50L, and the flow rate is 1BV / h. eluent;

[0085] 3) Pour the obtained eluent into the reaction kettle, adjust the pH to 6 with 5% hydroch...

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Abstract

A disclosed method for obtaining high-purity hesperetin from valeriana jatamansi residue comprises: heating valeriana jatamansi residue from which total terpenoid is extracted, leaching for 2 times, adjusting pH of the leaching solution, utilizing macroporous adsorption resin column for gathering hesperetin, firstly eluting with deionzed water, when the eluate is cleared, then eluting with a sodium hydroxide solution with a mass concentration of about 1%, gathering the eluate, adjusting the pH value of the eluate, and performing enzymatic hydrolysis, centrifugation filtering and recrystallization to obtain high-purity hesperetin which is light yellow acicular crystal. The method is simple in operation, low in production cost and high in source utilization rate, and is easy for industrialized production.

Description

technical field [0001] The invention relates to a method for extracting and separating effective components of traditional Chinese medicines, in particular to a method for obtaining high-purity hesperetin from spider incense dregs. Background technique [0002] Spider incense is the dried rhizome and root of Valeriana plant spider incense (Valerianajatamansi Jones). Distributed in my country's Sichuan, Guizhou, Yunnan, Hunan, Hubei, Shaanxi and other places. It has the effects of dispelling wind-heat, relieving sore throat, clearing rash, improving eyesight and removing nebula, and relieving spasm. For the treatment of wind-heat and cold, sore throat and hoarseness, impermeable measles, rubella and itching, convulsions and convulsions, and tetanus. Pharmacological studies have shown that spider incense can act on the central nervous system, with sedative, hypnotic, anticonvulsant and anti-anxiety effects; it can also treat viral diarrhea, seasonal diarrhea and irritable bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
Inventor 申旭霁杜杉孙颖夏传涛
Owner SHAANXI DONGKE PHARMA
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