Comprehensive utilization method of lignocellulose biomass

A lignocellulose and biomass technology, which is applied in the field of comprehensive utilization of cellulose, hemicellulose and lignin in lignocellulosic biomass, can solve problems such as low conversion rate of cellulose enzymatic hydrolysis, improve extraction rate, simplify Production process, the effect of reducing dosage

Active Publication Date: 2013-04-17
JINAN SHENGQUAN GROUP SHARE HLDG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is to overcome the problem of low cellulose enzymatic conversion rate in the prior art when comprehensively utilizing hemicellulose, lignin and cellulose, thereby proposing a cellulose enzymatic conversion rate high Comprehensive utilization method of lignocellulosic biomass

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  • Comprehensive utilization method of lignocellulose biomass

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Experimental program
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Effect test

Embodiment 1

[0048] (1) acid hydrolysis

[0049] 10.6kg corn cobs (mass composition: moisture 6.12%, cellulose 35.19%, hemicellulose 32.1%, lignin 23.7%, other 2.95%, the same below) are broken, washed with water to remove dust, and then processed with 80kg phosphoric acid solution Hydrolysis, the mass concentration of phosphoric acid solution is 15%, the temperature of acid hydrolysis is 140 ℃, the time is 0.5 hour, the acid hydrolysis residue and the pentose sugar solution obtained after hydrolysis are separated, wash described acid hydrolysis residue with 10kg water, cleaning solution Merge with described pentose solution, finally obtain 17.9kg acid hydrolysis residue (moisture content is about 65%, the absolute dry content of hemicellulose is 13.03%, the absolute dry content of lignin is 32.67%, the absolute dry content of cellulose 49.27%) and 82.07kg pentose solution, the concentration of pentose solution is 3.16%, and the extraction rate of hemicellulose is 76%.

[0050] The calcul...

Embodiment 2

[0062] (1) acid hydrolysis

[0063] Break 10.6kg of corncobs, wash with water to remove dust, and then hydrolyze with 80kg of phosphoric acid solution, the mass concentration of phosphoric acid solution is 20%, the temperature of acid hydrolysis is 100°C, and the time is 2 hours. After the hydrolysis is completed, the separated acid hydrolysis Residue and pentose solution, wash described acid hydrolysis residue with 10kg water, and cleaning solution merges with described pentose solution, finally obtain 16.42kg acid hydrolysis residue (moisture content is about 65%, and the absolute dry content of hemicellulose is 11.86 %, the dry content of lignin is 32.72%, the dry content of cellulose is 49.95%) and 83.56kg pentose solution, the concentration of pentose solution is 3.26%, and the extraction rate of hemicellulose is 80%.

[0064] (2) Enzymatic hydrolysis of cellulose

[0065] Take the acid hydrolysis residue described in step (1) as the fiber substrate, and carry out cellul...

Embodiment 3

[0069] (1) acid hydrolysis

[0070] Break 10.6kg of corncobs, wash with water to remove dust, and then use 80kg of phosphoric acid solution for hydrolysis, the mass concentration of phosphoric acid solution is 5%, the temperature of acid hydrolysis is 150 ° C, and the time is 3 hours. Residue and pentose solution, clean described acid hydrolysis residue with 10kg water, and cleaning solution merges with described pentose solution, finally obtains 18.01kg acid hydrolysis residue (water content is about 65%, and the dry content of hemicellulose is 13.52 %, the absolute dry content of lignin is 32.49%, the absolute dry content of cellulose is 49%) and 81.98kg pentose sugar solution, the concentration of pentose sugar solution is 3.12%, and the extraction rate of hemicellulose is 75%.

[0071] (2) Enzymatic hydrolysis of cellulose

[0072] Take the acid hydrolysis residue described in step (1) as the fiber substrate, and carry out cellulose enzymolysis, the condition of the enzym...

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Abstract

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; (b) performing enzymatic hydrolysis of the acid hydrolysis residues in step (a) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2011195; (c) processing the enzymatic hydrolysis residues produced in step (b) by using an alkaline solution so as to extract lignin from the enzymatic hydrolysis residues. The method realizes the maximum resource utilization of lignocellulose biomass.

Description

technical field [0001] The invention relates to a method for comprehensively utilizing lignocellulosic biomass, in particular to a method for comprehensively utilizing cellulose, hemicellulose and lignin in lignocellulosic biomass. Background technique [0002] With the depletion of fossil fuel resources and the increasing environmental pollution, the use of renewable energy as a substitute for petrochemical products has become increasingly important. Fuel ethanol is the main form of biomass liquid energy and the most likely substitute for fossil fuels. At present, the world's ethanol production mainly uses starches (corn, cassava, etc.) and sugars (sugar cane, sugar beets, etc.) as raw materials for fermentation. The technology of producing ethanol by microbial fermentation is mature, but the high cost of raw materials restricts the industrial application of grain fermentation to produce ethanol. At the same time, there are disadvantages such as competition with people for...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02C08H7/00C13K13/00C12R1/80
Inventor 唐一林江成真高绍丰张恩选马军强栗昭争赵兴国郭雨霖
Owner JINAN SHENGQUAN GROUP SHARE HLDG
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