Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting and separating kaempferol from Ligustrum sinense branches and leaves

A technology of kaempferol and branches, which is applied in the field of extraction and separation of kaempferol, can solve the problems of complex process, low yield, and low kaempferol content, and achieve the effects of simple operation, low production cost, and improved utilization rate

Active Publication Date: 2013-01-02
SHAANXI DONGKE PHARMA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The method for preparing kaempferol at present mostly is to directly separate kaempferol from the original plant, and its kaempferol content is low, and its process is relatively complicated, and the yield is low
And the method that utilizes enzymatic hydrolysis to extract and separate kaempferol from plants has not yet been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention will be further described in detail below in conjunction with the examples given by the inventor.

[0014] The method for extracting kaempferol from the glabra twigs and leaves of the present invention comprises the following steps:

[0015] Firstly, take 10kg of the powder of the branches and leaves of A. glabrata, the content of kaempferoside is 0.9%, extract twice with calcium hydroxide water with a mass concentration of 5%, the first addition is 80L, and extract under the condition of 80℃~90℃ 2h, the second addition is 50L, extract at 80°C-90°C for 2h, combine the two extracts, adjust the pH of the extract to 8-9, and set aside;

[0016] Put the extract after adjusting the pH value on the macroporous resin column, the resin model is AB-8, the pH value is 8-9, the volume of the sample loading extract is 60L, and the adsorption flow rate is 1.8 times / h; after the adsorption is saturated, use a deionized Rinse the column with water washing liquid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting and separating kaempferol from Ligustrum sinense branches and leaves, which comprises the following steps: carrying out thermal-soak extraction on Ligustrum sinense twice, regulating the pH value, enriching with macroporous adsorbent resin, and flushing the columns with deionized water flushing liquid; after the effluent liquid becomes clear, eluting the flushed column filler with 0.8-1 wt% sodium hydroxide eluting liquid, and collecting the eluate; and regulating the pH value of the eluate, carrying out enzymolysis, crystallizing, filtering, and recrystallizing multiple times, thereby obtaining the yellow acicular crystal kaempferol refined product. The method has the advantages of low production cost and wide industrialization prospects, is simple to operate and can easily implement industrial production.

Description

technical field [0001] The invention relates to an extraction and separation method, in particular to a method for extracting and separating kaempferol from the branches and leaves of A. glabrata. Background technique [0002] wax tree glabra ( L. sinense Lour var ni ti dum Ruhd ) is the genus Oleaceae Ligustrum Ligustrum Linn plants, evergreen shrubs. The chemical composition research shows that: it mainly contains alkanes, higher fatty alcohols, steroids, triterpenoids, and flavonoids, among which kaempferol is relatively high in content. [0003] Kaempferol ( Kaempferol ), aliases: kaempferol-3 kaempferol, kaempferol flavonol, tetrahydroxyflavone, thymosin III; kaempferol belongs to flavonols (molecular formula C 15 h 10 o 6 Molecular weight 286.23), yellow needle crystal, melting point 276°C-278°C Kaempferol is slightly soluble in water, soluble in hot ethanol, ether and alkali; pharmacological effects: antibacterial, against Staphylococcus aureus and Pseudomon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
Inventor 马毅刚
Owner SHAANXI DONGKE PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com