Method for extracting and separating kaempferol from Ligustrum sinense branches and leaves

A technology of kaempferol and branches, which is applied in the field of extraction and separation of kaempferol, can solve the problems of complex process, low kaempferol content, and low yield, and achieve the effects of simple operation, improved utilization rate, and low production cost

Active Publication Date: 2011-11-23
SHAANXI DONGKE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The method for preparing kaempferol at present mostly is to directly separate kaempferol from the original plant, and its kaempferol content is low, and its process is relatively complicated, and the yield is low
And the method that utilizes enzymatic hydrolysis to extract and separate kaempferol from plants has not yet been reported

Method used

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Embodiment Construction

[0013] The present invention will be further described in detail below in conjunction with the examples given by the inventor.

[0014] The method for extracting kaempferol from the glabra twigs and leaves of the present invention comprises the following steps:

[0015] Firstly, take 10kg of the powder of the branches and leaves of A. glabrata, the content of kaempferol is 0.9%, extract twice with calcium hydroxide water with a mass concentration of 5%, the first addition is 80L, and extract at 80°C-90°C 2h, the second addition is 50L, extract at 80°C-90°C for 2h, combine the two extracts, adjust the pH of the extract to 8-9, and set aside;

[0016] Put the extract after adjusting the pH value on the macroporous resin column, the resin model is AB-8, the pH value is 8-9, the volume of the sample loading extract is 60L, and the adsorption flow rate is 1.8 times / h; after the adsorption is saturated, use a deionized Rinse the column with water washing liquid, and when the efflue...

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Abstract

The invention discloses a method for extracting and separating kaempferol from Ligustrum sinense branches and leaves, which comprises the following steps: carrying out thermal-soak extraction on Ligustrum sinense twice, regulating the pH value, enriching with macroporous adsorbent resin, and flushing the columns with deionized water flushing liquid; after the effluent liquid becomes clear, eluting the flushed column filler with 0.8-1 wt% sodium hydroxide eluting liquid, and collecting the eluate; and regulating the pH value of the eluate, carrying out enzymolysis, crystallizing, filtering, and recrystallizing multiple times, thereby obtaining the yellow acicular crystal kaempferol refined product. The method has the advantages of low production cost and wide industrialization prospects, is simple to operate and can easily implement industrial production.

Description

technical field [0001] The invention relates to an extraction and separation method, in particular to a method for extracting and separating kaempferol from the branches and leaves of A. glabrata. Background technique [0002] wax tree glabra ( L. sinense Lour var ni ti dum Ruhd ) is the genus Oleaceae Ligustrum Ligustrum Linn plants, evergreen shrubs. The chemical composition research shows that: it mainly contains alkanes, higher fatty alcohols, steroids, triterpenoids, and flavonoids, among which kaempferol is relatively high in content. [0003] Kaempferol ( Kaempferol ), aliases: kaempferol-3 kaempferol, kaempferol flavonol, tetrahydroxyflavone, thymosin III; kaempferol belongs to flavonols (molecular formula C 15 h 10 o 6 Molecular weight 286.23), yellow needle crystal, melting point 276°C-278°C Kaempferol is slightly soluble in water, soluble in hot ethanol, ether and alkali; pharmacological effects: antibacterial, against Staphylococcus aureus and Pseudomon...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 马毅刚
Owner SHAANXI DONGKE PHARMA
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