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Cotton stalk hydrolysate and preparation method thereof

A technology for hydrolyzed liquid and cotton stalk, which is applied in the field of cotton stalk hydrolyzed liquid and its preparation, can solve the problems of low conversion rate of alkali-treated cotton stalk, unfavorable for industrial enlargement and high concentration of sugar liquid and salt, achieves good industrial application prospect, and is easy to use. Industrial application, the effect of high glucose content

Active Publication Date: 2013-06-12
新疆梅花氨基酸有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkali treatment of cotton stalks has a low conversion rate, and microwave treatment is not conducive to industrial scale-up
The conversion rate of acid treatment is high, but the amount of reagents is large, the cost is high, the sulfur dioxide produced in the operation pollutes the environment, and the salt concentration in the obtained sugar solution is high, which is not conducive to fermentation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 The preparation of cotton stalk hydrolyzate

[0030] Take 10Kg of cotton stalks, pulverize them with a pulverizer to a particle size of 0.5-1 cm for later use. Weigh 400g of cotton stalk particles, add dilute sulfuric acid with a concentration of 1.2% according to the solid-to-liquid ratio of 1:6, put it in a water bath at 60°C, take it out after soaking for 50 minutes, squeeze it dry until the solid content is 30%, and put it in an air explosion In the tank, gas-explode at 195°C for 10 minutes, then use circulating water to wash and gas-explode the material to a pH of 5.0, add cellulase according to the cellulose content, the amount of enzyme added is 5.5% by mass of the cellulose, add 50% to the enzymolysis tank The enzymatic hydrolysis was carried out at ℃ for 72 hours. After the enzymatic hydrolysis, the hydrolyzate was obtained by suction filtration. The glucose concentration could reach 40g / L, and the enzymatic hydrolysis conversion rate of cotton sta...

Embodiment 2

[0031] Embodiment 2 Preparation of cotton stalk hydrolyzate

[0032] Take 10Kg of cotton stalks, pulverize them with a pulverizer to a particle size of 0.5-1 cm for later use. Weigh 400g of cotton stalk particles, add dilute sulfuric acid with a concentration of 2.8% according to the solid-to-liquid ratio of 1:8, put it in a water bath at 80°C, take it out after soaking for 30 minutes, squeeze it dry until the solid content is 40%, and put it in an air explosion In the tank, gas-explode for 13 minutes at 204°C, then use circulating water to wash and gas-explode the material to a pH of 6.0, add cellulase according to the cellulose content, the amount of enzyme added is 4.0% by mass of the cellulose, add 50% to the enzymolysis tank Enzymolysis at ℃ for 120 hours, after the end of the enzymolysis, the hydrolyzate was obtained by suction filtration, the glucose concentration could reach 50g / L, and the enzymatic hydrolysis conversion rate of cotton stalks was 80%.

Embodiment 3

[0033] Embodiment 3 Preparation of cotton stalk hydrolyzate

[0034] Take 10Kg of cotton stalks, pulverize them with a pulverizer to a particle size of 0.5-1 cm for later use. Weigh 400g of cotton stalk particles, add 4% sodium hydroxide according to the solid-to-liquid ratio of 1:10, put it in a water bath at 90°C, take it out after soaking for 60 minutes, squeeze it dry until the solid content is 40%, put it in air In the explosion tank, gas explosion at 204°C for 7 minutes, then use circulating water to wash the gas explosion material to a pH of 7.0, add cellulase according to the cellulose content, and the enzyme addition accounts for 6.2% of the mass percentage of the cellulose, and add it to the enzymolysis tank Enzymolysis was performed at 50°C for 120 hours. After the end of the enzymolysis, the hydrolyzate was obtained by suction filtration. The glucose concentration could reach 45g / L, and the enzymatic hydrolysis conversion rate of cotton stalks was 75%.

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Abstract

The invention relates to the biotechnological field, especially to a cotton stalk hydrolysate and a preparation method thereof. In the cotton stalk hydrolysate obtained by the method, glucose concentration may be above 40 g / L and the enzyme hydrolysis conversion rate of cotton stalks may be above 70%; therefore, the waste of agricultural wasted resources is effectively solved and a new way is opened for recycling of cotton stalks; and the cotton stalk hydrolysate has an excellent industrial application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cotton stalk hydrolyzate and a preparation method thereof. Background technique [0002] my country is the world's largest cotton demander and importer, and Xinjiang is the main cotton-producing region in my country. In 2005, the cotton planting area in Xinjiang alone reached 17.21 million mu, the cotton output was 1.8 million tons, and the cotton straw output was about 6.5 million tons. In 2011, my country's cotton output was about 6.6 million tons, and about 19 million tons of cotton straw were produced accordingly. Cotton stalk is one of the main by-products of cotton production, and its main components include cellulose, hemicellulose, lignin, protein, pectin, etc. A large amount of cotton stalks are discarded, resulting in a great waste of resources. With the promulgation and implementation of my country's Renewable Energy Law, the utilization of biomass energy will definite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 李必金于宏艳叶华龚华胡征宇
Owner 新疆梅花氨基酸有限责任公司
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