Process for enzymatic hydrolysis of lignocellulosic materila and fermentation of sugars

a technology of lignocellulosic material and enzymatic hydrolysis, which is applied in the direction of enzymology, biofuels, transferases, etc., can solve the problems of inability to optimize the inability to disclose the optimization process of hydrolysis and fermentation, and inability to convert reducing sugar in general slow and incomplete manner. , to achieve the effect of reducing the dosage of enzymes, and reducing the risk of contamination

Inactive Publication Date: 2012-07-19
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0012]An object of the invention is therefore to provide a process in which the hydrolysis step and fermentation step are conducted at optimal temperature conditions. Another object of the invention is to provide a process involving hydrolysis and fermentation having a reduced process time. Further object of the invention is to provide a process, wherein the dosage of enzyme may be reduced and at the same time output of useful fermentation product is maintained at the same level. Another object is to provide a process wherein risk of contamination by contaminating microorganisms, is reduced. Another object is to provide a process wherein the dry-matter content is increased. Another object is to provide a process involving hydrolysis and fermentation, wherein the process conditions of the fermentation are optimized. One or more of these objects are attained according to the invention. The present invention provides a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps:
[0019]Surprisingly, according to the invention, by the provision of an enzyme composition that is stable and has a temperature optimum of 55 degrees C. or more for the enzymatic hydrolysis step c), it is possible to attain many process advantages, including optimal temperature conditions, reduced process time, reduced dosage of enzyme, reduced risk of contamination, higher dry-matter concentrations, re-use of enzymes and other process optimizations, resulting in reduced costs.

Problems solved by technology

Since the (hemi)-cellulose is crystalline and entrapped in a network of lignin, the conversion into reducing sugars is in general slow and incomplete.
In general, costs for enzymes production, feedstock to ethanol yields and investments are major cost factors in the overall production costs [2].
However, no disclosure is given how to optimize the process of hydrolysis and fermentation.

Method used

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  • Process for enzymatic hydrolysis of lignocellulosic materila and fermentation of sugars
  • Process for enzymatic hydrolysis of lignocellulosic materila and fermentation of sugars
  • Process for enzymatic hydrolysis of lignocellulosic materila and fermentation of sugars

Examples

Experimental program
Comparison scheme
Effect test

example 1

Reduction of Process Time by Using Enzyme Composition that Retains Activity for More than 30 Hours at Elevated Temperatures

[0173]A comparison is made between a process in which hydrolysis is extended to 72 hours and a process in which the time for hydrolysis is limited to 20 hours to demonstrate the benefit of using the retained activity of the enzyme composition in an extended hydrolysis.

[0174]Materials and Methods

[0175]The method of hydrolysis and fermentation is described in patent application WO2010018105 (PCT / EP2009 / 060098). Pre-treated wheat straw was used as feedstock and washed in tap water of 70 degrees C. until pH was higher than 3, preferably between 6.0 and 6.5. A slurry of 10% w / w of the washed pre-treated feedstock was prepared and pre-incubated at 56 degree C. Enzyme composition was added at a concentration of 0.20 g enzyme composition per g feedstock dry matter. The mixture was divided in two equal portions A and B and incubated at 56 degree C. in an incubator while ...

example 2

Reduction of Enzyme Dosage by Increasing the Process Time with Enzyme Composition that Retain Active for More than 30 Hours at Elevated Temperatures

[0183]In another experiment, the property of retaining activity of the enzyme composition was used to compensate low enzyme dosage by extended hydrolysis.

Materials and Methods

[0184]The method of hydrolysis and fermentation as referred to in Example 1 was used.

[0185]A slurry of 10% w / w of the washed pre-treated feedstock was prepared and pre-incubated at 56 degree C. The slurry was divided in to two equal portions A and B. Enzyme composition was added at a concentration of 0.20 g enzyme composition per g feedstock dry matter to portion A and at a concentration of 0.09 g enzyme composition per g feedstock dry matter to portion B. The portions A and B were incubated at 56 degree C. in a incubator while smoothly shaken for 220 hours. During this hydrolysis, samples were taken daily to determine the amount of glucose released from the cellulo...

example 3

Use of Enzyme Composition with Retaining Activity Allows re-use of Activity After Hydrolysis

[0190]An experiment is performed to demonstrate the use of the liquid fraction of the feedstock-enzyme slurry after hydrolysis, as a source of activity for the hydrolysis in a next process cycle.

[0191]Materials and Methods

[0192]The method of hydrolysis as referred to in Example 1 is used.

A slurry of 10% w / w of the pre-treated feedstock is prepared and pre-incubated at 60 degree C. Enzyme composition is added at a concentration of 0.20 g enzyme composition per g feedstock dry matter and incubated at 56 degree C. in a incubator while smoothly shaken for 120 hours. The slurry is than taken from the incubator and centrifuged at 4500 g. the pellet is washed once, centrifuged and the washing supernatant is combined with the supernatant of the first solid-liquid separation. The combined supernatant is filtrated, using a Z200 filter (a about 0.2 um thick paperfilter by Pall) and concentrated using a ...

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Abstract

The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps:a) optionally pre-treatmentb) optionally washing;c) enzymatic hydrolysis;d) fermentation; ande) optionally recovery of a fermentation product;wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.

Description

FIELD OF THE INVENTION[0001]The invention relates to a process for the enzymatic hydrolysis of lignocellulosic material and fermentation of sugars.BACKGROUND OF THE INVENTION[0002]Ligno-cellulosic plant material, herein also called feedstock, is a renewable source of energy in the form of sugars that can be converted into valuable products e.g. bio-fuel, such as bio-ethanol. During this process, (ligno or hemi)-cellulose present in the feedstock, such as wheat straw, corn stover, rice hulls, etc., is converted into reducing sugars by (hemi)-cellulolytic enzymes, which then are converted into valuable products such as ethanol by microorganisms like yeast, bacteria and fungi.[0003]Since the (hemi)-cellulose is crystalline and entrapped in a network of lignin, the conversion into reducing sugars is in general slow and incomplete. Typically, enzymatic hydrolysis of untreated feedstock yields sugars <20% of theoretical quantity. By applying a chemical and thermo-physical pre-treatment...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12P35/00C12P7/16C12P7/56C12P7/42C12P7/40C12P7/18C12P7/20C12P7/48C12P7/46C12P13/08C12P13/12C12P13/22C12P13/20C12N9/50C12N9/42C12N9/28C12N9/24C12N9/38C12N9/20C12N9/02C12N9/10C12P13/04C12P1/00C12P5/02C12P17/10C12P7/14
CPCC12P7/10Y02E50/16C12P19/02C12P19/14Y02E50/10C12R2001/645C12R2001/865
Inventor SMITS, JOHANNES PETRUSGIERVELD, ELISABETH MARIAVAN DER HOR, FOP CORNELIS
Owner DSM IP ASSETS BV
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