Method for efficiently saccharifying xylose residue or furfural residue

A technology of furfural residue sugar and xylose residue, which is applied in the field of high-efficiency saccharification of xylose residue or furfural residue, which can solve the problems of affecting the use effect of cellulase, difficulty in meeting the fermentation process, and substrate concentration restrictions, so as to promote industrial recovery and reuse, beneficial to environmental protection, and the effect of reducing the amount of cellulase

Active Publication Date: 2010-01-06
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the increasing use of biomass resources in human society, the discharge of biomass residues generated during the processing of lignocellulosic raw materials such as xylose residues and furfural residues is also increasing, such as random accumulation or digging pits to dump waste , not only will cause secondary dust and environmental pollution, but also cause a great waste of resources
Biomass waste residues produced during the processing of lignocellulosic raw materials such as xylose residues and furfural residues contain a lot of cellulose, which can be used for fermentation to produce sugars. Hydrolysis sugar production, because the waste residue contains about 26% lignin and about 8% hemicellulose, the substrate concentration is limited during enzymatic hydrolysis, the obtained sugar solution has a low concentration and a high miscellaneous sugar content, and the total sugar concentration is about About 8%, the purity of glucose is only about 77%, the sugar solution is difficult to meet the requirements of other fermentation processes; at the same time, due to the existence of lignin, the use effect of cellulase is affected, resulting in an increase in the amount of enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Add xylose slag (solid residue after dilute acid hydrolysis of corncob) with a water content of 70% into the steam explosion machine, open the steam valve and quickly heat up to 200°C, the heating time is 1 minute, and after 15 minutes of heat preservation at 200°C, Close the steam valve, open the steam explosion quick-opening valve of the steam explosion machine to release the material instantly. Collect the material after steam explosion treatment through centrifugation, the gained solid phase adds the water of same weight and adjusts pH to 4.8 with acid, regulates temperature to 50 ℃, then adds cellulase, and enzyme amount is 30FPIU / gram substrate (with dry weight), the stirring speed was 100rpm / min, and the enzymolysis time was controlled at 24 hours. Samples were taken every 12 hours, the total sugar concentration in the enzymolysis solution was measured by DNS method, and the glucose and xylose concentrations in the enzymolysis solution were measured by high perfo...

Embodiment 2

[0022] Add xylose slag with a water content of 60% (the solid residue after dilute acid hydrolysis of corncobs) into the steam explosion machine, open the steam valve and quickly raise the temperature to 190°C, the heating time is 2 minutes, and after 10 minutes of heat preservation, close the steam valve , Open the steam explosion quick-opening valve of the steam explosion machine to release the material instantly. The material after steam explosion treatment is collected and centrifuged, the obtained solid phase is added to the container, and the same weight of water is added at the same time, the pH is adjusted to 4.6 with acid, the temperature is adjusted to 46 °C, and then cellulase is added. The amount of enzyme used is 20 FPIU / Gram substrate (in dry weight), the stirring speed is 100rpm / min, and the enzymolysis time is controlled at 72 hours. Samples were taken every 12 hours, the total sugar concentration in the enzymolysis solution was measured by DNS method, and the...

Embodiment 3

[0024] Add xylose slag with a water content of 80% into the steam explosion machine, open the steam valve and quickly raise the temperature to 220°C, the heating time is 2 minutes, after 2 minutes of heat preservation, close the steam valve, open the steam explosion quick opening valve of the steam explosion machine The material is released instantly. The material after steam explosion treatment is collected and centrifuged, the obtained solid phase is added to the container, and the same weight of water is added at the same time, the pH is adjusted to 5.0 with acid, the temperature is adjusted to 48 °C, and then cellulase is added. The amount of enzyme used is 25 FPIU / Gram substrate (in dry weight), the stirring speed is 100rpm / min, and the enzymolysis time is controlled at 64 hours. Samples were taken every 12 hours, the total sugar concentration in the enzymolysis solution was measured by DNS method, and the glucose and xylose concentrations in the enzymolysis solution wer...

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PUM

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Abstract

The invention relates to a method for efficiently saccharifying biomass wastes such as xylose residue or furfural residue, comprising the following steps: performing steam exploding processing on biomass wastes such as xylose residue or furfural residue, then collecting materials after steam exploding, carrying out solid-liquid separation, and performing enzymolysis and saccharification on a solid phase obtained from separation. In the method of the invention, the crystallizing structure of cellulose is destroyed by steam exploding processing, the specific surface area of cellulose is increased, and the content of lignin is reduced, thereby reducing the use amount of cellulase during saccharification of lignocellulose materials, improving the enzymolysis efficiency, increasing sugar concentration in the enzymolysis solution, effectively reducing the cost for enzymolysis and sugar concentration utilizing lignocellulose materials, promoting industrialized recover and reuse to the waste, and being favorable for protecting the environment and saving resources.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for efficiently saccharifying xylose residue or furfural residue. Background technique [0002] Xylose mainly exists in plants in the form of polysaccharides, especially in agricultural product wastes such as corn cobs, straws, and cotton boll husks. Since xylose is not digested and absorbed after being ingested into the human body, it has no energy value and can satisfy the needs of dessert lovers to the greatest extent. In addition, it can also improve the microbial environment of the human body, activate bifidobacteria in the human intestinal tract and promote their growth. Therefore, in recent years, it has been widely used in food and beverages as a non-caloric sweetener, which is in line with the current social trend and suitable for obese and diabetic patients. However, when using biomass raw materials to produce products such as xylose a...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
Inventor 李荣杰薛培俭黄之文尚海涛
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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