Method for producing glucose and cellobiose using cellulose complex enzyme

Abstract

A method for producing glucose and cellobiose using cellulose complex enzyme is the technology using the cellulose complex enzyme as a catalyst and realizing the high saccharification rate conversion of cellulose by adding cellulose enzyme activator at the optimized stirring speed. Glucose concentration can reach 150g / L after using cellulose enzymatic hydrolysis, and the method is a cellulose saccharification production technology with economic feasibility. With the technology used, low cost, low usage of enzyme and high saccharification rate can be realized, and the productions of cellulose alcohol and biochemical products (lactic acid, succinic acid and the like) are satisfied. In the whole technology, no beta-glucosaccharase is added additionally, no sugar concentrating device is added, and the technology is simple, the equipment cost is low and the industrialized prospect is good.

Description

technical field [0001] The invention relates to a high-efficiency production method of cellulose saccharification process, in particular to a method for saccharifying cellulose by using a compound cellulosic enzyme system and a chemical activator. Background technique [0002] With the increasing depletion of petroleum resources, the increasing shortage of food, and the huge demand for liquid fuels such as ethanol and butanol and other biomass chemical products (lactic acid, succinic acid, etc.), the use of lignocellulose as raw material The research and development of producing fermentable sugar and then biotransforming biochemical products has become a hot spot in the scientific and industrial circles of China, the United States and other world energy-consuming countries. [0003] my country is a large agricultural country, and about 700 million tons of agricultural waste is formed every year, including 110 million tons of wheat straw, 180 million tons of rice straw, 13 mi...

AI Technical Summary

Problems solved by technology

The second is that under the condition of high substrate content of 15% (w / w) or more, a large amount of cellulose is not converted, the enzymolysis effect is significantly reduced, and the conversion rate is approximately reduced to a low concentration (5% w / w) Half of the enzymatic efficiency, usually only 40-50%

[0005] At present, domestic patents have reported three processes related to cellulosic ethanol, but the economic feasibility is not strong

Chinese patent CN101130792A discloses a method for preparing fuel ethanol by enzymolysis of stalks. The fermentation time is 10-15 days, and the enzymolysis efficiency is very low, and there is no economic feasibility for industrialization

Chinese patent CN101270372A discloses a method for concentrating sugar liquid in cellulose ethanol fermentation liquid, the purpose is also to solve the problem that the sugar concentration after enzymatic hydrolysis is too low, which shows that the current enzymatic hydrolysis technology cannot meet the requirements of industrialization

Although the concentrated patented technology can change the concentration of enzymatically hydrolyzed sugar, the problem of low enzymolysis rate of cellulose has not been solved, which will lead to low utilization rate of raw materials and high production cost.

Patent CN 101230359 discloses a cellulose ethanol process using a modified polydimethylsiloxane polymer membrane to enrich the concentration. It also wants to solve the problems of low fermentation alcohol concentration and high distillation cost, but enzymatic hydrolysis cannot be avoided and the problem of low fermentation efficiency