Method for producing glucose and cellobiose using cellulose complex enzyme

A cellobiose, compound enzyme technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of no economic feasibility, high production cost, low alcohol concentration, etc., and achieve huge industrialization prospects. , The effect of low equipment cost and high saccharification rate

Inactive Publication Date: 2009-12-16
CHINA THREE GORGES UNIV
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Problems solved by technology

The second is that under the condition of high substrate content of 15% (w / w) or more, a large amount of cellulose is not converted, the enzymolysis effect is significantly reduced, and the conversion rate is approximately reduced to a low concentration (5% w / w) Half of the enzymatic efficiency, usually only 40-50%
[0005] At present, domestic patents have reported three processes related to cellulosic ethanol, but the economic feasibility is not strong
Chinese patent CN101130792A discloses a method for preparing fuel ethanol by enzymolysis of stalks. The fermentation time is 10-15 days, and the enzymolysis efficiency is very low, and there is no economic feasibility for industrialization
Chinese patent CN101270372A discloses a method for concentrating sugar liquid in cellulose ethanol fermentation liquid, the purpose is also to solve the problem that the sugar concentration after enzymatic hydrolysis is too low, which shows that the current enzymatic hydrolysis technology cannot meet the requirements of industrialization
Although the concentrated patented technology can change the concentration of enzymatically hydrolyzed sugar, the problem of low enzymolysis rate of cellulose has not been solved, which will lead to low utilization rate of raw materials and high production cost.
Patent CN 101230359 discloses a cellulose ethanol process using a modified polydimethylsiloxane polymer membrane to enrich the concentration. It also wants to solve the problems of low fermentation alcohol concentration and high distillation cost, but enzymatic hydrolysis cannot be avoided and the problem of low fermentation efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Cultivate Trichoderma viride and Aspergillus niger seed solution respectively, and inoculate 6 kg of wheat bran, 25 kg of straw, 1.5 kg of potassium dihydrogen phosphate, 1.8 kg of ammonium sulfate, 0.56 kg of magnesium sulfate, and 0.06 kg of cobalt chloride according to the ratio of 1:1. Tween 80 0.01kg, mixed with 50kg of water in the solid feed liquid. Control the humidity at 60%, and ventilate every 4 hours to ensure sufficient oxygen. After culturing for 5 days, the complex enzyme system was extracted from the solid. The ratio of the obtained compound enzyme system endoglucanase, exoglucanase and β-glucosidase is 7:2:2. In a 1-ton reactor, add 700kg of mixed liquid containing a complex enzyme system, add chemical activator ferric chloride 1.0g, 2g Tween80, 3.5g PEG4000, control the temperature at 50°C, stir at 100rpm, and enzymolysis for 64 hours. The pH value of the hydrolysis is controlled at 4.5-5.0, and the high-performance liquid phase contains 135 g / L of g...

example 2

[0023] Cultivate Trichoderma viride and Aspergillus niger seed solution respectively, and inoculate 10kg of wheat bran, 40kg of straw, 3.5kg of potassium dihydrogen phosphate, 2.3kg of ammonium sulfate, 0.7kg of magnesium sulfate, and 0.16kg of cobalt chloride according to the ratio of 1:2. Tween 80 0.02kg, water 100kg in the mixed solid feed liquid. Control the humidity at 70%, and ventilate every 4 hours to ensure sufficient oxygen. After culturing for 5 days, the complex enzyme system was extracted from the solid. The ratio of the obtained compound enzyme system endoglucanase, exoglucanase and β-glucosidase is 8:1:1. In a 2-ton reactor, add 1200kg of mixed liquid containing a complex enzyme system, add chemical activator manganese chloride 2.4g, 1.6g Tween80, 4.8g PEG6000, control the temperature at 55°C, stir at a speed of 110rpm, and perform enzymolysis for 66 hours. The pH value of enzymatic hydrolysis is controlled at 4.5-5.0, and the content of glucose is 150g / L and ...

example 3

[0025] Cultivate Trichoderma viride and Aspergillus niger seed solution separately, and inoculate 100kg of wheat bran, 350kg of sawdust, 33kg of potassium dihydrogen phosphate, 21kg of ammonium sulfate, 5kg of magnesium sulfate, 1.5kg of cobalt chloride, and 0.3kg of Tween80 according to the ratio of 1:3. , in the mixed solid feed liquid of 1000kg of water. Control the humidity at 70%, and ventilate every 4 hours to ensure sufficient oxygen. After culturing for 5 days, the complex enzyme system was extracted from the solid. The ratio of the obtained compound enzyme system endoglucanase, exoglucanase and β-glucosidase is 5:2:3. In a 1-ton reactor, add 650kg of mixed liquid containing a complex enzyme system, add 1.2g of chemical activators zinc chloride, 1.8g of Tween80, 2.3g of PEG2000, control the temperature at 50°C, stir at a speed of 190rpm, and perform enzymolysis for 72 hours. The pH value of the enzymatic hydrolysis is controlled at 4.5-5.0, and the high-performance l...

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PUM

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Abstract

A method for producing glucose and cellobiose using cellulose complex enzyme is the technology using the cellulose complex enzyme as a catalyst and realizing the high saccharification rate conversion of cellulose by adding cellulose enzyme activator at the optimized stirring speed. Glucose concentration can reach 150g/L after using cellulose enzymatic hydrolysis, and the method is a cellulose saccharification production technology with economic feasibility. With the technology used, low cost, low usage of enzyme and high saccharification rate can be realized, and the productions of cellulose alcohol and biochemical products (lactic acid, succinic acid and the like) are satisfied. In the whole technology, no beta-glucosaccharase is added additionally, no sugar concentrating device is added, and the technology is simple, the equipment cost is low and the industrialized prospect is good.

Description

technical field [0001] The invention relates to a high-efficiency production method of cellulose saccharification process, in particular to a method for saccharifying cellulose by using a compound cellulosic enzyme system and a chemical activator. Background technique [0002] With the increasing depletion of petroleum resources, the increasing shortage of food, and the huge demand for liquid fuels such as ethanol and butanol and other biomass chemical products (lactic acid, succinic acid, etc.), the use of lignocellulose as raw material The research and development of producing fermentable sugar and then biotransforming biochemical products has become a hot spot in the scientific and industrial circles of China, the United States and other world energy-consuming countries. [0003] my country is a large agricultural country, and about 700 million tons of agricultural waste is formed every year, including 110 million tons of wheat straw, 180 million tons of rice straw, 13 mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/16C12P19/14C12P19/12C12P19/02C12R1/685C12R1/885
Inventor 龚大春汤金婷李志军牛涛曾晶俞学峰
Owner CHINA THREE GORGES UNIV
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