Bifidobacterium pseudocatenulatum CCFM1046, composition, fermented food, applications, microbial inoculum and preparation method thereof

A bifidobacterium and fermented food technology, applied in the field of microorganisms, can solve problems such as irritating the gastrointestinal tract, liver function damage, and affecting patients, and achieve the effects of alleviating weight gain, improving weight gain, and reducing Parkinson's disease

Active Publication Date: 2019-08-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These drugs have a good curative effect on each single risk factor of metabolic syndrome. However, long-term use of drugs has certain side effects. For example, long-term use of metformin can cause irritat

Method used

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  • Bifidobacterium pseudocatenulatum CCFM1046, composition, fermented food, applications, microbial inoculum and preparation method thereof
  • Bifidobacterium pseudocatenulatum CCFM1046, composition, fermented food, applications, microbial inoculum and preparation method thereof
  • Bifidobacterium pseudocatenulatum CCFM1046, composition, fermented food, applications, microbial inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1: Pseudo-small chain Bifidobacterium CCFM1046 has good tolerance to simulated gastrointestinal fluid

[0085] Inoculate the cryopreserved Pseudomonas bifidobacterium CCFM1046 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and then subculture in mMRS medium for 2 After ~3 times, take 1mL of the culture solution of Bifidobacterium pseudospermia CCFM1046, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and anaerobically For culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultured with mMRS agar medium to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0086]The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the time of 0h in the culture fluid, expres...

Embodiment 2

[0091] Example 2: Bifidobacterium pseudosmall-chain CCFM1046 has no toxic or side effects on C57BL / 6J mice. Bifidobacterium pseudo-small-chain CCFM1046 bacteria were resuspended in 3% sucrose solution to make a concentration of 3.0 × 10 8 CFU / mL bacterial suspension. Get 6 healthy male C57BL / 6J mice with a body weight of about 20g. After acclimating to the environment for a week, give the bacterial suspension of this concentration orally once a day, observe for a week, and record the death and body weight.

[0092] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The pseudo small chain bifidobacteria CCFM1046 of CFU / mL did not cause significant impact on mice, and the body weight had no significant change, and no death phenomenon occurred. The appearance of the mice had no obvious pathological symptoms.

[0093] Table 3 Changes in body weight and death of mice

[0094]

[0095] Note: -: no death of mice

Embodiment 3

[0096] Embodiment 3: Pseudosmall chain Bifidobacterium CCFM1046 has a recovery effect on the intestinal flora imbalance caused by high-fat feed

[0097] 36 healthy male C57BL / 6J mice weighing 18-20 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-fat model control group (HFD), simvastatin control group (SC ), the rosiglitazone control group (RH), the Bifidobacterium pseudobacterium CCFM1046 intervention group (CCFM1046), the Bifidobacterium animalis BB12 control group (BB12), each group contained 6 mice, and the bacteria suspension The dose is 3.0 x 10 8 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:

[0098] Table 4 Grouping of experimental animals

[0099]

[0100] The body weight of the mice was monitored regularly during the experiment.

[0101] At the end of the experiment, the fresh feces of the mice were collected an...

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Abstract

The invention discloses bifidobacterium pseudocatenulatum CCFM1046, a composition, a fermented food, applications, a microbial inoculum and a preparation method thereof. As the bifidobacterium pseudocatenulatum CCFM1046 can tolerate gastrointestinal environments in human bodies, the rising of hepatic triglyceride content of metabolic syndrome mice caused by high-fat diet can be significantly improved; the weight increasing of the metabolic syndrome mice can be alleviated; the abnormal fasting blood glucose and oral glucose tolerance of the metabolic syndrome mice can be significantly improvedas well; area under the curve of glucose tolerance can be reduced; serum insulin levels can be enhanced; the rising of low-density lipoprotein cholesterol in serum of the metabolic syndrome mice caused by the high-fat diet can be significantly improved; and the rising of Proteus genus in intestinal tracts caused by the high-fat diet can also be significantly improved. The bifidobacterium pseudocatenulatum CCFM1046 can be used for preparing the pharmaceutical composition and fermented food for alleviating metabolic syndromes and decreasing the occurrence of Parkinson, intestinal infection, urinary tract and kidney infection and other diseases, so that the bifidobacterium pseudocatenulatum CCFM1046 can have very wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Bifidobacterium pseudosmall chain CCFM1046, its composition, fermented food, application, bacterial agent and preparation method thereof. Background technique [0002] In recent years, with the continuous development of my country's social and economic level, the living standards of the public have continued to improve, the proportion of carbohydrates and fats in the diet structure has increased day by day, and the restrictions of many working conditions have made it easy for people to develop sedentary behavior Habit. These external environmental factors combined with their own genetic factors have jointly led to the continuous increase in the incidence of metabolic syndrome in the Chinese population. According to reports, between 2002 and 2010, the proportion of people over the age of 20 in China suffering from metabolic syndrome rose from 5.4% to 21.3%, an increas...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/123A23C13/16A23C19/032A23C11/10A23L11/00A23L33/00C12R1/01A23L11/50A23L11/60
CPCC12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/1234A23C13/16A23C19/0323A23C11/106A23L33/00A61K2035/115A23V2002/00A23L11/50C12R2001/01C12N1/205A23V2400/535A23V2200/3204A23V2200/3262A23V2200/328A23V2200/32A23V2200/322A23V2200/30
Inventor 陈卫王刚刘煜飞赵建新张灏
Owner JIANGNAN UNIV
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