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Enzymatic triglyceride measuring method and measuring reagent

A technology of triglyceride and determination method, applied in the field of medical inspection, can solve the problems of reagent accuracy, poor precision, low absorption signal, instability and the like, and achieve the effect of good precision

Inactive Publication Date: 2012-10-03
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this scheme, the free glycerol and the glycerol produced by the hydrolysis of TG are successively colored. The instrument uses the quinone imine produced in the first step as a blank, and only uses the quinone imine produced in the second step to calculate the TG content, so as to remove the free glycerin. However, due to the low and unstable absorption signal produced by free glycerol, the reagent accuracy and precision of this method are poor, and the repeatability of clinical results is not good, which cannot meet the requirements of routine testing.

Method used

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  • Enzymatic triglyceride measuring method and measuring reagent
  • Enzymatic triglyceride measuring method and measuring reagent
  • Enzymatic triglyceride measuring method and measuring reagent

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Experimental program
Comparison scheme
Effect test

Embodiment

[0039] Reagent 1:

[0040] Reagent 2:

[0041] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0043] Reagent 1:

[0044]

[0045] Reagent 2:

[0046] The preparation method of embodiment 2 reagent is the same as embodiment 1.

Embodiment 3

[0048] Reagent 1:

[0049] Reagent 2:

[0050]

[0051] The preparation method of embodiment 3 reagent is the same as embodiment 1.

[0052] The test conditions for the reagent of the present invention to measure TG in a sample are as follows: temperature: 37° C.; light path of cuvette: 1.0 cm. The main detection wavelength is 546nm, and the secondary wavelength is 700nm.

[0053] The method of measuring TG in the sample by using the TG determination reagent of the present invention is as follows: add R1 to the sample (calibration tube as the sample) and mix evenly, incubate at 37°C for 5 min, add R2 and mix evenly, record the absorbance A1, and react at 37°C for 5 min After that, record the absorbance A2. The sample volume is 3 μl, the reagent 1 volume is 200 μl, and the reagent 2 volume is 100 μl.

[0054] TG content in the reagent measurement sample of the present invention is calculated according to the following formula:

[0055]

[0056] Obtain the concentra...

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Abstract

The invention discloses a method for measuring triglyceride content of a sample by eliminating H2O2 produced by free glycerol through catalase in a reagent 1 of an enzymatic triglyceride measuring reagent and inhibiting the catalase in the reagent 1 through a catalase inhibitor in a reagent 2. According to the method, the H2O2 produced by the free glycerol is eliminated by adopting the catalase, and produced water and oxygen do not increase the cost; the catalase in the reagent is completely inhibited by the inhibitor during detection reaction, so that influence on the reaction is avoided; the method can solve the problems of increased instable background and low detection result accuracy and precision when the H2O2 is oxidized by peroxidase; and the method has the advantages of both high accuracy and high precision.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an enzymatic triglyceride assay method and assay reagents. Background technique [0002] Triglyceride (Triglyceride, TG) is a fat molecule formed by long-chain fatty acids and glycerol. It is the most abundant lipid in the human body. Most tissues can use TG decomposition products to supply energy. At the same time, tissues such as liver and fat can also carry out TG synthesis and storage in adipose tissue. [0003] The Laboratory Branch of the Chinese Medical Association recommended a two-step enzymatic method in 1995 as a routine method for the determination of serum TG. This method uses lipoprotein lipase (LPL) to hydrolyze TG to generate glycerol and fatty acids; GK) and glycerol phosphate oxidase (glycerol-3-phosphate oxidase GPO) catalyze the generation of H 2 o 2 , under the catalysis of peroxidase (POD), H 2 o 2 , 4-aminoantipyrine (4-AAP) and 2,4-dichlorophe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 邹炳德邹继华沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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