74 results about "Glycerol phosphate" patented technology

The invention relates to the field of gene engineering, and discloses a D-3-phosphoglycerate dehydrogenase mutant, a coding gene thereof and application of the D-3-phosphoglycerate dehydrogenase mutant and the coding gene. Compared with a protein with an amino acid sequence as shown in SEQ ID NO: 1, the mutant comprises mutation at the 131 site and / or the 285 site. The mutant can specifically catalyze a substrate 3-phosphoglycerate to synthesize 3-phosphate-hydroxypyruvic acid, can effectively promote synthesis of L-serine in a metabolic pathway of microorganisms, improves the yield and conversion rate of the L-serine, and reduces the yield of byproducts at the same time.

The invention discloses a yarrowia lipolytica engineering strain with high yield of resveratrol as well as construction and application of the yarrowia lipolytica engineering strain, and belongs to the technical field of synthetic biology and metabolic engineering. The invention provides a yarrowia lipolytica engineering strain. A 4-coumaric acid-CoA ligase gene, a multi-copy resveratrol synthase gene, a transketolase 1 gene, a fusion gene of a histidine phosphate aminotransferase gene and a tyrosine ammonia lyase gene, a glycolytic pathway hexokinase gene, a 6-phosphofructokinase-1 gene and a phosphoglycerate kinase gene are integrated, so that the recombinant protein is obtained. According to the present invention, by using glucose as the substrate, the high yield of resveratrol can be achieved by using glucose as the substrate, the resveratrol yield during shake-flask culture fermentation can achieve 3.157 g / L, the resveratrol yield can be increased to 30.7 g / L during 5L-scale fed-batch fermentation, and the resveratrol yield can be increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L; the maximum value is reported by the yarrowia lipolytica system at present.

The invention discloses a composite dispergation preparation for catalyzing resin degradation and a preparation method and application thereof, the composite dispergation preparation for catalyzing resin degradation comprises a composite bio-enzyme preparation and an auxiliary agent; the composite biological enzyme preparation comprises 3-phosphoglycerol dehydrogenase and high-temperature citric acid synthetase. According to the process method for degrading the waste glass steel slag by using the composite peptizing preparation, a catalytic reaction process is adopted, and low-temperature, low-pressure and normal-temperature reaction conditions are adopted, so that the energy consumption is reduced; the reaction time is short, three wastes (waste gas, wastewater and waste residue) are avoided, and water in the whole system is completely recycled; the harmless and resource utilization of the treated waste residues is ensured; and various resins (epoxy resin, phenolic resin, polyacrylamide formaldehyde resin and the like) can be degraded.

The invention provides an osteogenic induced differentiation kit, a culture medium and a preparation method of the culture medium, and particularly relates to the technical field of stem cells. The kit comprises a DMEM (dulbecco's modified eagle medium) basal culture medium, fetal calf serum, dexamethasone, vitamin C, beta-glycerol phosphate, mesenchymal stem cell osteogenesis induced supernatant, alizarin red S staining solution and acetic acid. According to the kit provided by the invention, the problems of slow calcium nodule formation, small calcium nodule quantity and small calcium nodule in the osteogenic induced differentiation of the umbilical cord mesenchymal stem cells in the prior art are solved, so that the calcium nodule formation of the umbilical cord mesenchymal stem cells is effectively promoted, meanwhile, the calcium nodule quantity can be increased, and the osteogenic induced differentiation period is shortened.

The present invention relates to a non-invasive test for the detection of deep endometriosis. Particularly, although not exclusively, aspects of the present invention relate to one or more glycolysis-associated biomarkers (e.g., phosphoglycerate mutase 1 (PGAM1), Enolase-1 (ENO1), phosphofructokinase-1 (PFKP) and / or Hexokinase) and / or cytokines associated with aerobic glycolysis (e.g., IL-6 and / or TGF-β) which can be used to determine the likelihood of a subject having deep endometriosis based on a sample obtained from the subject.

The application discloses a duck Riemerella anatipestifer (RA) surface adhesion factor 3-glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) conservative B cell epitope peptide, a nucleic acid molecule, a recombinant carrier and application thereof. The application inserts the conservative B cell epitope TG12 into chicken white dysentery Salmonella Peg fimbria, introduces the inert carrier bacteria, and obtains the recombinant bacteria which can functionally express and present the RaGAPDH protein surface conservative B cell epitope peptide. The expression and presentation of the conservative B cell epitope can specifically recognize and combine duck and goose RA infection serum, and the naked-eye visible agglutination reaction particles are observed, and there is no cross agglutination reaction with other pathogen infection positive serum. The agglutination detection method has the advantages of specificity and convenience, and is expected to provide a new idea and method for detection and prevention and control of RA infection.

The present invention relates to a process for producing 4-hydroxymethylfurfural (HMF) comprising the steps of: converting 4-hydroxymethylfurfural-phosphate to 4-hydroxmethylfurfural using a phosphatase; and a process for producing 4-HMF from a feedstock comprising a carbon source, comprising the steps of converting 4-hydroxymethylfurfural-phosphate to 4-hydroxmethylfurfural using a phosphatase and an enzyme cascade comprising i.a. synthases to convert fructose-6-phosphate to glyceraldehyde-3-phosphate; wherein the carbon source is preferably selected from the group consisting of starch, dextrines, cellulose, sucrose and / or combinations thereof.

The invention provides recombinant Escherichia coli for synthesizing heme and derivatives thereof, and the recombinant Escherichia coli is used for knocking out genes such as pyruvate oxidase poxB, pyruvate formate lyase pflB and porphyrinoperoxidase yfeX and enhancing genes such as phosphoenolpyruvate carboxylase ppc, glycerol transporter glpF and 3-phosphoglycerol dehydrogenase glpD, so that the heme and the derivatives thereof can be synthesized. After genes such as exogenous 5-aminolevulinic acid synthase hemA and ferrous chelating enzyme hemH are introduced, the heme and the derivatives thereof can be synthesized by taking glycerol as a raw material, and the heme has an application prospect in the field of biological manufacturing.

Recombinant microorganism in which a gene encoding a transcriptional repressor of the glycine cleavage system, pyruvate formate lyase or phosphoglycerate dehydrogenase, is attenuated or deleted from a host microorganism with a formic acid assimilation pathway, a ppsR gene encoding a phosphoenolpyruvate synthase regulatory protein, or a purT gene encoding a phosphoribosylglycinamide formyltransferase, is attenuated or deleted, a gcvTHP gene, consisting of the gcvT gene, the gcvH gene and the gcvP gene, which encodes an enzyme involved in a glycine cleavage system reaction in which host microorganism with the formic acid assimilation pathway is highly expressed, and a gene encoding formate tetrahydrofolate ligase, methenyl tetrahydrofolate cyclohydrolase or methylene tetrahydrofolate dehydrogenase is introduced into the host microorganism via the formic acid assimilation pathway.

The invention provides a composite positive plate, a preparation method thereof and a sodium ion battery, and relates to the technical field of batteries. The composite positive plate provided by the invention comprises a current collector and a positive active material layer coated on the current collector, the positive electrode active material layer comprises a positive electrode main material, a conductive agent and a binder; the positive electrode main material comprises composite ferric phosphate sodium and an O3-phase layered oxide; the mass ratio of the composite sodium ferric phosphate to the O3-phase layered oxide is (1: 1)-(9: 1). The composite positive plate is large in compaction density and high in gram volume; the surface of the composite sodium ferric phosphate is coated with glycerophosphate, the specific surface area of the composite sodium ferric phosphate can be reduced, the material is not prone to agglomeration due to the negative electricity characteristic of the glycerophosphate, meanwhile, compared with the composite sodium ferric phosphate, glycerin groups have more affinity with a solvent, the solvent can fully wet the surface of the material, and the surface of the material is more stable. The final solid content of system homogenate is further improved, and the solvent loss is reduced.

The invention discloses a titanium oxide modified graphite phase carbon nitride nano-composite plugging agent and an oil-based drilling fluid. The titanium oxide modified graphite phase carbon nitride nanosheet composite blocking agent is prepared from the following synthetic raw materials: melamine, tetrabutyl titanate, phosphorous acid, glycerol, ethanol, ethylene glycol, an initiator and deionized water. The drilling fluid comprises the titanium oxide modified graphite phase carbon nitride nano composite plugging agent provided by the invention. The particle size distribution of the titanium oxide modified graphite phase carbon nitride nano-composite blocking agent is 350-620 nm, nano-sized pores in a shale well wall can be effectively blocked, so that the well wall can be effectively stabilized, collapse can be prevented, and meanwhile, the preparation method is reliable in principle and has a wide market prospect; the oil-based drilling fluid used in the invention has good rheological property, stability, plugging property and other properties under shale formation conditions.

This invention belongs to the field of bioengineering, specifically disclosing a 3-phosphoglycerate dehydrogenase mutant with improved enzyme activity and thermostability. Compared to the wild-type 3-phosphoglycerate dehydrogenase from *Corynebacterium glutamicum*, the mutant contains one mutation from among P85Q, D365Y, A389T, M196V, A183V, and I231V. Enzyme activity assays after induced expression and purification showed that the mutant's enzyme activity was 1.5–3.6 times higher than the wild type, and its thermostability was significantly improved. Therefore, the beneficial mutant provided by this invention lays a good foundation for the industrial fermentation production of L-serine and L-cysteine.

The present invention discloses an antibacterial compound of Formula 1 or pharmaceutically acceptable salt thereof:wherein X is CH, S, CH—NH2;R1 is C1-C8 alkyl, substituted alkyl, alkyl amine, substituted amine; preferably R1 is methyl, ethyl, propyl, butyl, pentyl, isopropyl, isobutyl, methyl amine, ethylamine, propylamine, isopropyl amine, isobutylamine, N-ethylprop-2-yn-1-amine, N-isopropyl propane-1,3-diamine, N1-isopropyl ethane-1,3-diamine, 1-butyl-2-methylguanidine, N1-ethyl-N1-propylethane-1,2-diamine, cyclobutyamine, phosphate, sulphate;R2 is hydrogen, alkyl, substituted alkyl; preferably R2 is methyl, propyl, isopropyl;R3 is hydrogen, alkyl, substituted alkyl; and preferably R3 is methyl, propyl, isopropyl.The Formula 1 or pharmaceutically acceptable salt thereof is an imidazole glycerol phosphate dehydratase (IGPD) inhibitor and treats or prevents or ameliorates tuberculosis.

The invention provides glycoprotein phosphoglycerate kinase derived from stropharia rugoso-annulata, a recombinant vector, recombinant pichia pastoris engineering bacteria and application of the glycoprotein phosphoglycerate kinase, and belongs to the technical field of phosphoglycerate kinase production. The phosphoglycerate kinase S.rug.-PGK sourced from S.rug.-PGK provided by the invention has an amino acid sequence as shown in SEQ ID NO. 1, and a nucleotide sequence for coding the S.rug.-PGK is as shown in SEQ ID NO. 2. According to the invention, novel phosphoglycerate kinase (S.rug.-PGK) derived from Stropharia rugoso-annulata is taken as a target, and efficient secretion production of S.rug.-PGK is realized by systematically optimizing a pichia pastoris expression platform. According to the invention, efficient secretory expression of S.rug.-PGK in pichia pastoris is realized for the first time, it is clear that the pichia pastoris X33 strain is an optimal production system under the conditions that OD600 is equal to 2 and the inducer concentration is 1%, and a foundation is laid for large-scale preparation.

A set of serum metabolic biomarkers and detection kit for detecting drug-resistant tuberculosis are provided. The set of the serum metabolic biomarkers includes 17 serum metabolic biomarkers. The 17 serum metabolic biomarkers are verified to be associated with tuberculosis based on the level changes of these biomarkers. The 17 metabolites includes taurine, homocysteine, uric acid, ascorbic acid, suberylglycine, uridine, dopamine 4-sulfate, inosinic acid, glyceraldehyde phosphate, [3-methoxy-4-(phosphoryl)phenyl]carbonyl sulfonic acid, nuclomedone, n4-cyclopropyl-6-(2,3-dichlorophenyl)-1,2,3,4-tetrahydropyrimidine-2,4-diimine, 1-[2-chlorine-2-(2,4-dichlorophenyl)ethenyl]-1,2,4-triazole, tetrachloro-phthalic anhydride, malotilate, fulvic acid, and L-neopterin. The serum metabolic biomarkers and detection kit for detecting drug-resistant tuberculosis can assist doctors in accurately diagnosing the disease, which is of great significance for the diagnosis and mass screening for drug-resistant tuberculosis.

Genetically modified yeast cell of the genus Saccharomyces with improved glycerol catabolism, wherein the yeast cell is genetically modified such that a) the degradation of glycerol to dihydroxyacetone phosphate via the glycerol-3-phosphate pathway is blocked by the deletion of the genes for glycerol kinase (GUT1) and / or mitochondrial glycerol-3-phosphate dehydrogenase (GUT2), and b) a heterologous glycerol uptake facilitator protein is expressed, wherein the heterologous glycerol uptake facilitator protein is Fps1 or Fps2, and c) a heterologous glycerol dehydrogenase catalyzing the oxidation of glycerol to dihydroxyacetone is expressed or a heterologous glycerol dehydrogenase catalyzing the oxidation of glycerol to dihydroxyacetone is overexpressed.or c2) at least one enzyme involved in the degradation of glycerol to glyceraldehyde-3-phosphate via the glyceraldehyde pathway is overexpressed, or at least one enzyme involved in the degradation of glycerol to glyceraldehyde-3-phosphate via the glyceraldehyde pathway is replaced by a heterologous enzyme with the same enzyme activity.

The object of the present invention is a liposomal composition comprising the tripeptide glycyl-L-histidyl-L-lysine-copper (GHK-Cu) complex, wherein said complex is encapsulated in lipid carrier A comprising 19 to 39 wt % of the sodium salt of 1,2-distearoyl-sn-glycero-3- phosphoglycerol (DSPG-Na), 29 to 42 wt % of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 24 to 41 wt % of cholesterol, or in lipid carrier B comprising 16 to 21 wt % of N- [carbonyl-methoxy(polyethylene glycol)-2000]-1,2-distearoyl-sn-glycero-3- phosphoethanolamine (DSPE-PEG2000), 48 to 63 wt % of hydrogenated soybean phosphatidylcholine (HSPC) and 16 to 36 wt % of cholesterol. A further object of the invention is a method for preparing the liposomal composition of the invention and its use as a component of a cosmetic formulation.

The invention relates to the technical field of genetic engineering, in particular to a recombinant bacterium for high-yield serine and application of the recombinant bacterium in fermentation production of serine. The ptsI gene of escherichia coli is knocked out, a glucose PTS system is blocked, glucose transport and phosphorylation and PEP-pyruvic acid associated reaction are relieved, and pyruvic acid is synthesized by reducing serine synthesis precursor 3-phosphoglyceric acid branches. Growth adaptive evolution is further carried out on escherichia coli with the ptsI gene knocked out, an obtained mutant strain is sequenced, the mutation condition is shown in a table 2, and finally the growth rate accelerating chassis bacterium is obtained. Afterwards, by enhancing the expression serine key genes serA, serB and serC of the mutant strain and knocking out serine degradation pathway genes sdaA, sdaB and tdcG, the engineering bacterium for fermentation production of serine is constructed, and the serine yield of the engineering bacterium is remarkably improved.

The invention provides an umbilical cord mesenchymal stem cell osteogenesis induced differentiation culture medium and a preparation and induction method, and particularly relates to the technical field of stem cells. The culture medium comprises a Dulbecco's Modified Eagle Medium (DMEM) basal culture medium, and further comprises the following components in concentration or proportion: 5-15% by volume of fetal calf serum, 0.1-10 [mu] M of dexamethasone, 10-450 [mu] g / ml of vitamin C, 2-80 mM of beta-glycerol phosphate, and 10-250 [mu] g / ml of tricalcium silicate doped with magnesium, zirconium and zinc ion components. According to the umbilical cord mesenchymal stem cell osteogenic induction differentiation culture medium provided by the invention, the problems of slow calcium nodule formation, small calcium nodule quantity and small calcium nodule in the osteogenic induction differentiation of umbilical cord mesenchymal stem cells in the prior art are solved, so that the calcium nodule formation of the umbilical cord mesenchymal stem cells is effectively promoted, meanwhile, the calcium nodule quantity can be increased, and the survival rate of the umbilical cord mesenchymal stem cells is increased. And the osteogenic induced differentiation period is shortened.

The invention provides a genetically engineered recombinant Klebsiella michiganensis having reduced or inactivated glucose-specific PTS transport system, glycerol kinase, glycerol dehydrogenase, alcohol dehydrogenase, acetolactate synthase, acetolactate decarboxylase, fumarate reductase and 3-phosphoglyceraldehyde dehydrogenase, having, preferably enhanced, NADPH-dependent alcohol dehydrogenase, diol dehydratase, 3-phosphoglycerate dehydrogenase and 3-phosphoglycerate phosphatase activities, methods of making said recombinant Klebsiella michiganensis, and uses and methods of producing 1,3-propanediol using said recombinant Klebsiella michiganensis.

This invention relates to the field of shielding coating technology, and more particularly to a shielding coating and its preparation method. The shielding coating is composed of the following raw materials in parts by weight: 30-40 parts coating resin, 5-15 parts magnetic porous conductive powder, 6-8 parts curing agent, 1-3 parts coupling agent, 0.3-0.6 parts modified polyurethane block copolymer dispersant, 0.05-0.2 parts coating drying agent, 10-15 parts solvent, 0.8-2 parts terminal amine polyol ester, and 2-4 parts sodium glycerophosphate. The shielding coating proposed in this invention incorporates magnetic porous conductive powder, which contains nano-ferric oxide. The nano-ferric oxide attracts each other, resulting in small gaps between the magnetic porous conductive powder particles. After curing, the magnetic porous conductive powder particles form a dense film, effectively ensuring the shielding effect of the coating.

This invention belongs to the technical field of creatine kinase detection kits, specifically relating to a reagent, kit, and application for detecting creatine kinase, including a buffer, creatine phosphate, glycerol, glycerol kinase, glycerol phosphate oxidase, peroxidase, and a chromogenic agent, and also containing a CK activating reducing agent, as well as perfluorinated and polyfluoroalkyl substances. The CK activating reducing agent is a sulfur-containing reducing agent. This invention can improve the accuracy of detection.

The present disclosure concerns recombinant microbial host cell having, in glycolytic conditions, increased glycerol importing activity glycerol as well as, in high osmotic conditions, a decreased NAD-dependent glycerol-3-phosphate dehydrogenase (GPD) activity. The recombinant microbial host cell is particularly useful for the fermentation of sugarcane- or molasses-based medium for the production of ethanol.

The invention discloses a multifunctional fluorosilicone biolipid nano oil-displacing agent, which comprises 20-40% of fluorosilicone surface active nano fluid, 10-20% of a surfactant, 10-20% of a surfactant, and the balance of water. 5%-10% of biolipid; and the balance of water. Wherein the fluorosilicone surface active nanofluid is prepared by grafting and modifying a modified fluorocarbon glycerophosphate intermediate and nanosilicon dioxide. The oil-displacing agent disclosed by the invention has a nano-scale'seepage-displacing-washing 'integrated multifunctional effect, is excellent in temperature resistance, salt tolerance, oil washing efficiency and imbibition oil-displacing capacity, is suitable for low-permeability oil reservoirs with the temperature of 140 DEG C and the degree of mineralization of 150000mg / L, has the comprehensive capacity improved by more than 30% compared with that of an imbibition oil-displacing agent used on site, and has a good application prospect. The multifunctional fluorosilicone biolipid nano oil displacement agent has wide application prospects in hydrofracture of low-permeability reservoirs and later yield increasing and stabilizing processes.

The invention provides a chimeric recombinant vector containing SCW10 signal peptide, recombinant pichia pastoris engineering bacteria and application of the recombinant pichia pastoris engineering bacteria in expression of glycoprotein S.rug.-PGK, and belongs to the technical field of phosphoglycerate kinase production. According to the chimeric recombinant vector containing the SCW10 signal peptide, the chimeric recombinant vector is obtained by chimerizing the N end of the SCW10 signal peptide and the C end sequence of alpha-factor signal peptide in the recombinant vector, and the nucleotide sequence of the SCW10 signal peptide is as shown in SEQ ID NO.1; the recombinant vector is a recombinant vector containing phosphoglycerate kinase S.rug.-PGK from Stropharia rugoso-annulata, and the amino acid sequence of the S.rug.-PGK is as shown in SEQ ID NO. 2. The chimeric recombinant vector containing the SCW10 signal peptide constructed by the invention can be successfully transformed into pichia pastoris, so that the purpose of efficiently secreting and producing a large amount of PGK by the pichia pastoris is achieved.

The present application provides a recombinant E. coli for synthesizing heme and derivatives thereof, wherein the recombinant E. coli is knocked out of genes such as pyruvate oxidase poxB, pyruvate formate-lyase pflB and protoporphyrinogen peroxidase yfeX, and meanwhile, the genes such as phosphopyruvate carboxylase ppc, glycerol transporter glpF and 3-phosphoglycerate dehydrogenase glpD are enhanced, and the genes such as 5-aminovaleryl-CoA synthase hemA and ferrous chelatase hemH are introduced, so that the recombinant E. coli can synthesize heme and derivatives thereof by using glycerol as a raw material, and has application prospects in the field of biological manufacturing.

A liposome encapsulating adenosine is provided herein. Liposomes can be formed by a combination of sphingomyelin or sphingomyelin and 1,2-dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) or a combination of sphingomyelin and 1,2-dimyristoyl-sn-glycerol-3-phosphoglycerol (DMPG) or a combination of sphingomyelin, DMPG and DMPC. Liposomes encapsulating adenosine can be used to induce cartilage regeneration, treat osteoarthritis, relieve joint pain, and / or slow down, prevent and / or reverse the progressive structural tissue damage associated with osteoarthritis, or treat osteoarthritis, rheumatoid arthritis, acute gouty arthritis and / or synovitis. Liposomes can release adenosine for up to two weeks.

The application discloses a D-3-phosphoglycerate dehydrogenase mutant and application thereof in improving L-tryptophan, and belongs to the technical field of biology.The technical problem to be solved by the application is how to improve the yield of L-tryptophan.Based on this, the application provides the D-3-phosphoglycerate dehydrogenase mutant and application thereof in improving the yield of L-tryptophan, wherein the D-3-phosphoglycerate dehydrogenase mutant is a protein with an amino acid sequence comprising SEQ ID NO:3.The application can be used for improving the yield of L-tryptophan of Escherichia coli.

The invention discloses a D-3-phosphoglycerate dehydrogenase mutant and application of the D-3-phosphoglycerate dehydrogenase mutant in improving L-tryptophan, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to improve the yield of L-tryptophan. On the basis, the invention provides a D-3-phosphoglycerate dehydrogenase mutant and an application of the D-3-phosphoglycerate dehydrogenase mutant in improving the yield of L-tryptophan, and the D-3-phosphoglycerate dehydrogenase mutant is a protein of which the amino acid sequence comprises SEQ ID NO: 3. The method can be used for improving the yield of the L-tryptophan of the Escherichia coli.

This invention provides a multi-enzyme cascade catalytic reaction system and its application, belonging to the field of enzyme catalysis production technology. Using glycerol as a raw material, it includes a first module, a second module, and a third module. The first module involves the production of glyceric acid from glycerol under the catalysis of alditol oxidase. The second module contains glyceric acid, ATP, and NAD+. + L-glutamic acid is synthesized into phosphoserine via glycerate kinase, D-3-phosphoglycerate dehydrogenase, and phosphoserine transaminase. The third module involves phosphoserine and nucleophiles being catalyzed by phosphoserine thioylase to synthesize L-cysteine ​​or a non-natural L-α-amino acid. The multi-enzyme cascade catalytic reaction system provided by this invention can catalyze a variety of nucleophiles, yields high products, is low in cost, causes minimal environmental pollution, and is easily industrialized.