4 results about "Pyruvic dehydrogenase" patented technology

The application discloses a kind of modified bacteria for producing L-alanine, compared with wild type bacteria, the genome is integrated with pyruvate dehydrogenase alaD coding gene of bacillus stearothermophilus origin and the coding gene of mutant alaE (A48S / A149D) of alanine export protein alaE, and the expression of gene ldhA, mgsA, pflB, adhE, ackA and frdB in chassis bacterial genome is interrupted.The application also discloses a kind of method for quickly constructing recombinant escherichia coli for producing L-alanine, the method is suitable for multiple escherichia coli subspecies including MG1655, BL21 and ATCC8739, has universality and popularization and application value.

The present invention relates to recombinant Modified Vaccina Virus Ankara (MVA) encoding Epstein-Barr virus (EBV) antigens, wherein surface glycoprotein 350 (EBV gp350) and glycoprotein gH (EBV gH) are fused to subunits of self-assembling multimeric protein particle PdhC (acetyltransferase of pyruvate dehydrogenase (PDH) complex) or DPS (DNA binding protein from starved cells).

This invention relates to the field of agricultural biotechnology, specifically to a method and strain for constructing an engineered strain of *Hookworm Copper-Gropping Bacteria* capable of producing methylmalic acid using both organic and inorganic carbon sources. This application involves knocking out the restriction endonuclease encoding gene of the type I restriction modification system in the genome of wild-type *Hookworm Copper-Gropping Bacteria* H16. 、 Promoters adapted for methylmalate synthase expression in *Hypoxonometra* were screened, and methylmalate synthases with high catalytic activity in *Hypoxonometra* were obtained, capable of effectively catalyzing methylmalate synthesis in *Hypoxonometra*. A methylmalate transporter protein that promotes methylmalate excretion was also screened, as well as a gene encoding the *Hypoxonometra* endogenous pyruvate dehydrogenase complex subunit that promotes methylmalate synthesis when sodium gluconate is used as a carbon source.

Provided herein is a method of treating a patient having pyruvate dehydrogenase complex deficiency (PDCD), including administering to the patient an amount of triheptanoin effective to treat PDCD in the patient.