The invention belongs to the technical field of
biotechnology and
protein determination, and particularly relates to confining liquid as well as a preparation method and application thereof. Modified
bovine serum albumin and recombinant
casein polypeptide are used as core components of the confining liquid, hydroxypropyl-beta-
cyclodextrin, polyglycerol
fatty acid ester and other auxiliary components are added, and the confining liquid is prepared through a specific technology. The modified
bovine serum albumin and the recombinant
casein polypeptide have a synergistic effect, so that active sites of the detection carrier can be efficiently sealed; the stabilizing agent and other components are supplemented, so that the stability and compatibility of the confining liquid are improved. The confining liquid is simple and convenient in preparation process, controllable in cost, suitable for various detection carriers such as elisa plates and
nitrocellulose membranes, capable of reducing non-specific binding and improving detection sensitivity, and suitable for various biological detection scenes such as
enzyme-linked immunosorbent
assay.