Application of Chinese yarn glycoprotein in improvement of activities of oxidative metabolism enzymes of mitochondria of brain cell

A technology of yam sugar and mitochondria, which is applied in the direction of peptide/protein components, medical preparations containing active ingredients, drug combinations, etc., can solve problems that have not been seen before, and achieve the effects of safe and convenient use, significant curative effect, and sufficient drug sources

Active Publication Date: 2013-02-13
新乡博凯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Yam glycoprotein is a natural active ingredient contained in the rhizome of the yam plant of the genus Dioscorea in the Dioscoreaceae family. Previously published studies have proved that this substance can effectively treat sleep apnea syndrome, and has anti-hypoxia and anti-fatigue effects (ZL201010171536 .1 and ZL2010101715337), the present invention further studies on this basis and shows that: yam glycoprotein can significantly improve the activity of pyruvate dehydrogenase and ɑ-ketoglutarate dehydrogenase in the brain cell mitochondria, so that the ischemia, The damage and dysfunction caused by hypoxia and other factors caused by the reduction of mitochondrial oxidative metabolism in brain cells have been treated and recovered, but so far, there have been no relevant reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the preparation of yam glycoprotein

[0014] Take 1000g of yam raw medicine and crush it into fine powder, add 2 times the amount of water to heat, the temperature is 50°C, extract twice, each time for 2 hours, filter and combine the two extracts, concentrate the extract to 50%, and the concentration to 95 Add % ethanol to the concentrated solution to make the alcohol content reach 60%. After the white precipitate appears, let it stand for 3-6 hours, separate the precipitate from the supernatant, and continue to add 95% ethanol to the supernatant to make it Its alcohol content reaches 90%, and a brown precipitate appears. After standing for 3-6 hours, the supernatant is removed to collect the precipitate, dried, and brown or dark brown viscose solid thick yam glycoprotein can be obtained.

Embodiment 2

[0015] Embodiment 2: the preparation of yam glycoprotein

[0016] Take 1000g of the original Chinese yam and crush it into a coarse powder, add 3 times the amount of water to heat, the temperature is 70°C, extract twice, each time for 3 hours, after filtering, combine the two extracts, concentrate 50%, add ethanol with a concentration of 95% Make the alcohol content reach 70% in the concentrated solution, white precipitate appears, let it stand for 3-6 hours, separate the precipitate from the supernatant, and continue to add ethanol with a concentration of 95% to the supernatant to make the alcohol content reach 90% %, a brown viscose precipitate appears, the supernatant is removed, the precipitate is collected, and vacuum-dried to obtain the purer yam glycoprotein in brown or dark brown solid.

Embodiment 3

[0017] Embodiment 3: the preparation of yam glycoprotein

[0018] Take 1000g of yam raw medicine and crush it into large particles, add 5 times the amount of water to heat, the temperature is 100°C, extract 3 times, each time for 2 hours, after filtering, combine the two extracts, concentrate to 50%, and dilute the concentration to 95% ethanol Add it to the concentrated solution to make the alcohol content reach 75%. After the white precipitate appears, extract the supernatant. Continue to add ethanol with a concentration of 98% to the supernatant to make the alcohol content reach 95%. Yellow-brown viscose appears The supernatant was removed, and the precipitate was collected for vacuum freeze-drying to obtain the purer yam glycoprotein in the form of brown crystals.

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PUM

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Abstract

The invention relates to an application of Chinese yarn glycoprotein in improvement of the activities of an oxidative metabolism enzymes of mitochondria of a brain cell. Selection and verification are carried out by an animal test so as to prove that the Chinese yarn glycoprotein can obviously improve the activities of key enzymes namely pyruvate dehydrogenase and alpha-oxoglutarate dehydrogenase used for oxidative metabolism in the mitochondria of the brain cell, so that brain cell deterioration and damage diseases, such as senile dementia, vascular dementia, cerebral infarction and cerebral palsy, accompanying with oxidative metabolism efficiency reduction caused by reduction of the activities of the enzymes can be effectively treated.

Description

technical field [0001] The invention relates to the use of yam glycoprotein in improving the activity of brain cell mitochondrial oxidative metabolic enzymes, in particular to the use of yam glycoprotein in the preparation of drugs for improving the activity of pyruvate dehydrogenase and α-ketoglutarate dehydrogenase in brain cell mitochondria the use of. Background technique [0002] Brain cell mitochondria are one of the main organelles for brain cells to maintain normal physiological activities, and they are also the switch to control brain cell apoptosis. Under normal conditions, the main oxidative metabolic enzymes in mitochondria, pyruvate dehydrogenase and Hydrogenase, oxidizes and metabolizes energy-producing substances to produce energy (ATP) for cells to maintain normal physiological activities. Oxygen, so that brain cells can not produce enough ATP to maintain normal physiological functions, it will produce a series of pathological changes, such as Alzheimer's di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P25/28
CPCA61K38/011A61K36/8945A61K2236/00A61P25/28A61P39/06C07K1/145C07K14/415
Inventor 多力坤·马木特
Owner 新乡博凯生物技术有限公司
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