Spectrophotometry for testing activity of pyruvic acid dehydrogenase system

A technology of pyruvate dehydrogenase and activity, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral characteristic measurement, etc., and can solve problems such as environmental pollution, no spectrophotometry, unstable radioactive substances, etc. Achieve the effect of high sensitivity, small standard error and low price
CN101017134AInactive Publication Date: 2007-08-15HUAZHONG NORMAL UNIV
0 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG NORMAL UNIV
Publication Date
2007-08-15
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

This invention relates to one splitting photometry to test pyruvate dehydrogenase enzyme activity, which comprises the following steps: using splitting meter under situation of underlay of sodium ketopropionate and aid factor magnesium chloride and cocarboxylase using phenazine methyl ester sulfate as electron deliver part and thiazole blue as electron receiver restored by the product of pyruvate dehydrogenase through hydroxyethylation-cocarboxylase with maximum absorptive peak from length for 400 to 430 nanometer into 540 to 640 nanometer; through testing wave length for 540 to 640 nanometer absorptive add volume to determine the restore volume and defining enzyme activity.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The invention relates to a method for measuring the activity of pyruvate dehydrogenase system. Specifically, based on the use of phenazine dimethyl sulfate as the electron carrier, thiazole blue as the electron acceptor, thiazolium blue is reduced by the catalytic product of pyruvate dehydrogenase (E1), and the reduction amount of thiazolium blue is determined to determine pyruvate. Spectrophotometric method of dehydrogenase system activity. Background technique

[0002] The determination of pyruvate dehydrogenase activity is essential in the properties, gene cloning, separation, purification, identification and related research of pyruvate dehydrogenase system. Herbicides and fungicides targeting the ketoacid dehydrogenase system are also of great significance for the study of diseases related to abnormal metabolism of pyruvate. The invention studies the method for measuring activity of pyruvate dehydrogenase system, and establishes an economical a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More