The application discloses a kind of
myoglobin preparation process for
chemiluminescence immunoassay quality control product, it is related to
myoglobin preparation technical field, comprising the following steps:
raw material pretreatment: after removing impurities of fresh
pig heart muscle tissue, it is placed in automatic cleaning
cutting equipment and is
cut and cleaned,
myoglobin extraction:
muscle tissue after pretreatment is placed in
grinding mechanism, and extraction fluid is added to
grind,
centrifugal separation: after extraction, mixed liquid is placed in high-speed
centrifugal separation equipment and is centrifuged, to obtain supernatant, purification treatment: supernatant is purified by multistage
filtration separation equipment, stabilization treatment: after purification, myoglobin solution is added with stabilizer, placed in gradient
temperature control stirring
system and stirred and mixed, low-temperature preservation, solve the problem that
muscle tissue is
cut into meat foam by manual shearing mode,
grinding is not enough, so that separation effect is poor, leading to the problem that myoglobin purity is not high and activity is easily affected.