40 results about "Pig heart" patented technology

The invention relates to an SOD complex capsule and a preparation method thereof. The SOD complex capsule contains resveratrol, coenzyme Q10, goose liver extracts, wheat germ extracts, safflower extracts and tartary buckwheat extracts; the weight ratio of the components is 10-50 : 10-75 : 75-150 : 75-150 : 75-150 : 200-500; every 100g of the complex capsule contains 40000-4000000 activity unit of SOD which is a tadpole extract. The preparation method is as follows: processing grape skins, pig hearts, goose livers, wheat, safflower, tartary buckwheat and tadpole by the steps of breaking, concentration, centrifugal separation, purification and the like to obtain active ingredients of various raw materials, embedding the tadpole extract for several times, mixing the tadpole extract with other components and palletizing the mixture, thus obtaining the finished product. The invention has the function of oxidation resistance, can alleviate physical fatigue and strengthen immunity of human body.

The invention relates to a process for extracting and purifying protease, in particular to a method for extracting and purifying metmyoglobin reductase from cardiac muscle. The method comprises the following steps: obtaining crude extractive liquid from cardiac muscle of an animal, salting out, and separating and purifying metmyoglobin reductase from pig heart by chromatography adopting DEAE-Fast Flow and CM-Sephadex FF ion exchange medium and blue sepharose FF medium. The method has the advantages of high product purity, simple process, low cost, etc., and can fill the blank in bio-reagent preparation and achieve higher economic value.

The invention discloses an FTO gene for intramuscular fat deposition. The FTO gene is an FTO (fat mass and obesity associated) gene which is separated and cloned from Banna miniature pig muscle tissue and is associated with intramuscular fat deposition. The whole length of the CDA (Complete Coding Sequence) of the gene is 1518bp and the CDS nucleotide sequence is shown as SEQ.ID.NO.1. In addition, the CDS nucleotide sequence codes 505 amino acids of which the amino acid sequence is shown as SEQ.ID.NO.2. Through the detection of a tissue expression profile of the FTO gene in seven tissues, such as pig heart, liver, spleen, lung, kidney, muscle and fat, the result shows that the FTO gene is greatly expressed in the fat tissue and the functions of the FTO gene for regulating and controlling the fat deposition in fat cells are preliminarily shown.

A heart valve prosthesis and a method of fabricating the same. The heart valve prosthesis uses a leaflet of pig pericardium (10) or a leaflet of cattle pericardium, which has a suitable thickness according to the size of the valve, and a stent. The heart valve prosthesis is fabricated by placing a valve body over a Dacron body (20), binding the valve body to the Dacron body using stitching fiber (30), rolling the Dacron body into a cylindrical form such that the valve body is located inside, binding opposite edges of the Dacron body to each other using stitching fiber, and inserting the resultant structure into a stent (60), which is knitted using a shape memory alloy wire. The heart valve prosthesis is friendly to and is not rejected by human tissue, is free from deformation, and can be implanted using a non-invasive manner without incising the chest wall of a patient.

The invention discloses a nerve-calming and sleep-helping food, which comprises the following raw materials in parts by weight: 20-30 parts of pig hearts, 2-4 parts of medlar, 1-2 parts of onion stalks, 1-2 parts of fermented soya beans, 4-6 parts of wheat, 1-2 parts of lotus seed, 2-3 parts of jujubes, 2-3 parts of wild jujubes, 2-4 parts of lily, 2-4 parts of euphoria longan and 8-12 parts of rhizoma dioscoreae. The food has the effects of relieving palpitation, calming nerves, supplementing vital energy and reducing blood pressure, is used for treating symptoms such as nerve headache, headache caused by high and low blood pressure, migraine, insomnia, palpitation and the like, and can improve blood circulation of a human body and promote blood supply of brain and cardiac muscle.

The invention relates to a cardiotonic and tranquilizing health care soup, which comprises the raw materials of: 15g of codonopsis pilosula, 10g of finger citron, 1 pig heart, 100g of vegetable hearts, 30ml of vegetable oil, 5g of ginger, 3g of salt, 500ml of chicken broth, 2g of monosodium glutamate, 1g of pepper powder, 10ml of cooking wine, and 10g of scallions. Integrating the efficacy of the codonopsis pilosula and the pig heart, the soup provided in the invention has the functions of qi tonifying, perspiration stopping, and tranquilizing. Being suitable for hyperhidrosis, palpitation, qi deficiency and insomnia, the health care soup mainly treats heart deficiency, insomnia, palpitation, spontaneous perspiration, spirit trance and other symptoms.

The invention provides a method for extracting myoglobin in pig hearts and a human total antioxidant status kit containing the myoglobin. The method comprises the following steps: a buffer is added into fresh pig hearts, homogenate and centrifugation are carried out, ammonium sulfate is added into a crude extract, stirring, precipitation, and centrifugation are carried out, and a supernatant is reserved; ammonium sulfate is added into the supernatant till a saturation state, precipitation is carried out for more than 2 hours, centrifugation is carried out, and the supernatant is removed; an ammonium sulfate containing buffer is used for redissolving the precipitate to an original volume, and filtering is carried out for waiting sampling; a hydrophobic filling material is filled into a column for sampling, balance is carried out till proteins do not flow out, the ammonium sulfate containing buffer is used for washing out, and target protein is collected according to an eluting peak. The method has high output and low extraction cost, obtained myoglobin has good stability and can be applied to a TAS kit, in order to detect concentration of total antioxidant substances in serum, urine and other components in body; at the same time the raw materials have abundant sources and are easy to obtain, and batch production requirements of the kit are satisfied.

The invention discloses a traditional Chinese medicinal preparation for treating mental disease and a preparation method thereof. Each medicament of the Chinese medicinal preparation consists of 6g of acorus root, 6g of tuber curcumae, 5.25g of dahurian angelica root, 3.75g of arisaema root, 3.75g of cinnabar, 6.75g of gansui root, 3.75g of amber, 0.33g of musk and one pig heart. The Chinese medicinal preparation is prepared by a processing method and has the advantages of special effect, quick response, short treatment period, no toxic or side effect and no sequela.

The invention discloses a pig heart processing method and aims at providing a pig heart which is suitable for patients with coronary artery disease to eat. According to the technical scheme of the invention, the pig heart is processed according to the procedures of cleaning, marinating, cutting, roasting and packaging. The pig heart processing method comprises the steps of: (1) cleaning the pig heart, and removing bloodiness in blood vessels of the pig heart; (2) putting the pig heart into an earthware pot, adding water, Chinese angelica, rhizoma ligustici chuanxiong, ginger, star anise and salts into the earthware pot, and heating and marinating the pig heart until the pig heart is savoury; (3) cutting the marinated pig heart into small pieces; (4) roasting the small pieces of the pig heart; and (5) vacuum-packaging the small pieces of the pig heart. According to the technical scheme of the invention, the operation is simple, and the pig heart is suitable for the patients with the coronary artery disease to eat.

The invention relates to a drug mainly for treating cardiovascular disease, which is characterized by comprising raw materials in the following weight portion: 9 to 11 portions of pig heart, 0.4 to 0.6 portion of cinnabar, and 0.2 too 0.4 portion of shelled cedar seed. The drug has the advantages of good curative effect, quick effect, suitability for both acute and chronic cardiovascular diseases, simple raw materials which are easy to obtain, conveniences for preparation and eating, small toxic side effect, and convenient popularization.

The invention discloses a preparation method of a novel absorbable biological membrane. The novel absorbable biological membrane is composed of a three-layer structure which is divided into a pig pericardium layer, a growth promoting layer and a gel layer. The preparation method comprises the following steps that the pig pericardium layer is prepared, most cells of the porcine pericardium layer are removed by using 0.1%-0.5% trypsin, and terminal peptide is removed; then cleaning is carried out for 6 times by using purified water; oscillating is carried out for 1-4 hours by using 0.1%-1% SDS at room temperature at 80-180rpm, and cleaning is carried out for 10 times by using purified water; and the growth promoting layer is prepared, specifically, a bioactive factor solution is added into the rough surface of the decellularized porcine pericardium layer, incubating is carried out at room temperature for 2-4 hours, and then the incubated porcine pericardium layer is freeze-dried. The novel absorbable biological membrane has enough mechanical properties to support the pressure of soft tissues and prevent epithelial cells, fiber cells and the like from invading a bone defect area, and has proper thickness and porosity, so that the membrane has good fitness and comfort; and the novel absorbable biological membrane is beneficial to diffusion of oxygen, nutrient substances and bioactive substances to the tissues and promotes cell growth.

The invention provides a drug for treating pregnant women with a heart disease. The drug is prepared from the following raw materials in parts by weight: 5 to 10 parts of raw haw, 2 to 4 parts of honey, 5 to 10 parts of pig heart, 2 to 4 parts of soybean, 2 to 4 parts of dried longan, 1 to 2 parts of egg, 3 to 5 parts of jujube, 1 to 2 parts of white sugar, 2 to 4 parts of cucumber steam, and 3 to 5 parts of lotus seed. The drug has the advantages of low price and good curative effect, and does not have any side or toxic effect.

The invention discloses a healthy porridge conducive to people with diabetes. The healthy porridge conducive to people with diabetes is prepared from a first component, a second component and a third component. The first component comprises the following raw materials in parts by weight: 2-6 parts of chicken's gizzard-membrane, 4-8 parts of schisandra chinensis, 3-6 parts of actinidia polygama, 4-8 parts of radices paeoniae alba, 2-5 parts of prepared rheum officinale, 7-9 parts of fruit of Chinese wolfberry, 4-9 parts of root of kudzu vine, 3-6 parts of sweet potato leaves, 2-4 parts of watermelon peel, 5-9 parts of dodder weed, 4-10 parts of fresh root of spinach and 10-20 parts of fresh onion. The second component comprises the following raw materials in parts by weight: 60-80 parts of sticky rice. The third component comprises the following raw materials in parts by weight: 18-24 parts of pig pancreas and 16-20 parts of pig heart. The healthy porridge is characterized by the functions of Yin nourishing, heat clearing, liver soothing, vital energy flow regulating, heat and dampness eliminating, kidney and spleen tonifying, and Qi and Yin tonifying and obvious curative effect to diabetes of various types.

The invention provides an instant salt-baked pig heart and a preparation method thereof. A pig heart is pretreated, then, sliced, pickled, salted, air-dried, salt-baked and subjected to inner packing, disinfection and outer packing, and the instant salt-baked pig heart is obtained. The instant salt-baked pig heart doesn't contain any chemical additives, has good quality, can be stored for a long time and spreads fragrance after being lightly chewed in a mouth, the fat, the calories and the cholesterol content are low, nutrition is not lost, the nutrition is richer, and the instant salt-baked pig heart has the feature of salt-baked food and good flavor, is hygienic, has a health-care function, can be eaten at any time and is convenient to carry. The preparation method is high in production efficiency, and large-scale industrial production can be performed.

The invention discloses a marinated pig heart and a production method thereof. The marinated pig heart is prepared from the following raw materials: a fresh pig heart, dry ginger slices, a sweet fermented flour sauce, green onions, orange peel, red cluster pepper, vegetable oil, fruit wine, bone soup, fat incense soybean sauce, a proper amount of refined salt, rock candy, monosodium glutamate, a proper amount of water and seasonings. The production method comprises the following steps: (1) preparing a standby pig heart; (2) preparing a seasoning pack for later use; (3) preparing a soup base for later use; and (4) adding the standby pig heart and the seasoning pack into the soup base, boiling with a strong fire, and slowly cooking with soft fire, so as to obtain the marinated pig heart. The marinated pig heart prepared by virtue of the preparation method has tangy fragrance, rich flavor and special taste, and multiple nourishing raw materials are added in a proper proportion, so that the nutrition value is rich, and the marinated pig heart has certain dietary therapy and health effect; and the process is simple, and the technique is easily grasped, so that the production efficiency is greatly improved, and the production method is suitable for popularization and application.

The invention discloses an intramuscular fat deposition related adiponectin gene, which is obtained from Banna minipig muscular tissues by separating and cloning. The full length of a CDS (coding sequence) of the gene is 732dp, a nucleotide CDS of the gene is shown in SEQ. ID. NO.1, 243 amino acids are coded in the nucleotide CDS, and amino acid sequence is shown in SEQ. ID. NO.2. Results of detection of the adiponectin gene in pig heart, livers, spleen, lungs, kidneys, muscle and fat show that the adiponectin gene is highest in expression quantity in fat tissues, and the function of regulating fat deposition in fat cells is preliminarily indicated for the adiponectin gene.

The invention relates to a method for preparing eight-treasure fragrant meat. The method comprises the following steps: adding eight pig by-products including pig ears, pig bag, preserved pig head meat, pig intestines, pig liver, pig tongue, pig tail and pig heart into hot water which comprises eight natural spices including cumin, cinnamon, Chinese prickly ash, nutmeg, dahurian angelica roots, villous amomum fruits, star anise and myrcia as well as auxiliary materials of cooking wine, table salt, crystal sugar and monosodium glutamate for white pot stewing; slicing and cutting the pig by-products cooked by the white pot stewing into sections; and adding chop soup, chili oil, sour pepper juice, chili oil chilies and Sichuan pepper powder into a stirrer with full stirring and mixing so as to obtain the eight-treasure fragrant meat. The preparation method of the eight-treasure fragrant meat disclosed by the invention processes eight by-products of slaughtered pigs into the eight-treasurefragrant meat food which has the advantages of nutrition, health, unique flavor, color with color, taste with taste and integrates seven tastes of sourness, sweetness, spiciness, saltiness, freshnessand fragrance into one. The method has important significance in improving the comprehensive utilization and economic benefit of pig by-products, meeting the needs of good life for people, and increasing new varieties of meat foods.

The invention discloses a method for detecting sedative drug metabolism residues in animal tissues, which adopts a dispersive solid-phase extraction-high performance liquid chromatography triple quadrupole mass spectrometry method to simultaneously determine chlorpromazine, promethazine, methylthiazine and metabolites thereof such as chlorpromazine sulfoxide, 2-chlorophenothiazine, promethazine sulfoxide, 2, 6-dimethylaniline in pork, pork liver, pig kidney, pig heart and pig lung. The method optimizes the detection conditions, can accurately detect the sedative drugs in animal tissues, has accurate and reliable detection results, provides reliable data for the measurement of sedative drug metabolic residues, has significant economic benefits, and has wide market prospects. The method has a good reference value for guiding the measurement of drug residues.

The invention discloses an intramuscular fat deposition SCD (Stearoyl-CoAdesaturase) gene which is separated and coned from Banna minipig muscular tissues and related to intramuscular fat deposition; the overall length of a CDS (Coding Sequence) of the gene is 1080bp; and a CDS nucleotide sequence of the gene is SEQ.ID.NO.1. The CDS nucleotide sequence codes 359 amino acids and an amino acid sequence is SEQ.ID.NO.2. Tissue expression spectrums of the SCD gene in seven tissues such as pig heart, liver, spleen, lung, kidney, muscle and fat are detected; and the result shows that the expression value of the SCD gene in the fat tissue is the highest and the fat deposition control function of the SCD gene in fat tissues is preliminarily indicated.

The invention relates to seasonings, in particular to special asparagus seasoning for cooking of pig hearts and mainly solves the problems that known asparagus has the advantages of being fragrant, delicious, high in edible value and the like, but the eating period is short and cooked pig hearts with the asparagus as the seasoning are unavailable in other seasons. The seasoning with different flavors is prepared from ingredients as follows: 50-80 g of asparagus powder, 5-10 g of black tea powder, 5-10 g of radix scutellariae powder, 3-5 g of boat-fruited scaphium seed powder, 3-5 g of ginger powder, 3-5 g of garlic powder, 3-5 g of lotus seed powder and 6-10 g of fragrant or spicy auxiliary materials. The production cost is low, equipment is simple and raw materials are rich. The seasoninghas functions of enhancing umami, promoting appetite and strengthening the body and can be stored as long as 12 months or longer.

The invention discloses a drug for treating a coronary heart disease and a preparation method thereof. The drug is a medicament prepared from 25-35 parts of zollinger gromwell fruits, 5-15 parts of panax notoginseng and 20-30 parts of inula flowers. The preparation method of the drug comprises the steps that the medicinal materials and 350-450 parts of pork hearts or pork ribs are put into a pot and then stewed for 45-60 min by adding clear water, and then the drug is obtained. The drug can significantly relieve coronary heart disease angina of a patient and improve the physical signs of the patient, no side effect exists, and the effect is quickly achieved; the adopted preparation method is simple and easy to operate; the drug is clinically used by 32 cases, and the total effective rate is 100 percent.

The invention provides a production technology of stewed pig hearts, and relates to the field of food processing. The production technology comprises the following steps of (1) preparing brine: addingspicy materials to a soup stock, performing decoction for 4-6 hours, performing filtration, and taking the obtained filtrate; (2) preparing thick marinade: adding polycrystalline crystal sugar to thebrine, and performing concentration; (3) pretreating raw materials: unfreezing frozen defatted pig hearts, washing away bloodstains, removing oil sinews, putting treated pig hearts into cold water, performing cooking, and fishing out the cooked pig hearts; (4) performing cooking: putting the pig hearts into the brine, performing cooking, and adding seasoning; (5) performing cooling: cooling the seasoned pig hearts to room temperature; (6) performing coating: uniformly coating the pig hearts with the thick marinade; and (7) performing packaging: sterilizing the pig hearts, adding a food preservative and a fresh keeping agent, performing vacuum packaging, and enabling finished products to be dispatched from the factory. According to the production technology disclosed by the invention, rawmaterials are carefully selected, produced finished products are uniform and golden in color, rich and long in fragrance, fresh and fragrant in taste, soft in mouth feel, pure in taste, unique in taste, rich in flavor, simple in technology, short in production cycle and high in production efficiency, and mass production is facilitated.

The invention relates to a heart acellular matrix composite temperature-sensitive gel, which is characterized in that the composite temperature-sensitive gel is composed of a chitosan temperature-sensitive gel and pig heart acellular matrix particles (the particle size is 10-100 [mu] m). The invention also provides a preparation method of the composite temperature-sensitive gel, which comprises the following steps: (1) preparing a chitosan solution, and dropwise adding a beta-sodium glycerophosphate solution into the chitosan solution under ice bath and stirring conditions to prepare a low-temperature chitosan temperature-sensitive gel gelling solution; (2) preparing pig heart acellular matrix particles, wherein the particle size is 10-100 [mu] m; (3) adding pig heart acellular matrix particles into the low-temperature chitosan temperature-sensitive gel forming solution, wherein the particle concentration is 0.2-6% (w / v, g / ml), and thus obtaining a heart acellular matrix composite temperature-sensitive gel forming solution; and (4) quickly converting the low-temperature cardiac acellular matrix composite temperature-sensitive gel forming solution into a gel state under the condition of 33-37 DEG C. The gel-forming solution of the heart acellular matrix composite temperature-sensitive gel can reach the damaged part of the heart through injection, composite gel is rapidly formed in situ, and the effect of repairing the damaged cardiac muscle tissue is achieved.

The invention discloses a corn and mushroom cookie with cholagogue and diuretic effects. The cookie is made of ingredients including 280-290 parts by weight of flour, 90-100 parts by weight of fresh mushrooms, 25-30 parts by weight of pig hearts, 70-80 parts by weight of corn flour, 35-40 parts by weight of bean seedlings, 50-60 parts by weight of red date vinegar, 2-3.2 parts by weight of Agastache rugosus leaves, 3-4 parts by weight of baking powder, 85-90 parts by weight of white granulated sugar, 3-4 parts by weight of salt and appropriate parts by weight of water. The mushroom cookie disclosed by the invention is made of the abundant ingredients and is characterized in that multiple auxiliary ingredients such as the pig hearts, the corn flour and the red date vinegar are added, and the finished product cookie has better nutritious values because of rational matching and processing; in addition, the main ingredient mushrooms are gradually and uniformly dried during the processing, so that internal humidity is kept consistent with superficial humidity; certain stability is maintained; a special flavor is maintained; corn, red date and vinegar tastes can be tasted during eating; and long-term eating of the cookie is beneficial for physical health.

The invention discloses a health medicated food suitable for diabetic patients. The health medicated food is prepared from a first component, a second component and a third component, wherein the first component is prepared from the following raw materials: semen arecae, endothelium corneum gigeriae galli, silvervine, pilea cavaleriei, wolfberry fruit, astragalus membranaceus, schisandra chinensis, longflower stringbush root, white paeony root, mantis egg-case, lalang grass rhizome, puerarin and rhizoma alismatis; the second component comprises pearl barley and millet; and the third component comprises a pig bag and a pig heart. According to the health medicated food prepared by the method, the target of stably and rapidly lowering blood glucose is reached by improving the immune functions of preventing and resisting diseases of a human body, regulating internal secretion, improving microcirculation and regulating and repairing insulin; and the health medicated food is mild in medication and scientific in proportion, and has the effects of promoting the production of body fluid to relieve thirst, reinforcing the stomach, tonifying the spleen, nourishing yin, and reinforcing the kidneys.

The invention discloses a medicine for treating fatty liver, prostatitis, diabetes, pneumonia and migraine. The medicine is composed of 19 Chinese herbal medicines including inonotuso bliquus, Huidouba, rhodiola rosea and the like; the medicine pickled together with poultry eggs can effectively treat fatty liver, prostatitis and diabetes; medicine juice obtained by pickling the medicine with the poultry eggs is combined with lamb lung, pig lung and cattle lung to effectively treat pneumonia and diabetes; the medicine juice obtained by pickling the medicine is combined with lamb tripe, pork tripe and beef tripe to effectively treat diabetes; the medicine juice obtained by pickling the medicine is combined with sheep brain, pig brain and cattle brain to effectively treat migraine and diabetes; the medicine juice obtained by pickling the medicine is combined with lamb liver, cattle liver and pig liver to effectively treat diabetes; the medicine juice obtained by pickling the medicine is combined with lamb heart, cattle heart and pig heart to effectively treat diabetes. According to the technology of the invention, animal innards are mixed with table salt and medicine juice pickled with duck eggs and chicken eggs to obtain a compound medicine. The medicine can be taken as vegetables at meal to reduce economic burden of patients, and is a green health product.

The invention discloses a herbal cuisine for inhibiting gastrointestinal reaction of chemotherapy. The herbal cuisine is mainly prepared from the following raw medicine materials in parts by weight: 9-15 parts of astragalus membranaceus, 12-20 parts of fructus amomi, 9-15 parts of Chinese angelica, 10-16 parts of kudzuvine root, 8-16 parts of dried tangerine or orange peel, 8-12 parts of all grass of anderson cirrhopetalum, 6-15 parts of bighead atractylodes rhizome, 15-20 parts of pig heart, 9-12 parts of salvia chinensis, 6-10 parts of hedyotis diffusa, 10-15 parts of radix glycyrrhizae, 20-35 parts of dog kidney, 6-10 parts of morinda officinalis and 40-60 parts of pigeons. The herbal cuisine is prepared by selecting animal and plant materials, ensures safety, has no toxic or side effect, has the efficacies of tonifying kidney, tonifying qi, regulating qi, strengthening spleen, raising yang, stopping diarrhea and the like, has good inhibition effect on gastrointestinal reaction of chemotherapy, and not only simply stops emesis and diarrhea, but also improves the immunity of the organism.

The invention relates to a two star-grade dish with effects of intelligence increasing, physical strength increasing, wisdom increasing, wealth increasing and life increasing, wherein nonglutinous rice, long-shaped rice, millet, sorghum, millet and other main food are intelligently cooked with high quality mountain spring water, scatter-feed pork, portulaca oleracea l, donggan, brassica campestris, brassica pekinensis, orchid, lactuca sativa, radish, and other main raw materials are intelligently cooked with sauce, vinegar, allium fistulosum l and garlic, and the skin of soybean milk, pig head meat, pig neck meat, pig marrow, pig heart, pig liver, pig lung, pig tripe, pig intestine, hog bladder, pig tongue, pig trotter, pig tail, bamboo shoot clothes, purple sweet potato vermicelli, fresh bean curd thin sheets, small blocks of hot pepper, fresh celery, white tremella, fresh eleocharis tuberosa, soybean sprouts, mung bean sprouts, peanut buds, Chinese wolfberry buds, kelp, corn particles, small blocks of carrot, white radish strips, sesame oil, peanut oil, colza oil, tofu residue, white tremella, lotus root, black fungus, sweet potato vermicelli, radix puerariae vermicelli, Chinese wolfberry, porphyra, tofu, tomato, flammulina velutipes, dried bamboo shoot, corn powder, barley powder, oat powder, wheat powder, tuckahoe powder, flax powder, and chestnut powder are intelligently cooked to develop the intelligence and life balls, the intelligence and life soup, the intelligence and life tea, the intelligence and life nuts, and the intelligence and life fruit. With the technical scheme of the present invention, the significant effects of intelligence increasing, physical strength increasing, wisdom increasing, wealth increasing and life increasing can be developed.