8 results about "Pig heart" patented technology

The invention relates to the technical field of active peptides, in particular to a myocardial active peptide capable of relieving heart failure and protecting heart and a preparation method thereof. The method takes pig hearts as raw materials, and sequentially comprises the following steps: pretreating the pig hearts, adding water, and homogenizing; adding alkaline protease to carry out first constant-temperature enzymolysis; adding neutral protease and flavourzyme to carry out second constant-temperature enzymolysis; then lipase is added for third-time constant-temperature enzymolysis, and enzyme deactivation and cooling are carried out; and finally, adding activated carbon into the enzymatic hydrolysate, filtering with diatomite, and spray-drying to obtain the myocardial active peptide. According to the method, the yield and the purity of the active peptide are remarkably improved through a synergistic process of three-step enzymolysis and activated carbon purification, the obtained product is rich in small molecule peptide with a definite heart protection function, and a new way is provided for high-value utilization of pig heart resources and development of functional products for preventing and treating heart failure.

The application provides a live pig heart rate detection method and system based on FMCW radar and deep learning, relates to the field of intelligent livestock breeding, and comprises signal synchronous acquisition, data preprocessing and sample construction, spatial micro-motion feature extraction, frequency domain enhancement and interference suppression, long-time sequence dependence modeling, heart rate regression output, model training and online deployment. The method makes up for the defects of the existing non-contact live pig heart rate detection technology in the robustness under a complex breeding environment, realizes continuous, automatic and low-interference monitoring of the live pig heart rate, and is convenient for breeding managers, veterinarians and experimental personnel to master the vital sign changes of live pig individuals in real time.

The application discloses an artificial blood vessel stent with anticoagulation performance and a construction method thereof. After a pig heart aorta blood vessel is subjected to decellularization treatment, a pre-gel solution is prepared by compounding polyvinyl alcohol and methacrylated gelatin, a designed model is printed into a hollow tubular mold by using 3D printing, the pre-gel solution is injected into the mold to obtain an artificial blood vessel stent with controllable shape and size after cross-linking, and finally, the artificial blood vessel stent loaded with heparin is obtained by loading the heparin on the prepared stent. The material for preparing the pre-gel solution is selected, and the advantages of the material functions can be complemented. The decellularized matrix is beneficial to cell adhesion, proliferation and differentiation; the compounding of polyvinyl alcohol and methacrylated gelatin improves the mechanical properties of the stent and improves the cell adhesion. The heparin is loaded on the artificial blood vessel stent to improve the anticoagulation performance of the stent and prevent the formation of thrombus. The stent serves as an excellent biomedical material and has a wide application prospect in the fields of cardiovascular tissue engineering and biology.

The application discloses a kind of myoglobin preparation process for chemiluminescence immunoassay quality control product, it is related to myoglobin preparation technical field, comprising the following steps: raw material pretreatment: after removing impurities of fresh pig heart muscle tissue, it is placed in automatic cleaning cutting equipment and is cut and cleaned, myoglobin extraction: muscle tissue after pretreatment is placed in grinding mechanism, and extraction fluid is added to grind, centrifugal separation: after extraction, mixed liquid is placed in high-speed centrifugal separation equipment and is centrifuged, to obtain supernatant, purification treatment: supernatant is purified by multistage filtration separation equipment, stabilization treatment: after purification, myoglobin solution is added with stabilizer, placed in gradient temperature control stirring system and stirred and mixed, low-temperature preservation, solve the problem that muscle tissue is cut into meat foam by manual shearing mode, grinding is not enough, so that separation effect is poor, leading to the problem that myoglobin purity is not high and activity is easily affected.

The application provides a live pig heart rate detection method and system based on FMCW radar and deep learning, relates to the field of intelligent livestock breeding, and comprises signal synchronous acquisition, data preprocessing and sample construction, spatial micro-motion feature extraction, frequency domain enhancement and interference suppression, long-time sequence dependence modeling, heart rate regression output, model training and online deployment. The method makes up for the defects of the existing non-contact live pig heart rate detection technology in the robustness under a complex breeding environment, realizes continuous, automatic and low-interference monitoring of the live pig heart rate, and is convenient for breeding managers, veterinarians and experimental personnel to master the vital sign changes of live pig individuals in real time.

The invention relates to the technical field of bioengineering, in particular to a preparation method of stable natural alanine aminotransferase, which comprises the following steps: acquiring a pig heart raw material, removing fascia and fat, adding a first buffer solution, and crushing to obtain a first enzyme extracting solution; adding ammonium sulfate, adjusting the pH value by using a second buffer solution, centrifuging, and taking supernate to obtain a second enzyme extracting solution; adding solid ammonium sulfate for salting-out precipitation, centrifuging to take precipitate, and dialyzing with a second buffer solution to obtain a third enzyme extracting solution; purifying by using an ion exchange column, eluting by using a third buffer solution, a fourth buffer solution and a fifth buffer solution, and dialyzing and exchanging by using a sixth buffer solution to obtain a fourth enzyme extracting solution; the alanine aminotransferase prepared by the method is high in activity, good in stability, low in cost and high in process repeatability, and the alanine aminotransferase is high in activity, good in stability and high in process repeatability.

The invention relates to the technical field of bioengineering, in particular to a preparation method of stable natural creatine kinase. Comprising the following steps: removing fascia fat from a pig heart, adding a DK1 buffer solution, and crushing by a meat grinder to obtain an enzyme extracting solution A; adding ammonium sulfate into the crude enzyme extract A for multiple times, adjusting the pH value by using DK2, centrifuging, and taking supernatant to obtain an enzyme extract B; adding solid ammonium sulfate into the enzyme extracting solution B to carry out salting-out precipitation, centrifuging to take precipitate, and dialyzing with DK2 to obtain an enzyme extracting solution C; purifying through an ion exchange column, and sequentially eluting through a DK3 buffer solution to obtain a creatine kinase solution; carrying out concentration dialysis liquid change treatment on creatine kinase by using DK4 to obtain an enzyme extracting solution D; adding a liquid protective agent into the enzyme extracting solution, and carrying out stabilizer research; through the mode, high-activity and high-stability creatine kinase can be obtained, the process repeatability is high, the cost is low, and large-scale production of the process is facilitated.

The application discloses a fish oil composition with the effect of improving cardiovascular health of pet cats. The composition provided by the application comprises the following components: fish oil, B group vitamins, pig heart hydrolyzed powder, taurine, L-carnitine or a salt thereof, vitamin C and vitamin E. Through reasonable compounding of the composition, the experiment proves that the composition has the effect of significantly improving cardiovascular health of pet cats, and does not obviously cause side effects on pet cats.