199results about How to "Active influence" patented technology

The invention discloses medicinal sustained-release hydrogel as well as a preparation method and an application thereof. Monodisperse medicament-containing calcium alginate microspheres are prepared by adopting a high-pressure electrostatic sessile drop method, to realize direct encapsulation of growth factors and keep the activity of medicaments; and the medicament-carrying calcium alginate microspheres are put into the netlike structure of the hydrogel to realize sustained release of the medicaments. The matrix of the hydrogel contains carboxymethyl chitosan and polyvinyl alcohol, the volume ratio of the carboxymethyl chitosan solution to the polyvinyl alcohol solution is n: (6-n), 0<n<=4, and the release rate of the medicaments is controlled through different volume ratios.

The invention discloses an adherent-cultured cell activity detection method and device based on impedance spectroscopy. An interdigital electrode, which is parallel with the bottom surface of a culture dish, is arranged at the bottom of the interior of the culture dish; two poles of the interdigital electrode are connected to a controller by virtue of lead wires; a DDS (direct digital synthesizer) frequency generator, which is controlled by a single chip microcomputer in the controller, is promoted to transmit sine waves which are in n pieces of frequencies from high frequency to low frequency; a current excitation signal, after being transformed by a voltage-controlled current source, is outputted to the interdigital electrode; n pieces of response voltages of the interdigital electrode, by virtue of a differential amplifier, are inputted to a single chip microcomputer after undergoing analog-to-digital conversion; the single chip microcomputer is used for calculating a complex impedance value under different frequencies, and is used for calculating a dual-layer capacitance value in accordance with an equivalent circuit model and the complex impedance value; the capacitance value of a dual-layer capacitor in an equivalent circuit serves an index for assessing cell activity, so that more accurate cell activity information can be obtained; and the method and the device can be used for monitoring the cell activity in real time, cannot damage the steady state of cells and can achieve quantitative measurement on the cell activity.

The invention discloses a high-permeability concrete sealed waterproofing agent and a preparation method thereof. Most of permeable waterproofing agents on the market presently have the problems of too high surface tension, high viscosity, insufficient permeation depth, bad sealing effect for microcracks in concrete, and unobvious improvement for the waterproofing and anti-permeation performance of concrete. The waterproofing agent disclosed by the invention comprises 500-900 parts of lithium silicate solution, 5-50 parts of modifier, 1-20 parts of retarder, 0-50 parts of reinforcing agent, 5-50 parts of permeating agent and 0-200 parts of deionized water, wherein the lithium silicate solution is a colourless and transparent aqueous solution with a modulus of 4-5 and a solid content of 20-30%; and the permeating agent is one or more of polyether-modified organosilicone and fluorocarbon surfactant. According to the waterproofing agent disclosed by the invention, the lithium silicate solution is modified through the modifier, and the high-efficiency permeating agent and retarder are adopted, thus the surface tension of the waterproofing agent is greatly reduced, and the permeation depth is increased.

The invention provides a coal tar hydrogenation system and a coal tar hydrogenation method. The method comprises a hydrogenation refining reactor, a separation unit and a hydrogenation cracking reactor, wherein the hydrogenation refining reactor is an up-flow fixed bed reactor, the hydrogenation cracking reactor is a down-flow fixed bed reactor, and the separation unit is a middle liquid separation tank; gas-liquid separation is performed on raw oil and hydrogen after a hydrogenation refining reaction, a part of liquid-phase heavy fractions is separated, one path is used as circulating oil and returned to perform the hydrogenation refining reaction again, and the other path is used as quenching oil after being cooled and added between catalyst bed layers for the hydrogenation refining reaction to play a role of cooling; and the other part of the liquid-phase heavy fractions is used as refined generated oil and mixed with hydrogen to perform a hydrogenation cracking reaction, and the obtained reaction product is combined with gas-phase light fractions after the gas-liquid separation to form the final product. The system and the method provided by the invention are low in equipment cost, simple to operate, capable of prolonging the life of the catalysts of the hydrogenation refining reactor and the hydrogenation cracking reactor, and suitable for raw oil with worse conditions.

The invention discloses a catalytic combustion catalyst and a preparation method thereof. The catalyst uses honeycomb ceramic as a supporter, and an aluminasol coating and a slurry coating are sequentially arranged on the surface of the honeycomb ceramic supporter; the aluminasol coating is positioned between the ceramic supporter and the slurry coating; Pt, Zr and La metal element active components are supported on the surface of the slurry coating; the Pt/Zr/La mol ratio is (0.1-1):(1.5-4.5):(1.5-4); and every liter of catalyst supports 0.2-3.5g of noble metal Pt active component. The preparation method of the catalytic combustion catalyst comprises the following steps: supporter pretreatment, aluminasol preparation, coating slurry preparation, sol application of supporter, slurry application, and metal active component supporting. The catalytic combustion catalyst has the advantages of long service time, high removal rate and the like in the catalytic combustion process of treating organic waste gas, thereby ensuring the long-term stable operation of the catalytic combustion device.

The present invention is one medium temperature and low pressure process to prepare biological diesel oil with animal and plant oil and grease and waste oil and grease as main material. The preparation process includes the following steps: 1. mixing oil and grease 1000 weight portions, methanol 150-200 weight portions and catalyst 5-30 weight portions inside a reactor, and heating and pressurizing to 150-300 deg.c and 0.15-1.57 MPa to reaction for 4-6 hr; 2. lower to normal pressure, distilling to eliminate residual methanol, and stilling to separate out catalyst and glycerin water in the lower layer and to obtain crude biological diesel oil product; 3. washing with saturated table salt solution containing 10úÑ concentration Na2CO3 to obtain neutral crude biological diesel oil product; and 4. continuous vacuum distilling to collect 130-190 deg.c fraction as the biological diesel oil product at vacuum degree 5 mmHg. The present invention has the advantages of short reaction time, etc.

The invention discloses a pH-responsive magnetic metal organic framework composite nano-material and its preparation method and use. The pH-responsive magnetic metal organic framework composite nano-material comprises Fe3O4 nanoparticles, polymer layers coating the surfaces of the Fe3O4 nanoparticles and a metal organic framework growing on the polymer layers. The polymer layer comprises a first polymer chelated with metal ions or comprises the first polymer chelated with metal ions and a hydrophilic second polymer. The metal organic framework is formed from Fe<3+> and a phenylboronic acid derivative containing at least one carboxyl group or at least two boronic acid groups through a coordination bond. The composite nano-material provided by the invention has good magnetic response performances and can realize reversible and highly selective capture and release of glycoprotein in different pH environments, and the pH environment is relatively mild so that the influence on the glycoprotein activity is avoided.

A new method for controlling membrane fouling in a membrane-bioreactor relates to a technology for controlling fouling of a membrane component in a membrane-bioreactor, and is suitable for treating water and waste water by a membrane-bioreactor. The invention forms a dual system of biofilm method and membrane-bioreactor by adding a certain amount of filler in the reactor as the carrier of the biofilm, thereby reducing the pollution rate of the surface of the membrane module; at the same time, the optimized aeration method can By controlling the metabolism of microorganisms to further slow down membrane fouling and reduce energy consumption. The method of the present invention does not require structural modification of the original membrane-bioreactor, only needs to add a certain amount of filler, and the method of the present invention can further reduce energy consumption, and is suitable for membrane-bioreactions in the form of various membrane modules It has strong practicability, wide application range, low operating cost, and convenient operation and management. The invention integrates the biofilm method and the membrane-bioreactor efficiently, and has an optimized aeration mode, can well control membrane pollution, reduce energy consumption, and realize long-term stable operation of the membrane-bioreactor.

The invention relates to a resistance-type gas sensor with two support suspension beams and a four-layer structure and a manufacturing method for the sensor. The sensor comprises a substrate frame, a heat insulation cavity, a heating membrane area, a transition area, the support suspension beams, a heating resistance wire, a power supply lead, a power supply electrode, interdigital electrodes, a detection lead, a detection electrode and a sensitive membrane. The resistance-type gas sensor is characterized in that: the heating membrane area positioned above the heat insulation cavity is connected with the substrate frame by the transition area and the support suspension beams; the heating resistance wire is arranged on the heating membrane area in a mode of a crease line and is connected with the power supply electrode on the substrate frame through the power supply lead; the interdigital electrodes are arranged in a gap of the heating resistance wire and are connected with the detection electrode by the detection lead; and the sensitive membrane is positioned on the heating membrane area to cover the integral heating resistance wire and the interdigital electrodes, and is electrically connected with the interdigital electrodes.

The invention relates to an SOD complex capsule and a preparation method thereof. The SOD complex capsule contains resveratrol, coenzyme Q10, goose liver extracts, wheat germ extracts, safflower extracts and tartary buckwheat extracts; the weight ratio of the components is 10-50 : 10-75 : 75-150 : 75-150 : 75-150 : 200-500; every 100g of the complex capsule contains 40000-4000000 activity unit of SOD which is a tadpole extract. The preparation method is as follows: processing grape skins, pig hearts, goose livers, wheat, safflower, tartary buckwheat and tadpole by the steps of breaking, concentration, centrifugal separation, purification and the like to obtain active ingredients of various raw materials, embedding the tadpole extract for several times, mixing the tadpole extract with other components and palletizing the mixture, thus obtaining the finished product. The invention has the function of oxidation resistance, can alleviate physical fatigue and strengthen immunity of human body.

The invention belongs to the technical field of microbial immobilization and particularly relates to an acidophilic bioleaching microorganism inclusion and a preparation method thereof. The acidophilic bioleaching microorganism inclusion adopts a net stricture formed through crosslinking on the surface layer of a polyurethane porous hydrophilic filler carrier and is prepared from polyurethane porous hydrophilic filler, an inclusion agent, carbonate, an acidophilic bioleaching microorganism suspension, a crosslinking agent and a stabilizer, wherein coronate is one or more of sodium carbonate, sodium bicarbonate, potassium carbonate and potassium bicarbonate; the stabilizer is a microorganism culture solution containing polyvalent metal ions; the pH of the acidophilic bioleaching microorganism suspension is smaller than 3.0. The preparation method of the inclusion comprises step of sequentially immersing the hydrophilic filler expanding with water into the suspension containing an inclusion solution and carbonate, the microorganism suspension, a crosslinking solution and a stabilization solution. The problems that conventional inclusions have poor vapor permeability and continuous production of microorganism solutions with high activity and high cell concentration in the culture process cannot be considered are solved.

The invention discloses a kit for detecting protein glycosylation subtype and a detection method thereof. The detection kit comprises agglutinin, confining liquid, a biotin labeled antibody, horse radish peroxidase labeled streptavidin, a 3',3',5',5'-tetramethyl benzidine substrate developing solution, a stop solution and a cleaning buffer solution. The detection method comprises the following steps: fixing the agglutinin onto a polystyrene solid phase carrier to be used for recognizing and binding glycoprotein in the sample based on specific recognition of agglutinin and an oligosaccharide chain, recognizing the glycoprotein needing to be detected by virtue of specific binding of the protein and the antibody thereof, and realizing semi-quantitative analysis of the glycoprotein. According to the detection kit and the detection method disclosed by the invention, semi-quantitative detection of single protein glycosylation subtype can be realized, and the kit is easy and convenient to operate and low in cost.

The invention discloses a rotary desulfurization and denitrification reactor and a system. The reactor comprises a columnar shell, a rotary shaft and a clapboard, wherein the rotary shaft is mounted in the columnar shell, the clapboard is fixedly arranged on the rotary shaft to divide the columnar shell into at least two chambers, the chambers are filled with catalysts, an SO2 adsorbent is positioned above the nitrogen oxide catalyst, and the rotary shaft rotates under the driving of a motor; a smoke inlet and a reduction gas outlet are formed at the top of the columnar shell, the smoke inlet is arranged opposite to the reduction gas outlet, a smoke outlet and a reduction gas inlet are formed in the bottom of the columnar shell, the smoke outlet is positioned below the smoke inlet, and the reduction gas inlet is positioned below the reduction gas outlet. The two catalysts are overlapped vertically, and smoke flow from top to bottom, so as to realize simultaneous adsorption of SO2 and nitrogen oxide. due to adoption of the rotary reactor, the reduction gas flows from top to bottom, desorption and reaction of SO2 and nitrogen oxide are realized.

The invention discloses a method for making a stabilized lactic acid bacteria product. The method includes the steps of (1) preparing lactic acid bacterial fermentation liquor, (2) making enveloping materials, (3) carrying out in-situ enveloping, (4) carrying out lactic acid bacterial fermentation liquor separation, and (5) carrying out enveloping. By means of the method, in-situ enveloping is carried out after lactic acid bacteria are fermented, the pressure withstanding capacity and the shearing force withstanding capacity of the lactic acid bacteria are improved, damage, caused by post-processing, to bacteria bodies is reduced, and the activity of the lactic acid bacteria is kept; core particles are firstly made and then coated with the lactic acid bacteria, lactic acid bacteria particles are made, and influences of the high temperature and the pressure in the particle making process on the activity of the bacteria bodies are avoided. The lactic acid bacteria are enveloped through the composite materials, and it can be guaranteed that the bacteria bodies can smoothly pass through the stomach of an animal and are disintegrated and released under the effect of intestinal tract enzymes to play the probiotic role.

The invention discloses a composite electrolyte membrane for oxygen production. An active cathode layer and a cathode support layer are sequentially arranged on one side of an electrolyte layer from inside to outside, and an active anode layer and an anode support layer are sequentially arranged on the other side of the electrolyte layer from inside to outside, wherein the electrolyte layer is 5 to 50 mu m thick; the active cathode layer/active anode layer is 20 to 50 mu m thick; the cathode support layer/anode support layer is 40 to 120 mu m thick. According to the composite electrolyte membrane, a structural composition of the electrolyte membrane is optimized to endow the product with strong strength, low resistance and high reactivity, so that a technical bottleneck in the field of high-temperature oxygen permeating membranes is broken, and application fields of the electrolyte membrane are expanded; in addition, existing technical problems about sealing are effectively solved, and the product has the advantages of high yield and low cost, so that industrial application is facilitated.

The invention relates to a method for preparing a high antibacterial activity Epsilon-poly-L-lysine (Epsilon-PL) component; the high antibacterial activity component Epsilon-PL product is obtained by using micro-filtration, ultrafiltration and nanofiltration combination multi-level eparation technology for obtaining Epsilon-PL with different molecular weight distribution, determining the molecular weight range of the optimum antibacterial activity component by bacteriostasis experiment, and further being collected and dried. By using the method, the high-purity Epsilon-PL with different molecular weight ranges can be obtained; and as a membrane separation technology is adopted, any effect on the activity of Epsilon-PL is not caused, thus being favorable to research of the Epsilon-PL with different molecular weight.

The invention provides a tissue engineering scaffold, a preparation method thereof and a 3D printing ink capable of being directly biologically mineralized. The tissue engineering scaffold comprises ahigh molecular hollow tubular structure, and the surface of the scaffold is uniformly covered with a nano-hydroxyapatite layer with controllable thickness. The tissue engineering scaffold can be widely applied to filling and reconstruction of bone tissues. The preparation method of the tissue engineering scaffold is carried out by virtue of shell-core coaxial spray head 3D printing under bio-safety conditions, and a three-dimensional scaffold stacked by hollow pipes can be efficiently prepared. The scaffold comprises an open macroporous structure and a controllable hollow pipe structure. Compared with straight-through hole scaffolds prepared by virtue of an existing template method, the tissue engineering scaffold has two types of hole structures, and the tubular orientation is controllable. The uniformly-distributed and thickness-controllable nano-hydroxyapatite layer is rapidly mineralized on the surface of a high molecular hollow tubular scaffold, so that the adhesion and multiplication of cells and the osteogenic differentiation can be remarkably improved.

The solid phosphoric acid catalyst of the present invention has the chemical general expression of A1BaWb(P2O5)c.(H2O)d, where A is Si, B is B, Sb, Bi, Mg or their mixture, W is tungsten, and a, b, c and d are corresponding molar portions when component A is 1. The preparation process of the catalyst includes preparing phosphate polymer with preheated phosphoric acid polymer and component B; mixing phosphate polymer with the mixture of component A and component W; and the forming, drying, roasting and activating of the formed material; with component W being introduced in the form of heteropolyacid of W. The catalyst has high catalytic activity, high water resistance and high crushing resistance, and may be used in industrial production.

The invention provides a preparation method of mesenchymal stem cell fragmentation factor freeze-dried powder with multiple tissue sources. According to the method, mesenchymal stem cells are separated from adipose tissues, mveloid tissues and umbilical cord tissues of a mammal, purified, and subjected to mixed culture, in the mixed culture process, supernate is collected and cultured in every stages, stem cells obtained through mixed culture are subjected to ultrasonication, stem cell fragmentation factors are collected, through two-time freeze-drying concentration treatment, the stem cell fragmentation factor freeze-dried powder is prepared, the whole freeze-drying process is maintained at 20 DEG C or below, heating is conducted step by step slowly, effects of temperature change on the activity of the stem cell factors are prevented, in a low-temperature low-pressure environment, crystal water slowly sublimates, in the presence of a freeze-drying protective additive, other componentsof the stem cell factors are not reduced, the activity is not affected, a compound which is easy to quantify and rich in mesenchymal stem bioactive factors is obtained, and the problem that when stemcell factors are concentrated, cell factor type loss is caused by different molecular weights of the stem cell factors is solved.

The invention relates to a high temperature sintering resistive loading type noble metal catalyst for catalytic oxidation for carbon monoxide and a preparation method thereof. The catalyst comprises acarrier, active components and a cladding shell layer, wherein the carrier is TiO2 or CeO2 nanoparticles; the active components are noble metals Au, Pt or Pd nanoparticles; the active components areloaded on the surface of the carrier; the cladding shell layer which is SiO2 shell layer and clads the surfaces of the carrier nanoparticles and the noble nanoparticles of the active components. The method is simple; the condition is mild; the period is short; massive synthesizing can be carried out; and the environment is influenced a little.

The invention relates to a preparation method of a porous silicon coated zero-valent iron material for in-situ remediation of groundwater pollution, and belongs to the field of nano and new environmental remediation materials. According to the preparation method, ferric chloride is added to a mixed solution of ethyl alcohol, water and tetraethyl orthosilicate, after even stirring is carried out, potassium borohydride or a mixed solution of sodium borohydride and sodium hydroxide is dropped, and after the reaction is completed, magnetic separation, washing and vacuum drying are carried out. Thepreparation process is carried out under the protection of inert gas. The prepared porous silicon coated zero-valent iron material has a large specific surface area and the particle size is within the optimal size range (0.1-1[Mu]m) for colloidal particles to transfer through aquifer porous media, compared with uncoated zero-valent iron, the activity of the porous silicon coated zero-valent ironmaterial and the transfer ability in the aquifer porous media are both greatly improved, the disadvantage that the activity of nano-iron is prone to being weakened by the chemical composition of groundwater is overcome, secondary pollution and ecological toxicity caused by iron ion desolvation are reduced, and the preparation method can be used for the in-situ remediation of the groundwater pollution.

The invention discloses a preparation method of a monatomic catalyst for preparing synthesis gas by methane dry reforming , the preparation process adopts a one-step synthesis method, the active component metal Ni in the obtained catalyst is uniformly dispersed on a carrier CeO2 in an isolated atom form, the catalytic stability can be effectively improved, and the preparation method has the advantage of simple steps. The invention also discloses the monatomic catalyst prepared by the method, monatomic dispersed Ni has high catalytic activity, Ni is limited by oxygen vacancy of CeO2, and sintering agglomeration of Ni at high temperature can be effectively prevented; and the doped second metal regulates and controls the oxygen vacancy concentration on the surface of CeO2, a large number of oxygen vacancies can effectively activate carbon dioxide, surface active oxygen is generated, and the influence of carbon deposition on the activity of the catalyst is inhibited. The invention also discloses an application of the monatomic catalyst in preparation of synthesis gas by methane dry reforming, the catalyst can be continuously used for 150 hours without obvious deactivation, and a good technical effect is achieved.

The invention belongs to the field of biomedicines, and discloses a soluble thrombin nano-particle, and a preparation method and application thereof. The soluble thrombin nano-particle is of a core-shell structure, wherein the core is thrombin, and the shell is a nano-particle formed by crosslinking of cations of water soluble amylose-polylysine. The core-shell structure is advantageous to maintain the bioactivity of thrombin, can be preserved stably at room temperature, has the function of sustained release, is long in duration of drug-acting, cannot be easily degraded when entering a body, and is economical and safe. According to the nanoparticle, the particle diameter is 150-250nm preferably, so that the bioactivity of the thrombin can be well maintained, and the stability and sustained release function of nano-thrombin can be further improved. The encapsulation efficiency of the nano-thrombin is 50-75 percent preferably to well improve the stability and sustained release function of the nano-thrombin.

The invention belongs to the field of drug delivery systems of macromolecular drugs and in particular relates to lysozyme cross-linked dextran sustained-release microspheres and a preparation method thereof. The lysozyme cross-linked dextran sustained release microspheres are characterized by being prepared from the following components: cross-linked dextran microspheres and lysozyme. The preparation method comprises the following steps: weighing quantitative lysozyme and adding the lysozyme into purified water; after the lysozyme is completely dissolved, adding quantitative dextran microspheres into a solution; sufficiently stirring on a magnetic stirrer to completely disperse the microspheres; standing at room temperature and carrying out adsorption and drug loading; filtering and collecting the microspheres; freezing and drying in vacuum to obtain the lysozyme cross-linked dextran sustained-release microspheres. The sustained-release microspheres provided by the invention have good sustained-release effect, water swelling effect and biological adhesion effect and can be used for mucosal drug delivery and skin drug delivery so as to better express anti-inflammatory, antibacterial and anti-virus effects of the lysozyme.