The invention provides a preparation method of
mesenchymal stem cell fragmentation factor freeze-dried
powder with multiple tissue sources. According to the method, mesenchymal stem cells are separated from adipose tissues, mveloid tissues and
umbilical cord tissues of a
mammal, purified, and subjected to
mixed culture, in the
mixed culture process, supernate is collected and cultured in every stages, stem cells obtained through
mixed culture are subjected to ultrasonication,
stem cell fragmentation factors are collected, through two-time freeze-
drying concentration treatment, the
stem cell fragmentation factor freeze-dried
powder is prepared, the whole freeze-
drying process is maintained at 20 DEG C or below, heating is conducted step by step slowly, effects of temperature change on the activity of the
stem cell factors are prevented, in a low-temperature low-pressure environment,
crystal water slowly sublimates, in the presence of a freeze-
drying protective additive, other componentsof the stem
cell factors are not reduced, the activity is not affected, a compound which is easy to quantify and rich in mesenchymal stem bioactive factors is obtained, and the problem that when stemcell factors are concentrated,
cell factor type loss is caused by different molecular weights of the stem
cell factors is solved.