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pH-responsive magnetic metal organic framework composite nano-material and its preparation method and use

A composite nanomaterial, magnetic metal technology, applied in the application field of glycoprotein capture and release, can solve problems affecting protein analysis or application, etc.

Active Publication Date: 2017-04-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In protein-related applications, scientists mainly focus on improving the capture efficiency of proteins, while ignoring the release process of proteins. It is difficult for proteins captured by MOFs to be eluted from the material, even if they can be eluted, due to the The removal process needs to add substances that have an impact on protein activity, such as 2-methylimidazole or trifluoroacetic acid (see literature W.L.Liu, S.H.Lo, B.Singco, C.C.Yang, H.Y.Huang and C.H.Lin, J.Mater.Chem. B, 2013, 1,928; M. Zhao, C. Deng and X. Zhang, Chem. Commun., 2014, 50, 6228; N. Sun, X. Zhang and C. Deng, Nanoscale, 2015, 7, 6487; M .Zhao, X.Zhang and C.Deng, Chem.Commun., 2015, 51, 8116; Y.Chen, Z.Xiong, L.Peng, Y.Gan, Y.Zhao, J.Shen, J.Qian, L. Zhang and W. Zhang, ACS Appl. Mater. Interfaces, 2015, 7, 16338; J. Zheng, Z. Lin, G. Lin, H. Yang and L. Zhang, J. Mater. Chem. B, 2015, 5,2185.), will seriously affect the further analysis or application of the released protein

Method used

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  • pH-responsive magnetic metal organic framework composite nano-material and its preparation method and use
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  • pH-responsive magnetic metal organic framework composite nano-material and its preparation method and use

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Experimental program
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Embodiment 1

[0044] Embodiment 1 prepares Fe 3 o 4 / Polymer / MOFs Composite Nanomaterials

[0045] In this embodiment, the polymer layer is polyvinylpyrrolidone (PVP), the metal-organic framework uses iron nitrate as a metal source, and 1,4-terephthalenediboronic acid (PBA) as an organic ligand.

[0046] figure 1 gives Fe 3 o 4 / Polymer / MOFs composite nanomaterials preparation flow chart, firstly use the solvothermal method to prepare the surface negatively charged Fe 3 o 4 Nanoparticles; then according to the interaction of electrostatic interaction and van der Waals force, the obtained Fe 3 o 4 The surface of the nanoparticles is coated with a layer of polymer layer, and the Fe coated with the polymer layer is obtained. 3 o 4 Nanospheres (Fe 3 o 4 / Polymer nanoparticles); then ferric nitrate as the metal source, 1,4-terephenylboronic acid (PBA) as the organic ligand, and Fe 3 o 4 / Polymer nanoparticles together to prepare Fe by one-pot method 3 o 4 / Polymer / MOFs composite n...

Embodiment 2

[0056] Embodiment 2 prepares Fe 3 o 4 / Polymer / MOFs Composite Nanomaterials

[0057] In this embodiment, the polymer layer is polyvinylpyrrolidone (PVP) and polyetherimide (PEI), the metal-organic framework uses ferric nitrate as the metal source, and 1,4-terephenylboronic acid (PBA) as the organic ligand. body.

[0058] Fe in this example 3 o 4 / Polymer / MOFs composite nanomaterial preparation steps are as follows:

[0059] (1) Preparation of Fe 3 o 4 Nanoparticles

[0060] Add raw materials 1.157g ferric chloride hexahydrate, 0.4g sodium citrate and 3.303g ammonium acetate to a polytetrafluoroethylene stainless steel reaction kettle filled with 60mL solvent ethylene glycol, and magnetically stir for 1 hour to dissolve the above raw materials; then transfer Remove the stirrer, raise the temperature of the reactor to 200°C, and react for 16 hours; then cool the reactor to room temperature, and collect the product in the reaction solution obtained by the reaction with a ...

Embodiment 3

[0067] Embodiment 3 prepares Fe 3 o 4 / Polymer / MOFs Composite Nanomaterials

[0068] In this embodiment, the polymer layer is polydopamine (PDA) and polyacrylic acid (PAA), the metal-organic framework uses ferric chloride as the metal source, and 3-carboxyphenylboronic acid as the organic ligand.

[0069] The preparation steps of the Fe3O4 / Polymer / MOFs composite nanomaterial in this example are as follows:

[0070] (1) Preparation of Fe 3 o 4 Nanoparticles

[0071] Add raw materials 1.157g ferric chloride hexahydrate, 0.7g sodium citrate and 3.303g ammonium acetate to a polytetrafluoroethylene stainless steel reaction kettle filled with 60mL solvent ethylene glycol, and stir magnetically for 1 hour to dissolve the above raw materials; then transfer Remove the stirring bar, raise the temperature of the reactor to 220°C, and react for 17 hours; then cool the reactor to room temperature, and collect the product in the reaction liquid obtained by the reaction with a magnet; t...

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Abstract

The invention discloses a pH-responsive magnetic metal organic framework composite nano-material and its preparation method and use. The pH-responsive magnetic metal organic framework composite nano-material comprises Fe3O4 nanoparticles, polymer layers coating the surfaces of the Fe3O4 nanoparticles and a metal organic framework growing on the polymer layers. The polymer layer comprises a first polymer chelated with metal ions or comprises the first polymer chelated with metal ions and a hydrophilic second polymer. The metal organic framework is formed from Fe<3+> and a phenylboronic acid derivative containing at least one carboxyl group or at least two boronic acid groups through a coordination bond. The composite nano-material provided by the invention has good magnetic response performances and can realize reversible and highly selective capture and release of glycoprotein in different pH environments, and the pH environment is relatively mild so that the influence on the glycoprotein activity is avoided.

Description

technical field [0001] The invention belongs to the field of magnetic composite nanomaterials, and in particular relates to a pH-responsive magnetic metal-organic frame composite nanomaterial, a preparation method thereof and an application in glycoprotein capture and release. Background technique [0002] Protein glycosylation, that is, the transfer of sugar to proteins under the action of glycosyltransferases, and the formation of glycosidic bonds with amino acids on proteins, is the most common form of post-translational modification of proteins and plays an important role in many biological activities roles, such as cell adhesion, molecular recognition, signal transduction, protein folding, and metabolic pathways. Abnormal glycosylation is also associated with many diseases, such as diabetes, cancer, neurodegenerative diseases, cardiovascular diseases, etc. To better understand these biological processes, or to discover new disease markers, glycoproteins first need to b...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30C07K14/79C07K14/47C12N9/08
CPCB01J20/06B01J20/226B01J20/26B01J20/28009C07K14/4715C07K14/79C12N9/0065C12Y111/01007
Inventor 蓝芳杨琦吴尧顾忠伟
Owner SICHUAN UNIV
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