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Acidophilic bioleaching microorganism inclusion and preparation method thereof

A technology of microorganisms and embeddings, which is applied in the direction of fixing on/in the organic carrier, which can solve the problems that it is difficult to take into account the continuous output of high-activity and high-cell-concentration microbial solutions, and the easy loss of microorganisms, so as to increase the expansion rate of fillers , not easy to lose, maintain a large amount of effect

Active Publication Date: 2019-03-22
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide an embedding body for acidophilic ore leaching microorganisms and its preparation method, which solves the problem that microorganisms are easy to lose during continuous culture, and the existing immobilization technology is difficult to balance high activity and The problem of continuous output of high cell concentration microbial solution

Method used

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  • Acidophilic bioleaching microorganism inclusion and preparation method thereof
  • Acidophilic bioleaching microorganism inclusion and preparation method thereof
  • Acidophilic bioleaching microorganism inclusion and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A method for immobilizing microorganisms, comprising the steps of:

[0034] Step 1. Weigh 10g of sodium alginate, add 500mL of distilled water after mixing, and heat it in a microwave oven to dissolve completely. When the temperature is cooled to 50°C, add 3g of micro-nano potassium carbonate with an average particle size of 266nm, and stir evenly to obtain an embedding suspension Solution A, keep warm at 45°C;

[0035] In step 2, 10 L of cultured Leptospirillum ferriphilum YSK and Sulfobacillus thermosulfidooxidans YN22 were centrifuged at 10,000 × g for 20 minutes in a high-speed centrifuge to collect the sludge, and used 500L of dilute sulfuric acid suspended bacteria sludge with pH 2.0 to obtain 3.6×10 9 Individual / mL microbial suspension B;

[0036] Step 3, weigh 20g of calcium chloride, dissolve it in distilled water, and set the volume to 500mL to obtain cross-linking solution C;

[0037] Step 4, weigh 1.5g of ammonium sulfate, 0.25g of magnesium sulfate, 0.25...

Embodiment 2

[0043] A method for immobilizing microorganisms, comprising the steps of:

[0044] Step 1: Weigh 20g of polyvinyl alcohol and 30g of sodium alginate respectively, add 1L of distilled water after mixing, heat in a microwave oven to dissolve completely, and when the temperature cools down to 50°C, add 5g of sodium carbonate powder, the particle size of which is less than 5μm, and stir Obtain embedding suspension A evenly and keep it warm at 30°C;

[0045] Step 2: Concentrate 50L of cultured Acidithiobacillus ferrooxidans ATCC23270 through a ceramic membrane reactor with a pore size of 0.1 μm to 5L, and then centrifuge the concentrated bacterial solution through a high-speed centrifuge at 10,000×g for 20 minutes to collect the sludge , with 1L of dilute sulfuric acid at pH 2.0 to suspend the sludge to obtain 1.3×10 10 Individual / mL microbial suspension B;

[0046] Step 3, weigh 30g of calcium chloride and 25g of boric acid, dissolve them in distilled water, and set the volume t...

Embodiment 3

[0054] A method for immobilizing microorganisms, comprising the steps of:

[0055] Step 1, weigh 5g of polyvinyl alcohol, 5g of polyoxyethylene and 10g of sodium alginate, mix and add 1L of distilled water, heat it in a microwave oven to dissolve completely, when the temperature is cooled to 50°C, add 5g of nano-sodium bicarbonate powder , the average particle size is about 280nm, stir evenly to obtain embedding suspension A, keep warm at 45°C;

[0056] Step 2: Concentrate 50L of the cultured Sulfobacillus acidophilus TPY to 5L through a ceramic membrane reactor with a pore size of 0.1 μm, and then centrifuge the concentrated bacterial solution through a high-speed centrifuge at 10,000×g for 20 minutes to collect the sludge , with 1L of dilute sulfuric acid at pH 2.0 to suspend the sludge to obtain 2.1×10 10 Individual / mL microbial suspension B;

[0057] Step 3, weigh 30g of calcium chloride and 20g of boric acid, dissolve them in distilled water, and set the volume to 1L to...

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Abstract

The invention belongs to the technical field of microbial immobilization and particularly relates to an acidophilic bioleaching microorganism inclusion and a preparation method thereof. The acidophilic bioleaching microorganism inclusion adopts a net stricture formed through crosslinking on the surface layer of a polyurethane porous hydrophilic filler carrier and is prepared from polyurethane porous hydrophilic filler, an inclusion agent, carbonate, an acidophilic bioleaching microorganism suspension, a crosslinking agent and a stabilizer, wherein coronate is one or more of sodium carbonate, sodium bicarbonate, potassium carbonate and potassium bicarbonate; the stabilizer is a microorganism culture solution containing polyvalent metal ions; the pH of the acidophilic bioleaching microorganism suspension is smaller than 3.0. The preparation method of the inclusion comprises step of sequentially immersing the hydrophilic filler expanding with water into the suspension containing an inclusion solution and carbonate, the microorganism suspension, a crosslinking solution and a stabilization solution. The problems that conventional inclusions have poor vapor permeability and continuous production of microorganism solutions with high activity and high cell concentration in the culture process cannot be considered are solved.

Description

technical field [0001] The invention belongs to the technical field of microorganism immobilization, and in particular relates to an acidophilic ore leaching microbial embedding body and a preparation method thereof. Background technique [0002] Biometallurgy is a technology formed by dissolving valuable metals in minerals in the form of ions into leachate and recovering them under the catalytic oxidation of relevant microorganisms, or dissolving and removing harmful elements in minerals, combined with hydrometallurgy and other related processes . The leaching of elements in minerals by biometallurgy has been discovered for nearly a hundred years, but its basic theoretical research is relatively weak and has not been developed until the middle and late 20th century, when the technology finally got a certain development. The huge advantages of biometallurgy have led to the rapid development of this technology. At present, biometallurgy has been widely used in the mining and...

Claims

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Application Information

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IPC IPC(8): C12N11/08
CPCC12N11/08
Inventor 周洪波周文博程海娜
Owner CENT SOUTH UNIV
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