Immune cell cryopreservation solution and immune cell cryopreservation method

A technology of immune cells and cryopreservation method, applied in the field of immune cell cryopreservation, to achieve the effect of simple formula, high clinical safety, and unaffected activity

Inactive Publication Date: 2020-07-10
GUANGDONG XIANKANGDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an immune cell cryopreservation and immune cell cryopreservation method, which can effectively solve the problem of long-term storage of immune cells, long-term transportation, flexibility of use, etc.

Method used

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  • Immune cell cryopreservation solution and immune cell cryopreservation method
  • Immune cell cryopreservation solution and immune cell cryopreservation method

Examples

Experimental program
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Effect test

Embodiment 1

[0044]PBMC cryopreservation and recovery of peripheral blood mononuclear cells:

[0045] An immune cell cryopreservation solution and an immune cell cryopreservation method, the specific steps are as follows:

[0046] Prepare immune cell cryopreservation solution, including A cryopreservation solution and B cryopreservation solution, whose components are as follows:

[0047] A freezing medium:

[0048] Human Albumin 10ml

[0049] Cell Stabilizer 3ml

[0050] KBM581 serum-free medium 87ml

[0051] Prepare A freezing solution according to the above components, and store it in a refrigerator at 4°C for later use;

[0052] B cryopreservation solution:

[0053] Medical DMSO 20ml

[0054] KBM581 serum-free medium 80ml

[0055] Prepare B cryopreservation solution according to the above components, and store it in a refrigerator at 4°C for later use;

[0056] Use the above prepared immune freezing medium to freeze peripheral blood mononuclear cells PBMC:

[0057] 1. Take huma...

Embodiment ２

[0063] Cryopreservation and recovery of umbilical cord blood mononuclear cell PBMC and culture of CIK and NK

[0064] An immune cell cryopreservation solution and an immune cell cryopreservation method, the specific steps are as follows:

[0065] Prepare immune cell cryopreservation solution, including A cryopreservation solution and B cryopreservation solution, whose components are as follows:

[0066] A freezing medium:

[0067] Human Albumin 14ml

[0068] Cell Stabilizer 6ml

[0069] KBM581 serum-free medium 80ml

[0070] Prepare A freezing solution according to the above components, and store it in a refrigerator at 4°C for later use;

[0071] B cryopreservation solution:

[0072] Medical DMSO 16ml

[0073] KBM581 serum-free medium 84ml

[0074] Prepare B cryopreservation solution according to the above components, and store it in a refrigerator at 4°C for later use;

[0075] Umbilical cord blood mononuclear cell PBMCs are cryopreserved using the above-prepared imm...

Embodiment 3

[0083] Immune cell CIK cryopreservation and recovery

[0084] An immune cell cryopreservation solution and an immune cell cryopreservation method, the specific steps are as follows:

[0085] Prepare immune cell cryopreservation solution, including A cryopreservation solution and B cryopreservation solution, whose components are as follows:

[0086] A freezing medium:

[0087] Human Albumin 20ml

[0088] Cell Stabilizer 10ml

[0089] KBM581 serum-free medium 70ml

[0090] Prepare A freezing solution according to the above components, and store it in a refrigerator at 4°C for later use;

[0091] B cryopreservation solution:

[0092] Medical DMSO 10ml

[0093] KBM581 serum-free medium 90ml

[0094] Prepare B cryopreservation solution according to the above components, and store it in a refrigerator at 4°C for later use;

[0095] The umbilical cord blood mononuclear cells and the CIK cells amplified and cultured by the resuscitated umbilical cord blood mononuclear cells in...

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Abstract

The invention provides an immune cell cryopreservation solution and an immune cell cryopreservation method, which are characterized in that the immune cell cryopreservation solution is composed of twoindependent cryopreservation solutions, namely, a cryopreservation solution A and a cryopreservation solution B, and the two cryopreservation solutions are mixed for use according to a ratio of 1: 1(v / v); and when immune cells are cryopreserved by using the immune cell cryopreservation solution, the immune cells are re-suspended first by using the cryopreservation solution A, the cryopreservation solution B with the same volume is then added, the mixed solution is uniformly blown and beaten and sub-packaged into cryopreservation containers, and the cryopreservation containers are transferredinto an ultralow-temperature refrigerator and then transferred into liquid nitrogen for storage after 24 hours. Compared with the existing cell cryopreservation technology, the immune cell cryopreservation solution and the immune cell cryopreservation method have the advantages that immune cells are effectively protected, the operation is simple, the cryopreservation effect is good, the immune cells are not damaged in the cryopreservation process, the safety is high, the physiological function and biological characteristics of the recovered immune cells are guaranteed, the completeness of surface antigens of the immune cells is guaranteed, and the survival time of the immune cells is prolonged.

Description

technical field [0001] The present invention relates to the technical field of cryopreservation of immune cells, and more specifically, to a liquid for cryopreservation of immune cells and a method for cryopreservation of immune cells. Background technique [0002] Cell cryopreservation is a technology that places cells in a low temperature environment to slow down cell metabolism to achieve long-term storage. The principle is to store the cells at -196°C in liquid nitrogen at ultra-low temperature, which can make the cells temporarily out of the growth state and preserve the cell characteristics, and can be used by resuscitating the cells when needed. Adding a protective agent to the cell suspension during cryopreservation can lower the freezing point of the solution. Under the condition of slow freezing, the water in the cells penetrates out of the cells, reducing the formation of ice crystals in the cells, thereby avoiding damage to the cells during freezing. [0003] Ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 谢海涛薛卫巍方晓王乃会钟家炜谢天仲
Owner GUANGDONG XIANKANGDA BIOTECH CO LTD
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