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Preparation of epsilon-poly-L-lysine component with high antibacterial activity

A technology of antibacterial activity and lysine, which is applied in the field of preparation of high antibacterial activity ε-poly-L-lysine components, and can solve the problems of differences in antibacterial effects

Active Publication Date: 2009-07-22
ZHEJIANG SILVER ELEPHANT BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Studies have shown that the molecular weight distribution of ε-PL produced by microbial fermentation is wide, and ε-PL with different degrees of polymerization has different physical and chemical properties and uses; in addition, the molecular weight of ε-PL is different, and its antibacterial effect is very different

Method used

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  • Preparation of epsilon-poly-L-lysine component with high antibacterial activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Content 20g / Lε-PL fermentation broth is centrifuged to separate bacteria, and the solution obtained after decolorization can be separated by membrane combination separation device.

[0026] Take 2000mlε-PL filtrate and pass through 0.2μm microfiltration membrane, 25KDa, 10KDa, 5KDa, 2KDa, 1KDa ultrafiltration membrane and nanofiltration membrane step by step. Among them, the operating pressure of microfiltration is 0.05MPa, the operating pressure of ultrafiltration membrane with molecular weight cut off of 25KDa, 10KDa and 5KDa is 0.15MPa, the operating pressure of ultrafiltration membrane with molecular weight cut off of 2KDa is 0.2MPa, and the operating pressure of ultrafiltration membrane with molecular weight cut off of 1KDa is 0.2MPa. The operating pressure is 0.7MPa.

[0027] The rejection rate of the microfiltration membrane to ε-PL filtrate is 0, and the volume of the permeate is about 2000ml; after passing through the 25KDa ultrafiltration membrane, the volume ...

Embodiment 2

[0031] The ε-PL product powder is prepared into 2L of 20g / L solution (pH is neutral, temperature is 25°C), and then separated by membrane filtration through microfiltration, ultrafiltration and nanofiltration combined membrane separation device in sequence. Among them, the operating pressure of microfiltration is 0.1MPa, the operating pressure of ultrafiltration membrane with molecular weight cut off of 25KDa, 10KDa and 5KDa is 0.2MPa, the operating pressure of ultrafiltration membrane with molecular weight cut off of 2KDa is 0.25MPa, and the operating pressure of ultrafiltration membrane with molecular weight cut off of 1KDa is 0.25MPa. The operating pressure is 1MPa.

[0032] The above 2000ml ε-PL solution completely permeated the microfiltration membrane; after that, it passed through the 25KDa ultrafiltration membrane, and the volume ratio of the permeate to the retentate was about 10:1; then passed through the 10KDa ultrafiltration membrane, the permeate to the retentate ...

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Abstract

The invention relates to a method for preparing a high antibacterial activity Epsilon-poly-L-lysine (Epsilon-PL) component; the high antibacterial activity component Epsilon-PL product is obtained by using micro-filtration, ultrafiltration and nanofiltration combination multi-level eparation technology for obtaining Epsilon-PL with different molecular weight distribution, determining the molecular weight range of the optimum antibacterial activity component by bacteriostasis experiment, and further being collected and dried. By using the method, the high-purity Epsilon-PL with different molecular weight ranges can be obtained; and as a membrane separation technology is adopted, any effect on the activity of Epsilon-PL is not caused, thus being favorable to research of the Epsilon-PL with different molecular weight.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, in particular to a preparation method of ε-poly-L-lysine component with high antibacterial activity. Background technique [0002] ε-poly-L-lysine (ε-poly-L-lysine, referred to as ε-PL) is an amino acid homopolymer biosynthesized by Streptomyces albicans and other strains. The ε-amino group of the acid is connected with the amide bond formed by the α-carboxyl group of another monomer L-lysine. ε-PL has good water solubility, slightly soluble in ethanol, high thermal stability, broad antibacterial spectrum, and can resist the activity of Gram-positive bacteria, Gram-negative bacteria, fungi, heat-resistant bacillus and viruses. Break down into L-lysine. L-Lysine is one of the eight essential amino acids for the human body, so it is a safe and efficient natural biological preservative. In addition, ε-PL can also be used as a gene transport carrier, drug coating material, electronic ...

Claims

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Application Information

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IPC IPC(8): C08G69/08C08G69/46
Inventor 贾士儒沈颜新谭之磊周斌贾原媛范宝庆
Owner ZHEJIANG SILVER ELEPHANT BIO ENG
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