Preparation of epsilon-poly-L-lysine component with high antibacterial activity
A technology of antibacterial activity and lysine, which is applied in the field of preparation of high antibacterial activity ε-poly-L-lysine components, and can solve the problems of differences in antibacterial effects
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[0024] Example 1:
[0025] The content of 20g / Lε-PL fermentation broth is centrifuged to separate the bacteria, and the solution obtained after decolorization can be separated by a combined membrane separation device.
[0026] Take 2000ml ε-PL filtrate, step by step through 0.2μm microfiltration membrane, 25KDa, 10KDa, 5KDa, 2KDa, 1KDa ultrafiltration membrane and nanofiltration membrane. The operating pressure of microfiltration is 0.05MPa, the operating pressure of ultrafiltration membranes with molecular weight cut-off of 25KDa, 10KDa, and 5KDa is 0.15MPa, and the operating pressure of ultrafiltration membranes with molecular weight cut-off of 2KDa is 0.2MPa. The operating pressure is 0.7MPa.
[0027] The rejection rate of the ε-PL filtrate by the microfiltration membrane is 0, and the volume of the permeate is about 2000ml; after passing through the 25KDa ultrafiltration membrane, the volume ratio of the permeate to the concentrated solution is about 10:1; after passing throug...
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[0030] Example 2:
[0031] The ε-PL product powder is configured into 2L of 20g / L solution (neutral pH, temperature 25℃), and then the membrane is separated by membrane filtration through a combined membrane separation device of microfiltration, ultrafiltration and nanofiltration in sequence. The operating pressure of microfiltration is 0.1MPa, the operating pressure of ultrafiltration membranes with molecular weight cut-off of 25KDa, 10KDa, and 5KDa is 0.2MPa, the operating pressure of ultrafiltration membranes with molecular weight cut-off of 2KDa is 0.25MPa, and the operating pressure of nanofiltration membranes with 1KDa The operating pressure is 1MPa.
[0032] The above 2000ml ε-PL solution completely penetrates the microfiltration membrane; afterwards, it passes through the 25KDa ultrafiltration membrane, the volume ratio of the permeate to the retentate is about 10:1; then passes through the 10KDa ultrafiltration membrane, the permeate and the retentate The volume ratio of ...
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