Heart acellular matrix composite temperature-sensitive gel and application thereof

Abstract

The invention relates to a heart acellular matrix composite temperature-sensitive gel, which is characterized in that the composite temperature-sensitive gel is composed of a chitosan temperature-sensitive gel and pig heart acellular matrix particles (the particle size is 10-100 [mu] m). The invention also provides a preparation method of the composite temperature-sensitive gel, which comprises the following steps: (1) preparing a chitosan solution, and dropwise adding a beta-sodium glycerophosphate solution into the chitosan solution under ice bath and stirring conditions to prepare a low-temperature chitosan temperature-sensitive gel gelling solution; (2) preparing pig heart acellular matrix particles, wherein the particle size is 10-100 [mu] m; (3) adding pig heart acellular matrix particles into the low-temperature chitosan temperature-sensitive gel forming solution, wherein the particle concentration is 0.2-6% (w/v, g/ml), and thus obtaining a heart acellular matrix composite temperature-sensitive gel forming solution; and (4) quickly converting the low-temperature cardiac acellular matrix composite temperature-sensitive gel forming solution into a gel state under the condition of 33-37 DEG C. The gel-forming solution of the heart acellular matrix composite temperature-sensitive gel can reach the damaged part of the heart through injection, composite gel is rapidly formed in situ, and the effect of repairing the damaged cardiac muscle tissue is achieved.

Description

technical field [0001] The invention relates to the field of regenerative medicine, in particular to a heart decellularized matrix composite temperature-sensitive gel and its application. Background technique [0002] Injectable thermosensitive gel is liquid at low temperature and turns into hydrogel at 37°C. It is suitable as a delivery carrier for in vivo injection therapy and effectively avoids secondary damage to the body, so it has broad clinical application prospects. Chitosan comes from the shells of crabs and shrimps. It has a wide range of sources, is non-toxic, has excellent biocompatibility and degradability, and has been used clinically as a biological material. Chitosan is insoluble in neutral and alkaline environments, and can be dissolved in acidic solutions such as hydrochloric acid and acetic acid, but when the pH value exceeds about 6.2, precipitation will occur. However, when using β-glycerophosphate sodium solution to neutralize chitosan, a mixed solutio...

AI Technical Summary

Problems solved by technology

In order to overcome the defect of low myocardial tissue affinity of chitosan thermosensitive gel, the present invention discloses a preparation method of cardiac decellularized matrix composite thermosensitive gel, that is, by adding porcine heart decellularized gel into the gelling solution of chitosan thermosensitive gel Cell matrix particles can significantly improve the affinity of myocardial tissue and accelerate the repair of damaged myocardial tissue, which has a very important application prospect