102 results about "Cardiac muscle tissue" patented technology

Coronary sinus ring apparatus, including an elongated body for deployment in a coronary sinus, an aperture in a long side of the body, at least a partial lumen extending from one end of the elongated body to at least the aperture, and a guide within the elongated body positioned and shaped to guide an element inserted along the lumen to the aperture. Apparatus for treating a heart, including a delivery tube sized for and adapted for insertion into a body, a sharp tip adapted to be pushed through cardiac muscle, an elongate tensioning element, and a foldable anchor adapted to couple the elongate tension element to cardiac muscle tissue, wherein the delivery tube encloses one or both of the elongate tensioning element and the foldable anchor. Related apparatus is also described.

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and/or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and/or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

The invention belongs to the field of researching and application of biomedicine and particularly relates to human pluripotent stem cell-hereditary cardiomyopathy cardiac muscle cells and a preparation method thereof. The invention provides a human hereditary cardiomyopathy-pluripotent stem cell, which is constructed by means of TALEN or CRISPR/CAS9 genome editing technology. The human hereditary cardiomyopathy cardiac muscle cell can be combined with any scaffold materials to culture various in-vitro human hereditary cardiomyopathy cardiac muscle tissues. The human hereditary cardiomyopathy cardiac muscle cells in the invention have disease phenotypes and electrophysiology change being similar as the cardiac muscle cells of human hereditary cardiomyopathy patients. The human hereditary cardiomyopathy cardiac muscle cells are wide in sources and can be cultured in-vitro for a long time. The invention provides excellent tools and platforms for researching an effective new therapy means and researching an effective corresponding treatment medicine.

This invention provides a catheter used for blocking abnormal conduction in the cardiac muscle using photodynamic therapy or treating arrhythmia and a method for evaluating the therapeutic effects of the catheter. The catheter has a structure that freely bends at its end, which is used for performing photodynamic ablation of cardiac muscle tissue via photochemical reactions in the blood vessel or cardiac lumen. The catheter comprises a light-emitting window for applying a light beam transmitted through an optical fiber to a target site of cardiac muscle tissue and at least two electrodes for potential measurement in the periphery of the light-emitting window.

The invention discloses a use of a derived polypeptide series of pigment epithelium derived factors in ischemic myocardium protection. The polypeptide is derived from the pigment epithelium derived factors, and every amino acid sequence in the polypeptide includes at least eighteen continuous amino acid residues. The PEDF derived functional peptide has good function advantages, and has remarkablecardiac muscle tissue capillary tube stabilization, vascular leakage prevention, myocardial cell protection and cardiac muscle tissue inflammation prevention effects.

The invention discloses a two-photon fluorescence probe for detecting peroxynitrite as well as a preparation method and application of the two-photon fluorescence probe. The two-photon fluorescence probe has the structural formula as shown in the description. The two-photon fluorescence probe is named as TPNIR-FP. The two-photon fluorescence probe adopts a Nile red derivative as a fluorescent group and an alpha-keto-amide functional group as an ONOO-reaction group, can rapidly respond to ONOO- (within 10 seconds), and in addition is high in sensitivity and good in selectivity. The two-photon fluorescence probe can be applied to imaging research on ONOO- in antharcycline antibiotic induced cardiac muscle cell and cardiac muscle tissue damage.

[Summary]

[Object]

To develop a preparation and a treatment method effective for improving a state of a bald hear, promoting hair growth, and restoring injuredskin tissues, supporting tissues, and cardiac muscle.

[Solution]

A preparation containing atrial diuretic hormone family molecules are used for restoration and regeneration of a tissue and an organ. The preparation is used for growing hairs and/or for improving and restoring failures of skin, mucosa, and subcutaneous tissue such as bed bore, rough and dry skin, burn injuries, necrosis, and psoriasis, and also for improving cardiac muscle failures.

The invention belongs to the field of biomedical engineering, and relates to a human tissue-engineered cardiac muscle tissue and a preparation method thereof. The human tissue-engineered cardiac muscle tissue disclosed by the invention is prepared by inoculating all kinds of cell components of a normal heart differentiated from human induced multipotential stem cells by taking a natural decellularized heart substrate as the scaffold. The human tissue-engineered cardiac muscle tissue has cell components, extracellular matrixes and biological functions similar to that of a normal human cardiac muscle tissue; testing and screening of in-vitro drugs, initial in-vivo transplantation researches of clinical earlier-stage small animals and observation of treatment effects can be carried out; and helps can be finally offered for realizing individualized treatment of human cardiovascular diseases.

The invention discloses a chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and belongs to the technical field of organ chips. The chip device comprises a PDMS (Polydimethylsiloxane) substrate, wherein the PDMS substrate is provided with two cell culture cavities for culturing cardiac muscle cells at interval; biological electrodes are respectively and correspondingly arranged in the cell culture cavities, and the two cell culture cavities are communicated; the biological electrodes connect chips and external electric stimulation device or electric-signal acquisition device respectively by inputting or outputting of electric signals, and carry out electric stimulation on the cardiac muscle tissues or real-time acquisition on electricsignals of cardiac muscle tissues. The chip device disclosed by the invention has the beneficial effects that the cardiac muscle cells are inoculated in the culture cavities and can grow and be anchored on two electrode masts to realize in-vitro three-dimensional culture of the cardiac muscle cells, so that the two electrodes are utilized for electrophysiological-signal acquisition and electrical-stimulation experiment and the like, and further the applications of drug screening and environmental-poison evaluation and the like can be carried out.

The invention provides a method for preparing a cardiac muscle tissue engineering decellularization collagen diaphragm. The method comprises the following step: selecting natural pork tendon tissue, and performing decellularization on the tissue by using treatment methods of repeated freezing and thawing and nuclease treatment, thereby obtaining decellularization pork tendon diaphragms of different thicknesses, wherein the thicknesses of the diaphragms are within 5-30[mu] m; the obtained diaphragms comprise collagen as a main component; and the diaphragms are of structures of fibers which are arranged in parallel, are similar to extracellular matrix components and structures of cardiac muscle tissue cells, can induce cardiac muscle cells to extend along the fiber direction, and thus can be used for culturing cardiac muscle cells and establishing heart-like tissue. The method has the advantages of being convenient in material obtaining, low in price, stable in preparation process, uniform in product morphology, and simple and gentle, and collagen diaphragms can be highly controlled, have complete structures, morphology, biological properties and inherent mechanical properties of materials, furthermore have good biocompatibility and biodegradability, and are very applicable to cardiac muscle tissue engineering construction and regenerative medicine.

The invention discloses a dual-pole cardiac muscle tissue one-way action potential recording electrode. The dual-pole cardiac muscle tissue one-way action potential recording electrode comprises a puncture needle rod, lower endocardium cardiac muscle recording electrode installation holes and middle layer cardiac muscle recording electrode installation holes are formed in the outer wall of the puncture needle rod, a pair of lower endocardium cardiac muscle recording electrode wires and a pair of middle layer cardiac muscle recording electrode wires are arranged in an inner cavity of the puncture needle rod, the front ends of the lower endocardium cardiac muscle recording electrode wires are fixed to the lower endocardium cardiac muscle recording electrode installation holes, the front ends of the middle layer cardiac muscle recording electrode wires are fixed to the middle layer cardiac muscle recording electrode installation holes, and the back ends of the lower endocardium cardiac muscle recording electrode wires and the back ends of the middle layer cardiac muscle recording electrode wires are connected with one ends of four transmission leads; a rigid positioning sleeve is arranged on the outer wall of the puncture needle rod, lower epicardium cardiac muscle recording electrode installation holes are formed in the rigid positioning sleeve, a pair of lower epicardium cardiac muscle recording electrode wires are arranged in the lower epicardium cardiac muscle recording electrode installation holes, the front ends of the lower epicardium cardiac muscle recording electrode wires are formed in the lower epicardium cardiac muscle recording electrode installation holes, and the back ends of the lower epicardium cardiac muscle recording electrode wires are connected with one ends of another two transmission leads. The one-way action potential of three layers of myocardial cells can be recoded at the same time, and the dual-pole cardiac muscle tissue one-way action potential recording electrode is ingenious in structural design, and easy and convenient to use.

The invention discloses a marker used for assisted detection of abrupt highland advancing low pressure low oxygen environment related myocardial damages, and applications of a product used for detection of miR-144-3p expression level in preparation of kits used for auxiliary diagnosis of myocardial damages caused by low pressure low oxygen. According to the applications, a low-pressure oxygen cabin is adopted for stimulation of highland low pressure low oxygen environment, and establishment of rat animal models with low pressure low oxygen myocardial damages. High flux sequencing technology and bioinformatics methods are adopted, key miRNA related to highland low pressure low oxygen myocardial damages are screened, and fluorogenic quantitative PCR is adopted for experiment verifying of thekey miRNAs in cardiac muscle tissues and blood plasma. The miRNAs disclosed in the invention can be used for assisted detection of abrupt highland advancing low pressure low oxygen environment related myocardial damages, and possess important meaning in diagnosis and assessment of abrupt highland advancing low pressure low oxygen environment related myocardial damages.

The invention discloses application of EMD638683 in protection of the heart suffering from acute myocardial infarction. The invention provides application of EMD638683 in preparation of products having the following functions: 1) prevention and/or treatment of myocardial infarction of animals; 2) prevention and/or treatment of heart failure caused by myocardial infarction of animals; and 3) inhibition of the expression of SGK1 protein in cardiac muscle tissue after myocardial infarction of animals. Experimental results of the invention prove that EMD638683 has treatment effect on mice with myocardial infarction, and experimental data is provided for clinical application of EMD638683 in treatment of myocardial infarction. The EMD638683 is of important theoretical significance to research on and development of medicines used for preventing and treating heart failure caused by myocardial infarction and has good application prospects.

The invention relates to a traditional Chinese medicine formula, in particular to a traditional Chinese medicine formula for treating heart qi deficiency and aims to solve the technical problems that the existing medicine for treating the heart qi deficiency has a great side effect, cures the symptoms not the disease, is not scientific in compatibility and is bad in treatment effect. The traditional Chinese medicine formula for treating the heart qi deficiency comprises the following raw materials in parts by weight: 8-12 parts of white ginseng, 8-12 parts of cassia twig, 13-17 parts of mongolian snakegourd, 13-17 parts of allium macrostemon, 8-12 parts of rhizoma pinelliae preparata, 13-17 parts of root of common peony, 13-17 parts of radix ophiopogonis and 3-7 parts of coptis chinensis. The traditional Chinese medicine formula for treating the heart qi deficiency, disclosed by the invention, can effectively improve the cardiac function of a model rat suffering from the heart qi deficiency, promotes recovery of scathing cardiac muscle tissues, adjusts biochemical criterion, and has an excellent treatment effect on the model rat suffering from the heart qi deficiency; as proved by clinical trial, the traditional Chinese medicine formula has a total effective rate of more than 90% in treating the heart qi deficiency, is free from toxic and side effects, and is used in the field of treating heart qi deficiency syndrome.

The invention provides an integrated anchor which comprises a locking rod, a locking sleeve arranged outside the locking rod in a sleeving mode and an anchoring piece. The anchoring piece is connected with the far end of the locking rod or the far end of the locking sleeve; a radial gap is formed between the locking rod and the locking sleeve; the locking rod is provided with a threading hole for threading artificial chordae tendineae; at least one end of the threading hole is communicated with the radial gap between the locking rod and the locking sleeve; the locking rod and the locking sleeve move relatively, so that the artificial chordae tendineae is locked in the radial gap between the locking rod and the locking sleeve; and the anchoring piece is used for being anchored into cardiac muscle tissues. The integrated anchor provided by the invention integrates the functions of anchoring and locking the artificial chordae tendineae, and when the integrated anchor is applied to a chordae tendineae repair operation, the surgical operation can be simplified, the number of instruments is reduced, and the cost is reduced.

The invention relates to an exosome derived from a mesenchymal stem cell. The exosome is prepared by intermittent hypoxic stimulation of the mesenchymal stem cell. The exosome prepared from the invention has stronger tissue and organ ischemia-reperfusion injury protective effect, and the biological effect of unit exosomes is improved, and treatment of diseases such as ischemic heart disease is more facilitated.

The invention relates to a preparation method of a three-dimensional histioid cardiac muscular tissue for studying a simulated microgravity effect, in particular relating to a preparation method of a three-dimensional histioid cardiac muscular tissue microballoon, aiming at solving the problems that according to the current cell three-dimensional culture method for studying the simulated microgravity effect, the cytodex microcarrier material is toxic and is not conducive to generation of cardiocytes, a great number of cells are needed for culture, cell harvesting is difficult, the cells are prone to interference of shearing force when being cultured in a klinostat and the continual contraction time of the cardiocytes is short. The method comprises the following steps: preparing a mixed solution; preparing cardiocytes; preparing a cell suspension, adding the cell suspension to a divalent positive ion solution to obtain microballoons; culturing the mircoballons in a culture medium to form the three-dimensional histioid cardiac muscular tissue. The three-dimensional histioid cardiac muscular tissue prepared by the method prepared by the invention can be cultured on a long-term basis,needs no passage and can maintain contraction of the cardiac muscular tissue for a long time. The method is applied to the research fields of space biology, aerospace medicine and tissue engineering.

The invention relates to the technical field of medical apparatuses, and in particular to a vascular radio-frequency ablation electrode catheter provided with claw-shaped catheter branches; a radio-frequency ablation working section is arranged on the front section of the vascular radio-frequency ablation electrode catheter , a catheter body section is arranged on the middle section of the vascular radio-frequency ablation electrode catheter and a control handle section is arranged on the rear section of the vascular radio-frequency ablation electrode catheter; the eight radio-frequency catheter branches extend from a peripheral direction of the radio-frequency ablation working section; the eight radio-frequency catheter branches are integrally represented as claw-shaped structures; and at least one ablation electrode is connected to the head end of each radio-frequency catheter branch. The vascular radio-frequency ablation electrode catheter provided by the invention has the beneficial effects that in practical clinical treatment, the vascular radio-frequency ablation electrode catheter provided with the claw-shaped catheter branches provided by the invention can be applied to various blood vessels differing in diameter and can be used for conducting radio-frequency ablation therapy on cardiac muscle tissues in a complex part, such as for vascular ablation of such diseases as paroxysmal atrial tachycardia, supraventricular tachycardia, atrial flutter, atrial fibrillation, resistant hypertension and the like.