Chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and application thereof

A three-dimensional culture and real-time monitoring technology, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, methods for supporting/immobilizing microorganisms, etc. It can solve myocardial damage and cannot monitor real-time dynamic changes of myocardial tissue for a long time, etc. question

Active Publication Date: 2018-08-07
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, this optical monitoring method needs to take the whole device out of the constant temperature incubator, and use a microscope for "indirect" observation and recording. It cannot monitor the real-time dynamic changes of myocardial tissue for a long time, and long-term observation will have certain damage to the myocardium. damage

Method used

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  • Chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and application thereof
  • Chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and application thereof
  • Chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and application thereof

Examples

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Embodiment 1

[0031] Example 1 Study on three-dimensional growth of cardiomyocytes on a chip

[0032] like figure 1 As shown, the material of the substrate 1 and the cell culture chamber 2 is PDMS, the shape of the culture chamber 2 is dumbbell-shaped, the length is 8 mm, the width of the middle cell growth channel is 0.5 mm, and the height is 1 mm; the material of the bioelectrode 3 is platinum -PDMS composite, wherein the volume fraction of platinum content is 16%-22%, the obtained electrode has better conductivity (lower impedance), correspondingly, at 10Hz, the impedance of the platinum-PDMS composite is in the range of 16-2KΩ Inside; Cardiomyocytes derived from neonatal rats can be inoculated on the chip. In order to cultivate three-dimensional myocardial tissue, the number of cells inoculated in the culture room is 1.5×10 6 -2.5×10 6 range, such as figure 2 As shown in the pictures taken by the laser confocal microscope (CLSM), the three-dimensional culture of myocardial tissue on...

Embodiment 2

[0033] Example 2: Research on drug screening of cardiomyocytes on a chip

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Abstract

The invention discloses a chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and belongs to the technical field of organ chips. The chip device comprises a PDMS (Polydimethylsiloxane) substrate, wherein the PDMS substrate is provided with two cell culture cavities for culturing cardiac muscle cells at interval; biological electrodes are respectively and correspondingly arranged in the cell culture cavities, and the two cell culture cavities are communicated; the biological electrodes connect chips and external electric stimulation device or electric-signal acquisition device respectively by inputting or outputting of electric signals, and carry out electric stimulation on the cardiac muscle tissues or real-time acquisition on electricsignals of cardiac muscle tissues. The chip device disclosed by the invention has the beneficial effects that the cardiac muscle cells are inoculated in the culture cavities and can grow and be anchored on two electrode masts to realize in-vitro three-dimensional culture of the cardiac muscle cells, so that the two electrodes are utilized for electrophysiological-signal acquisition and electrical-stimulation experiment and the like, and further the applications of drug screening and environmental-poison evaluation and the like can be carried out.

Description

technical field [0001] The invention belongs to the technical field of organ chips, and in particular relates to a chip device for three-dimensional culture of cells and in-situ real-time monitoring of myocardial tissue and its application. Background technique [0002] The heart is one of the most important organs in the human body. Its function is to push blood to the whole body, supply oxygen and nutrients and take away the end products of metabolism, so as to maintain the normal metabolism and function of various parts of the human body. Although modern medicine has made great progress in heart research, heart disease is still one of the main diseases causing death. [0003] The establishment of heart models in vitro provides a convenient solution for systematically studying the treatment of heart diseases. The bionic myocardial model can be applied to the research and development of relevant heart disease treatment drugs, and the effect of drugs on myocardial tissue ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42
CPCC12M23/34C12M25/14C12M35/02
Inventor 黄宁平张宁张峰
Owner SOUTHEAST UNIV
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