Chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and application thereof
A three-dimensional culture and real-time monitoring technology, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, methods for supporting/immobilizing microorganisms, etc. It can solve myocardial damage and cannot monitor real-time dynamic changes of myocardial tissue for a long time, etc. question
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Embodiment 1
[0031] Example 1 Study on three-dimensional growth of cardiomyocytes on a chip
[0032] like figure 1 As shown, the material of the substrate 1 and the cell culture chamber 2 is PDMS, the shape of the culture chamber 2 is dumbbell-shaped, the length is 8 mm, the width of the middle cell growth channel is 0.5 mm, and the height is 1 mm; the material of the bioelectrode 3 is platinum -PDMS composite, wherein the volume fraction of platinum content is 16%-22%, the obtained electrode has better conductivity (lower impedance), correspondingly, at 10Hz, the impedance of the platinum-PDMS composite is in the range of 16-2KΩ Inside; Cardiomyocytes derived from neonatal rats can be inoculated on the chip. In order to cultivate three-dimensional myocardial tissue, the number of cells inoculated in the culture room is 1.5×10 6 -2.5×10 6 range, such as figure 2 As shown in the pictures taken by the laser confocal microscope (CLSM), the three-dimensional culture of myocardial tissue on...
Embodiment 2
[0033] Example 2: Research on drug screening of cardiomyocytes on a chip
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