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Device on a chip for three-dimensional culture of cells and in situ real-time monitoring of myocardial tissue and its application

A three-dimensional culture and real-time monitoring technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, and methods of supporting/fixing microorganisms, etc. question

Active Publication Date: 2021-10-19
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, this optical monitoring method needs to take the whole device out of the constant temperature incubator, and use a microscope for "indirect" observation and recording. It cannot monitor the real-time dynamic changes of myocardial tissue for a long time, and long-term observation will have certain damage to the myocardium. damage

Method used

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  • Device on a chip for three-dimensional culture of cells and in situ real-time monitoring of myocardial tissue and its application
  • Device on a chip for three-dimensional culture of cells and in situ real-time monitoring of myocardial tissue and its application
  • Device on a chip for three-dimensional culture of cells and in situ real-time monitoring of myocardial tissue and its application

Examples

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Embodiment 1

[0031] Example 1 Study on three-dimensional growth of cardiomyocytes on a chip

[0032] Such as figure 1 As shown, the material of the substrate 1 and the cell culture chamber 2 is PDMS, the shape of the culture chamber 2 is dumbbell-shaped, the length is 8 mm, the width of the middle cell growth channel is 0.5 mm, and the height is 1 mm; the material of the bioelectrode 3 is platinum -PDMS composite, wherein the volume fraction of platinum content is 16%-22%, the obtained electrode has better conductivity (lower impedance), correspondingly, at 10Hz, the impedance of the platinum-PDMS composite is in the range of 16-2KΩ Inside; Cardiomyocytes derived from neonatal rats can be inoculated on the chip. In order to cultivate three-dimensional myocardial tissue, the number of cells inoculated in the culture room is 1.5×10 6 -2.5×10 6 range, such as figure 2 As shown in the pictures taken by the laser confocal microscope (CLSM), the three-dimensional culture of myocardial tissue...

Embodiment 2

[0033] Example 2: Research on drug screening of cardiomyocytes on a chip

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Abstract

The invention discloses a chip device for three-dimensional cell culture and in-situ real-time monitoring of myocardial tissue, which belongs to the technical field of organ chips, and includes a PDMS substrate, on which two cell culture chambers for culturing cardiomyocytes are arranged at intervals. The cell culture chambers are equipped with bio-electrodes correspondingly, and the two cell culture chambers are connected to each other; the bio-electrodes connect the chip with an external electrical stimulation device or an electrical signal acquisition device through the input or output of electrical signals respectively, so as to treat myocardial tissue. Electrical stimulation or real-time acquisition of electrical signals from myocardial tissue. The cardiomyocytes of the present invention are seeded in the culture chamber, can grow and be anchored on two electrode columns, and realize three-dimensional culture of cardiomyocytes in vitro, so that two electrodes can be used to collect electrophysiological signals and conduct electrical stimulation experiments on the myocardium. In turn, it can be used for drug screening, environmental toxicity evaluation and other applications.

Description

technical field [0001] The invention belongs to the technical field of organ chips, and in particular relates to a chip device for three-dimensional culture of cells and in-situ real-time monitoring of myocardial tissue and its application. Background technique [0002] The heart is one of the most important organs in the human body. Its function is to push blood to the whole body, supply oxygen and nutrients and take away the end products of metabolism, so as to maintain the normal metabolism and function of various parts of the human body. Although modern medicine has made great progress in heart research, heart disease is still one of the main diseases causing death. [0003] The establishment of heart models in vitro provides a convenient solution for systematically studying the treatment of heart diseases. The bionic myocardial model can be applied to the research and development of relevant heart disease treatment drugs, and the effect of drugs on myocardial tissue ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/42
CPCC12M23/34C12M25/14C12M35/02
Inventor 黄宁平张宁张峰
Owner SOUTHEAST UNIV
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