Human tissue-engineered cardiac muscle tissue

A tissue engineering and human technology, applied in the field of biomedical engineering, can solve problems such as poor mechanical properties and inflammatory reactions, and achieve the effects of reasonable porosity, good biocompatibility, and good cell compatibility

Inactive Publication Date: 2015-09-30
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Studies have shown that the above-mentioned materials for tissue engineering have the following defects: natural materials can only form a single layer of cardiomyocytes, have poor mechanical properties, and are difficult to meet some requirements for tissue construction; synthetic macromolecular materials can only be used as mechanical scaffolds for cells. Growth provides three-dimensional space, and its degradation products may trigger the body's inflammatory response

Method used

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  • Human tissue-engineered cardiac muscle tissue
  • Human tissue-engineered cardiac muscle tissue
  • Human tissue-engineered cardiac muscle tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Preparation of natural heart matrix

[0039] according to figure 1 As shown, using 1% SDS, 0.02% trypsin + EDTA, 1% Triton X-100, sterile PBS containing double antibody and sterilized water, the rat heart was perfused sequentially to obtain sterile decellularized natural Cardiac substrate (eg figure 2 shown);

[0040] 2) Make the obtained natural heart matrix into 1cm as needed 3 The size of the square substrate;

[0041] 3) In vitro culture of induced pluripotent stem cells and differentiated cardiomyocytes:

[0042] Special culture medium for induced pluripotent stem cells (purchased from STEMCELL Technologies or self-prepared), cultivated in a 5% carbon dioxide incubator at 37°C, and after the cells are congested, the promoters and inhibitors of the Wnt pathway are used in combination to induce cardiomyocytes Differentiation, beating cardiomyocytes can be seen after 7 days;

[0043] 4) Cardiomyocyte purification:

[0044] After 20 minutes of differentiatio...

Embodiment 2

[0052] The tissue-engineered human myocardial tissue prepared according to the method of Example 1 was cultured in vitro for a long time, and its contractile function and electrophysiological function were detected by means of multi-electrode microarray. The results showed that it had similar cell components and electrophysiological functions to normal human myocardial tissue function, add different small molecule chemical drugs or Chinese herbal medicine preparations to the myocardial tissue culture environment, and detect the changes in contractile function and electrophysiological function through a multi-electrode microarray system. The results show that the tested small molecule chemical drugs or Chinese herbal medicine can be predicted in advance. The possible functional effects and toxic reactions of the preparation on the heart tissue in the human body; Figure 6 It has been shown that the artificial human myocardial tissue has the same response to the drugs affecting t...

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Abstract

The invention belongs to the field of biomedical engineering, and relates to a human tissue-engineered cardiac muscle tissue and a preparation method thereof. The human tissue-engineered cardiac muscle tissue disclosed by the invention is prepared by inoculating all kinds of cell components of a normal heart differentiated from human induced multipotential stem cells by taking a natural decellularized heart substrate as the scaffold. The human tissue-engineered cardiac muscle tissue has cell components, extracellular matrixes and biological functions similar to that of a normal human cardiac muscle tissue; testing and screening of in-vitro drugs, initial in-vivo transplantation researches of clinical earlier-stage small animals and observation of treatment effects can be carried out; and helps can be finally offered for realizing individualized treatment of human cardiovascular diseases.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a tissue-engineered human myocardial tissue and a preparation method thereof. Background technique [0002] Tissue engineering is a new interdisciplinary subject emerging in the 21st century, and some people call it regenerative medicine. Its basic definition is to use biologically active substances to research and develop biological substitutes for repairing, maintaining, and promoting the functions and forms of various human tissues or organs after injury through in vitro culture or construction methods. In recent years, with the development of tissue engineering, its connotation has gradually expanded, and it has become an important means of regenerative medicine. Its core is to simulate the environment of in vivo tissue generation, culture cells in vitro, construct three-dimensional engineered tissues composed of cells and biomaterials with specific function...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/38A61L27/50A61L27/58C12N5/077
Inventor 孙宁汤其群陈思峰钱睿哲王青洁杨慧詹永坤李宾
Owner FUDAN UNIV
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